Prochlorothrix hollandica PCC 9006 = CALU 1027

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochlorotrichaceae; Prochlorothrix; Prochlorothrix hollandica

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2PZ35|A0A0M2PZ35_PROHO CRISPR-associated protein Cmr6 OS=Prochlorothrix hollandica PCC 9006 = CALU 1027 OX=317619 GN=PROH_07105 PE=4 SV=1
MM1 pKa = 7.61SNLIITGVIDD11 pKa = 4.14GPLTGGTPKK20 pKa = 10.61AIEE23 pKa = 4.52LYY25 pKa = 10.46ALNDD29 pKa = 3.36IADD32 pKa = 3.94LSIYY36 pKa = 10.36GIEE39 pKa = 4.0AATNGSASTGVDD51 pKa = 3.82FTLTGSATAGDD62 pKa = 4.48YY63 pKa = 10.51IYY65 pKa = 10.9VASEE69 pKa = 3.99TTQFNAYY76 pKa = 10.12FGFNPTFVNAVANINGDD93 pKa = 3.33DD94 pKa = 4.01TIILFEE100 pKa = 4.68NNSIVDD106 pKa = 3.46VFGEE110 pKa = 4.03IGVDD114 pKa = 3.33GTGRR118 pKa = 11.84PWEE121 pKa = 4.63HH122 pKa = 6.85LDD124 pKa = 2.75GWAYY128 pKa = 10.91RR129 pKa = 11.84NNDD132 pKa = 3.33VDD134 pKa = 3.94PSSTFNASEE143 pKa = 3.98WTFSGVNALDD153 pKa = 4.73DD154 pKa = 4.45DD155 pKa = 4.85ASNVNATATPSWPIGTFTAGGTDD178 pKa = 3.35TTAPTIATLTPVDD191 pKa = 4.08DD192 pKa = 4.14SSNVVVGNNLQVQFSEE208 pKa = 4.29NVQKK212 pKa = 10.25GTGNIVIKK220 pKa = 10.55QSSDD224 pKa = 3.05NAVVEE229 pKa = 4.91TIDD232 pKa = 3.42VTSAQVTVSGSTVTINPTNDD252 pKa = 3.18LANSTGYY259 pKa = 10.5YY260 pKa = 10.17SSPKK264 pKa = 8.13WVVWCAPSGGTPHH277 pKa = 6.99QGFQPSRR284 pKa = 11.84SLQLII289 pKa = 3.97

Molecular weight: 30.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2Q252|A0A0M2Q252_PROHO Uncharacterized protein (Fragment) OS=Prochlorothrix hollandica PCC 9006 = CALU 1027 OX=317619 GN=PROH_05270 PE=4 SV=1
MM1 pKa = 7.56AVQIPRR7 pKa = 11.84VPPRR11 pKa = 11.84KK12 pKa = 9.75LPLLVRR18 pKa = 11.84LPLLARR24 pKa = 11.84LPLLARR30 pKa = 11.84LPLPQKK36 pKa = 10.51ALQPLKK42 pKa = 10.66VQAKK46 pKa = 8.51VKK48 pKa = 9.58FARR51 pKa = 11.84VLRR54 pKa = 11.84PLPTGTRR61 pKa = 11.84AMGTNGG67 pKa = 3.25

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3609

8

3617

1111657

20

2278

307.3

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.106 ± 0.047

1.004 ± 0.012

5.53 ± 0.028

5.436 ± 0.042

3.534 ± 0.028

7.681 ± 0.056

2.141 ± 0.023

5.091 ± 0.03

3.128 ± 0.04

11.81 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.015

3.07 ± 0.028

5.87 ± 0.049

5.922 ± 0.045

5.904 ± 0.033

5.997 ± 0.037

5.705 ± 0.034

6.879 ± 0.03

1.623 ± 0.022

2.735 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski