Mycobacterium ulcerans str. Harvey

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium ulcerans

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9033 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X8FFK7|X8FFK7_MYCUL Uncharacterized protein OS=Mycobacterium ulcerans str. Harvey OX=1299332 GN=I551_1975 PE=4 SV=1
MM1 pKa = 7.51GPFFNGVGGEE11 pKa = 4.04IGVVTSALIGGDD23 pKa = 3.76GAVLLSDD30 pKa = 3.85QIGGITGTLTSLPGLSGLQTLLPGLFPSSGDD61 pKa = 3.4TTTTPAPGGAWQQLFEE77 pKa = 4.37NTSSNLNALGTAWSEE92 pKa = 4.52DD93 pKa = 3.84PFPLLRR99 pKa = 11.84QVIANQQGYY108 pKa = 10.41AYY110 pKa = 9.52TLGQDD115 pKa = 3.83LSLAIQNLPTEE126 pKa = 4.51LAHH129 pKa = 6.85LPEE132 pKa = 5.67NIEE135 pKa = 3.94TGIQGFLAFNPGLYY149 pKa = 9.96AQQFVASTMNDD160 pKa = 2.56FHH162 pKa = 7.15TIGTSLSEE170 pKa = 4.37AGQDD174 pKa = 3.77LQIGLAGFPADD185 pKa = 3.6MQPAYY190 pKa = 10.11EE191 pKa = 4.9AFAAGNYY198 pKa = 9.13NLAVNDD204 pKa = 3.7ATKK207 pKa = 11.06ALLNVFITGFDD218 pKa = 3.47TSNLNDD224 pKa = 3.25IRR226 pKa = 11.84LLGPVADD233 pKa = 5.23LFPILALPGQDD244 pKa = 3.04VQGFSNLLPAGSIPAQMTHH263 pKa = 5.96NLANVLNALTDD274 pKa = 3.65TSISTTISGTLDD286 pKa = 3.38PPALVLDD293 pKa = 4.54ANFGLPLSILFGVAGAPVAGLDD315 pKa = 3.7GLATAGTTIGTGVASGNPFMVVGGLIDD342 pKa = 3.91APAVVLDD349 pKa = 4.0GFLNGEE355 pKa = 4.56TIVDD359 pKa = 3.49MSLPVTFDD367 pKa = 3.53VPVLGPLNIPVVIHH381 pKa = 6.69LPFQGLLVPPHH392 pKa = 7.53PITATVPLEE401 pKa = 3.78ILGVDD406 pKa = 3.36IPINLTLGGTQFGGWCPRR424 pKa = 11.84WW425 pKa = 3.25

Molecular weight: 43.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X8FN10|X8FN10_MYCUL Ammonium Transporter family protein OS=Mycobacterium ulcerans str. Harvey OX=1299332 GN=I551_0104 PE=4 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84LRR4 pKa = 11.84KK5 pKa = 9.48ARR7 pKa = 11.84AKK9 pKa = 9.42TVFRR13 pKa = 11.84LRR15 pKa = 11.84SRR17 pKa = 11.84PVRR20 pKa = 11.84HH21 pKa = 6.43PRR23 pKa = 11.84LQKK26 pKa = 9.98MSGRR30 pKa = 11.84LIKK33 pKa = 7.66TTPAPSPRR41 pKa = 11.84RR42 pKa = 11.84WTPWPPASS50 pKa = 3.85

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9033

0

9033

1677574

31

1470

185.7

19.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.97 ± 0.045

1.24 ± 0.012

5.57 ± 0.023

4.595 ± 0.029

2.879 ± 0.018

8.88 ± 0.043

2.346 ± 0.015

4.266 ± 0.018

2.06 ± 0.02

9.421 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.012

2.437 ± 0.024

6.076 ± 0.024

3.129 ± 0.017

8.018 ± 0.033

6.114 ± 0.021

6.151 ± 0.025

7.931 ± 0.027

1.664 ± 0.013

1.984 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski