Scophthalmus maximus reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Aquareovirus; Aquareovirus E

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2WJL2|F2WJL2_9REOV NS22 OS=Scophthalmus maximus reovirus OX=994485 PE=4 SV=1
MM1 pKa = 7.55GNVQTSTNVYY11 pKa = 9.81NVNGDD16 pKa = 3.62NNAFTPTSEE25 pKa = 4.34MVASASPAIDD35 pKa = 4.04LKK37 pKa = 11.17PGVLNPNGKK46 pKa = 9.97LYY48 pKa = 10.51QLEE51 pKa = 4.52AGSAPDD57 pKa = 3.79PTNLILVVDD66 pKa = 4.61ASEE69 pKa = 5.01GDD71 pKa = 3.83FSYY74 pKa = 9.76LTNNTWEE81 pKa = 4.33TLSKK85 pKa = 10.73SALEE89 pKa = 4.26TNSWEE94 pKa = 4.06PMFSVTMTGCGPLKK108 pKa = 10.72LGDD111 pKa = 3.78YY112 pKa = 8.16TVTMSGYY119 pKa = 10.27VGASPSDD126 pKa = 3.49AFDD129 pKa = 3.72GGVIEE134 pKa = 5.44DD135 pKa = 4.03GSFISSRR142 pKa = 11.84RR143 pKa = 11.84LKK145 pKa = 10.61NFKK148 pKa = 10.69LMLSNRR154 pKa = 11.84CEE156 pKa = 5.41AIAKK160 pKa = 9.4WNMSLNQAMSLLTPDD175 pKa = 4.75LLAGSGSCKK184 pKa = 9.23WKK186 pKa = 10.8SVLQYY191 pKa = 7.74MQKK194 pKa = 10.08VLPSDD199 pKa = 4.07NEE201 pKa = 3.96VLQYY205 pKa = 10.62PDD207 pKa = 3.01EE208 pKa = 5.04FYY210 pKa = 10.59TVAVGKK216 pKa = 10.51YY217 pKa = 7.74PALKK221 pKa = 9.91PGSSPDD227 pKa = 3.79TPPPAAGPLGEE238 pKa = 4.64IACVMNAASASVGLMSGSSALLTSAMDD265 pKa = 3.52TLAAKK270 pKa = 10.48NLDD273 pKa = 4.12LVCAEE278 pKa = 4.49APLPVSTFTPSLAPRR293 pKa = 11.84DD294 pKa = 3.8YY295 pKa = 10.97RR296 pKa = 11.84PAFIKK301 pKa = 10.77DD302 pKa = 3.42ADD304 pKa = 4.02AHH306 pKa = 5.76WVTSITPTTYY316 pKa = 10.27FRR318 pKa = 11.84VTTTLSAKK326 pKa = 10.18NYY328 pKa = 9.78SVQLGPGATKK338 pKa = 10.62VLDD341 pKa = 3.75MNRR344 pKa = 11.84MVDD347 pKa = 3.78SDD349 pKa = 4.51LLLDD353 pKa = 3.87VSGMPIDD360 pKa = 3.69WMSNPDD366 pKa = 3.47YY367 pKa = 9.3ATSVAAIVLLEE378 pKa = 4.01SRR380 pKa = 11.84VPASEE385 pKa = 3.6ISAAEE390 pKa = 4.84DD391 pKa = 3.09ITGVSIVASSPLSIVNSTVNVRR413 pKa = 11.84GQHH416 pKa = 4.83FLEE419 pKa = 4.45MLHH422 pKa = 6.67LRR424 pKa = 11.84TTFEE428 pKa = 4.24RR429 pKa = 11.84EE430 pKa = 3.91TIAGKK435 pKa = 9.66PYY437 pKa = 10.11IYY439 pKa = 11.0GLGTLLLLSPTTASNSRR456 pKa = 11.84NPTLMDD462 pKa = 3.84GLLTITPILLRR473 pKa = 11.84DD474 pKa = 3.51TTYY477 pKa = 10.57KK478 pKa = 11.05GEE480 pKa = 4.12IVEE483 pKa = 4.99EE484 pKa = 4.14IVPSDD489 pKa = 2.97ILGNHH494 pKa = 6.02TSEE497 pKa = 4.18EE498 pKa = 4.15MAVALANDD506 pKa = 3.61AVVLMEE512 pKa = 4.3NSLKK516 pKa = 10.51EE517 pKa = 3.89VAEE520 pKa = 4.53VIGNAVPIASDD531 pKa = 3.87LDD533 pKa = 4.04DD534 pKa = 4.03SATASVVSRR543 pKa = 11.84LAITEE548 pKa = 4.21TASTRR553 pKa = 11.84SRR555 pKa = 11.84SNNPLAFPDD564 pKa = 6.09FGALWKK570 pKa = 9.74KK571 pKa = 10.56AKK573 pKa = 9.93RR574 pKa = 11.84AASLFVSNPKK584 pKa = 10.13SVLQVGVPVLASAGVIDD601 pKa = 4.97ALTSAVGTSVRR612 pKa = 11.84TGNIGKK618 pKa = 9.65GVQDD622 pKa = 3.91ALSILKK628 pKa = 10.07ARR630 pKa = 11.84NSVTKK635 pKa = 10.45LRR637 pKa = 11.84QGFFSKK643 pKa = 10.19IEE645 pKa = 4.01EE646 pKa = 4.31LWPVLEE652 pKa = 4.49GG653 pKa = 3.66

Molecular weight: 69.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2WJL3|F2WJL3_9REOV NS32 OS=Scophthalmus maximus reovirus OX=994485 PE=4 SV=1
MM1 pKa = 7.7GNTISSTFQYY11 pKa = 10.44TVLQIDD17 pKa = 4.33RR18 pKa = 11.84SCCIKK23 pKa = 10.13TSLTATSEE31 pKa = 4.18ATSWAIPPLAICCCCCLCCTGGLYY55 pKa = 10.33LVHH58 pKa = 6.9SGRR61 pKa = 11.84IPSISRR67 pKa = 11.84RR68 pKa = 11.84LDD70 pKa = 3.05VLRR73 pKa = 11.84DD74 pKa = 3.48TRR76 pKa = 11.84SASEE80 pKa = 3.81YY81 pKa = 10.08KK82 pKa = 10.44VRR84 pKa = 11.84SNRR87 pKa = 11.84NPKK90 pKa = 9.55SRR92 pKa = 11.84VRR94 pKa = 11.84RR95 pKa = 11.84VSISDD100 pKa = 3.64SSDD103 pKa = 3.1SSSLSDD109 pKa = 4.55LEE111 pKa = 4.31LSRR114 pKa = 11.84HH115 pKa = 5.61RR116 pKa = 11.84SHH118 pKa = 7.6PLAHH122 pKa = 6.54SFRR125 pKa = 11.84PEE127 pKa = 3.99SYY129 pKa = 9.2SQRR132 pKa = 11.84PHH134 pKa = 6.81SPSQAQSSIILPLVPVHH151 pKa = 6.56SRR153 pKa = 11.84TSLDD157 pKa = 3.37DD158 pKa = 3.67GVIRR162 pKa = 11.84SQPSRR167 pKa = 11.84YY168 pKa = 9.34QGPHH172 pKa = 4.07QQFEE176 pKa = 4.26DD177 pKa = 3.59WLQQAHH183 pKa = 7.19LLRR186 pKa = 11.84PGDD189 pKa = 3.6VSRR192 pKa = 11.84DD193 pKa = 3.7TNPFRR198 pKa = 6.11

Molecular weight: 22.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7756

198

1297

646.3

70.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.941 ± 0.492

1.199 ± 0.208

5.325 ± 0.176

3.262 ± 0.333

3.391 ± 0.252

5.609 ± 0.316

2.372 ± 0.219

5.08 ± 0.427

2.321 ± 0.39

10.031 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.214

4.139 ± 0.327

7.929 ± 0.383

3.829 ± 0.294

5.583 ± 0.281

8.394 ± 0.667

7.336 ± 0.337

7.452 ± 0.286

1.547 ± 0.199

2.888 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski