Exiguobacterium sibiricum (strain DSM 17290 / CIP 109462 / JCM 13490 / 255-15)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Bacillales Family XII. Incertae Sedis; Exiguobacterium; Exiguobacterium sibiricum

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3005 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1YF36|B1YF36_EXIS2 4a-hydroxytetrahydrobiopterin dehydratase OS=Exiguobacterium sibiricum (strain DSM 17290 / CIP 109462 / JCM 13490 / 255-15) OX=262543 GN=Exig_2814 PE=3 SV=1
MM1 pKa = 7.36IQMNVMIGFVSMSGNTEE18 pKa = 4.51DD19 pKa = 4.99IVHH22 pKa = 7.2LLQSEE27 pKa = 4.75LEE29 pKa = 4.08QKK31 pKa = 10.81GCIVHH36 pKa = 6.1IQEE39 pKa = 5.26LDD41 pKa = 3.01RR42 pKa = 11.84LYY44 pKa = 10.49PSDD47 pKa = 4.06FSQYY51 pKa = 11.15DD52 pKa = 3.39AVLLGSYY59 pKa = 7.99TWGDD63 pKa = 2.77GDD65 pKa = 4.75LPYY68 pKa = 10.48EE69 pKa = 4.49AEE71 pKa = 5.55DD72 pKa = 4.02FLEE75 pKa = 5.03DD76 pKa = 5.36LADD79 pKa = 3.97SDD81 pKa = 5.45LNGLAAASFGSGDD94 pKa = 3.42RR95 pKa = 11.84DD96 pKa = 3.68YY97 pKa = 11.3PKK99 pKa = 10.82YY100 pKa = 10.34CAAVDD105 pKa = 4.23LLEE108 pKa = 4.1QTLQSAGATITAPGLKK124 pKa = 9.26IEE126 pKa = 5.14FDD128 pKa = 3.69PDD130 pKa = 3.46TPEE133 pKa = 3.91KK134 pKa = 9.87QTACRR139 pKa = 11.84LFAEE143 pKa = 4.79EE144 pKa = 4.14FFEE147 pKa = 4.7RR148 pKa = 11.84AQRR151 pKa = 11.84LYY153 pKa = 11.25AA154 pKa = 4.5

Molecular weight: 17.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1YGB3|B1YGB3_EXIS2 Beta sliding clamp OS=Exiguobacterium sibiricum (strain DSM 17290 / CIP 109462 / JCM 13490 / 255-15) OX=262543 GN=Exig_0002 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 8.8PTFNPNNRR10 pKa = 11.84KK11 pKa = 9.18RR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.69VHH16 pKa = 5.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.8SGRR28 pKa = 11.84RR29 pKa = 11.84ILAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.38GRR39 pKa = 11.84KK40 pKa = 9.06ALTVV44 pKa = 3.3

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3005

0

3005

883705

30

1492

294.1

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.162 ± 0.046

0.578 ± 0.012

5.363 ± 0.033

7.092 ± 0.05

4.372 ± 0.039

6.937 ± 0.041

2.155 ± 0.025

6.735 ± 0.039

5.346 ± 0.047

10.449 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.021

3.262 ± 0.028

3.803 ± 0.025

4.281 ± 0.04

5.26 ± 0.039

5.628 ± 0.034

6.328 ± 0.036

7.348 ± 0.04

0.971 ± 0.017

3.29 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski