Shigella phage Sf12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Rogunavirinae; Eastlansingvirus; Shigella virus Sf12

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291AXL7|A0A291AXL7_9CAUD Recombination protein OS=Shigella phage Sf12 OX=2024315 GN=Sf12_gp29 PE=4 SV=1
MM1 pKa = 7.59SIKK4 pKa = 9.36KK5 pKa = 7.48TLVWASVGGVDD16 pKa = 4.84HH17 pKa = 6.94GPFYY21 pKa = 11.09FDD23 pKa = 3.8VDD25 pKa = 3.66IGDD28 pKa = 4.06LEE30 pKa = 4.22YY31 pKa = 10.8GYY33 pKa = 11.05DD34 pKa = 3.89EE35 pKa = 5.95ISGSWYY41 pKa = 10.35GLSEE45 pKa = 4.31FSSILSNILNGYY57 pKa = 8.63RR58 pKa = 11.84LYY60 pKa = 11.29RR61 pKa = 11.84NFDD64 pKa = 3.17WYY66 pKa = 11.24NPEE69 pKa = 4.96DD70 pKa = 4.4GSDD73 pKa = 3.33GYY75 pKa = 11.66YY76 pKa = 10.5VLNEE80 pKa = 4.16GGVMEE85 pKa = 5.11PKK87 pKa = 10.19DD88 pKa = 4.24DD89 pKa = 4.11PFLRR93 pKa = 11.84EE94 pKa = 4.01GFNISWSDD102 pKa = 4.66LGDD105 pKa = 4.17DD106 pKa = 5.17DD107 pKa = 6.26YY108 pKa = 12.11DD109 pKa = 4.81FSRR112 pKa = 11.84TDD114 pKa = 3.1LRR116 pKa = 11.84VFYY119 pKa = 10.92SKK121 pKa = 11.01FIGDD125 pKa = 4.04YY126 pKa = 11.18DD127 pKa = 3.95DD128 pKa = 5.84DD129 pKa = 5.58KK130 pKa = 10.8YY131 pKa = 9.65TGSFGEE137 pKa = 4.81FFPEE141 pKa = 3.96DD142 pKa = 4.46WITVDD147 pKa = 4.61AYY149 pKa = 9.93TNSEE153 pKa = 3.32FDD155 pKa = 3.38MIYY158 pKa = 10.67EE159 pKa = 4.4LRR161 pKa = 11.84NGSNGEE167 pKa = 4.02VIKK170 pKa = 10.83PKK172 pKa = 9.45FTNDD176 pKa = 2.67

Molecular weight: 20.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291AXL1|A0A291AXL1_9CAUD Uncharacterized protein OS=Shigella phage Sf12 OX=2024315 GN=Sf12_gp18 PE=4 SV=1
MM1 pKa = 7.44IKK3 pKa = 9.02VTEE6 pKa = 3.92IDD8 pKa = 3.55KK9 pKa = 9.8RR10 pKa = 11.84TLNGNNGTIRR20 pKa = 11.84TEE22 pKa = 3.92DD23 pKa = 3.06KK24 pKa = 10.74KK25 pKa = 10.7PRR27 pKa = 11.84KK28 pKa = 9.27RR29 pKa = 11.84PSGYY33 pKa = 10.17YY34 pKa = 9.15VLKK37 pKa = 10.97DD38 pKa = 3.45EE39 pKa = 4.72VKK41 pKa = 9.74AGLRR45 pKa = 11.84ARR47 pKa = 11.84LEE49 pKa = 4.11IVLDD53 pKa = 4.33FYY55 pKa = 11.62GSKK58 pKa = 11.29ANMAKK63 pKa = 9.48QLKK66 pKa = 7.09VTRR69 pKa = 11.84QAVEE73 pKa = 3.54EE74 pKa = 4.23WFNRR78 pKa = 11.84GMMSARR84 pKa = 11.84GAQLAHH90 pKa = 5.93NRR92 pKa = 11.84YY93 pKa = 9.16KK94 pKa = 10.35RR95 pKa = 11.84TGEE98 pKa = 3.84GFRR101 pKa = 11.84ATFCRR106 pKa = 11.84PDD108 pKa = 3.38LQFDD112 pKa = 4.36GNGKK116 pKa = 8.92PLTLRR121 pKa = 11.84CKK123 pKa = 9.49KK124 pKa = 9.98RR125 pKa = 11.84HH126 pKa = 4.94MLRR129 pKa = 11.84VVTDD133 pKa = 3.75AEE135 pKa = 4.42LATKK139 pKa = 9.15PEE141 pKa = 4.04CRR143 pKa = 11.84SWRR146 pKa = 11.84KK147 pKa = 9.4IKK149 pKa = 10.36AANEE153 pKa = 3.74AARR156 pKa = 11.84KK157 pKa = 9.56AKK159 pKa = 9.88EE160 pKa = 3.61

Molecular weight: 18.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14230

41

1131

192.3

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.363 ± 0.561

1.117 ± 0.163

6.051 ± 0.257

6.627 ± 0.279

3.802 ± 0.202

8.025 ± 0.426

1.518 ± 0.211

6.247 ± 0.222

6.486 ± 0.411

7.133 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.24

5.249 ± 0.255

3.183 ± 0.276

3.865 ± 0.359

4.87 ± 0.254

6.683 ± 0.254

6.015 ± 0.319

7.154 ± 0.293

1.223 ± 0.118

3.774 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski