Tomato leaf curl Barka virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024DD88|A0A024DD88_9GEMI Replication-associated protein OS=Tomato leaf curl Barka virus OX=1498504 GN=C1 PE=3 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84INSFCVNAKK12 pKa = 10.44NIFLTYY18 pKa = 8.78PKK20 pKa = 10.44CPIPKK25 pKa = 9.32EE26 pKa = 3.87QMLEE30 pKa = 3.6ILQSIKK36 pKa = 10.77CPSDD40 pKa = 3.02KK41 pKa = 11.06LFIRR45 pKa = 11.84VSQEE49 pKa = 3.19KK50 pKa = 9.64HH51 pKa = 4.78QDD53 pKa = 3.22GSLHH57 pKa = 5.16IHH59 pKa = 6.91ALIQFKK65 pKa = 10.83GKK67 pKa = 10.55AKK69 pKa = 9.97FRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 6.16FDD77 pKa = 3.03VTHH80 pKa = 7.17PNNSSQFHH88 pKa = 7.01PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.55KK106 pKa = 10.86DD107 pKa = 2.8GDD109 pKa = 4.02YY110 pKa = 10.58IDD112 pKa = 4.14WGQFQIDD119 pKa = 3.44GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSAEE144 pKa = 4.04EE145 pKa = 3.85ALSIIRR151 pKa = 11.84EE152 pKa = 4.13KK153 pKa = 10.9LPKK156 pKa = 10.46DD157 pKa = 4.77FIFQYY162 pKa = 11.21HH163 pKa = 5.5NLKK166 pKa = 10.79CNLDD170 pKa = 4.1RR171 pKa = 11.84IFTPPVEE178 pKa = 4.6VYY180 pKa = 10.33VSPFLSSSFDD190 pKa = 3.45QVPEE194 pKa = 4.02EE195 pKa = 3.98LQEE198 pKa = 3.87WAAEE202 pKa = 4.17NVLGAAARR210 pKa = 11.84PLRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.12VYY251 pKa = 11.11NNDD254 pKa = 2.08AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.34KK312 pKa = 10.62EE313 pKa = 3.85FLDD316 pKa = 3.79EE317 pKa = 4.63EE318 pKa = 4.37KK319 pKa = 11.05NAALKK324 pKa = 10.72NWTLKK329 pKa = 10.5NATFITLEE337 pKa = 3.99RR338 pKa = 11.84PLYY341 pKa = 10.62SGSNQSAAQDD351 pKa = 3.81SQEE354 pKa = 3.99GDD356 pKa = 3.33QASTSS361 pKa = 3.49

Molecular weight: 40.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024DD88|A0A024DD88_9GEMI Replication-associated protein OS=Tomato leaf curl Barka virus OX=1498504 GN=C1 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.07SPYY27 pKa = 10.0SSRR30 pKa = 11.84AAVPICPSTNKK41 pKa = 9.3RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.3RR48 pKa = 11.84PMYY51 pKa = 9.36RR52 pKa = 11.84KK53 pKa = 9.08PRR55 pKa = 11.84IYY57 pKa = 10.75RR58 pKa = 11.84MYY60 pKa = 10.32RR61 pKa = 11.84SPDD64 pKa = 3.21VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 7.04EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.2DD83 pKa = 3.51IKK85 pKa = 9.71HH86 pKa = 5.35TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.31VTRR98 pKa = 11.84GSGITHH104 pKa = 6.35RR105 pKa = 11.84VGKK108 pKa = 9.5RR109 pKa = 11.84FCVKK113 pKa = 10.37SIYY116 pKa = 10.32FLGKK120 pKa = 9.15VWMDD124 pKa = 3.41EE125 pKa = 4.15NIKK128 pKa = 10.04KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.7RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.66GSSPMDD153 pKa = 3.83FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.85NDD173 pKa = 2.82LRR175 pKa = 11.84DD176 pKa = 3.36RR177 pKa = 11.84FQVMRR182 pKa = 11.84KK183 pKa = 7.65FHH185 pKa = 6.03ATVIGGPSGMKK196 pKa = 9.5EE197 pKa = 3.54QALVKK202 pKa = 10.31RR203 pKa = 11.84FFRR206 pKa = 11.84INSHH210 pKa = 3.89VTYY213 pKa = 10.54NHH215 pKa = 5.79QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 10.08ATMKK247 pKa = 9.61IRR249 pKa = 11.84IYY251 pKa = 10.55FYY253 pKa = 11.2DD254 pKa = 4.06SISNN258 pKa = 3.66

Molecular weight: 30.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1088

85

361

181.3

20.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.963 ± 0.968

2.022 ± 0.336

5.147 ± 0.422

4.596 ± 0.64

4.688 ± 0.569

4.688 ± 0.493

3.86 ± 0.685

6.71 ± 0.797

5.882 ± 0.556

6.158 ± 0.887

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.756

5.699 ± 0.558

6.25 ± 0.657

5.607 ± 0.691

6.71 ± 1.214

9.099 ± 1.546

5.055 ± 0.887

5.515 ± 0.83

1.195 ± 0.218

3.585 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski