Microbacterium phage Lucky3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kojivirus; unclassified Kojivirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CFY3|A0A2P1CFY3_9CAUD Nuclease OS=Microbacterium phage Lucky3 OX=2099626 GN=32 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 4.26NTRR5 pKa = 11.84LKK7 pKa = 10.4LTGTTWSEE15 pKa = 3.62LLGAVTPTVIIVPGGEE31 pKa = 3.58RR32 pKa = 11.84FRR34 pKa = 11.84WDD36 pKa = 3.36EE37 pKa = 4.24SVGSYY42 pKa = 10.41CSEE45 pKa = 3.79SSGAGLYY52 pKa = 9.82IEE54 pKa = 5.53PSDD57 pKa = 4.14ADD59 pKa = 3.67GTITPDD65 pKa = 2.7SYY67 pKa = 11.98YY68 pKa = 9.32EE69 pKa = 3.91LPVIEE74 pKa = 4.92EE75 pKa = 4.34EE76 pKa = 4.53NNGG79 pKa = 3.56

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CGI9|A0A2P1CGI9_9CAUD Uncharacterized protein OS=Microbacterium phage Lucky3 OX=2099626 GN=58 PE=4 SV=1
MM1 pKa = 7.24NSSMKK6 pKa = 10.69AKK8 pKa = 10.08IAAITAAAEE17 pKa = 4.16AKK19 pKa = 10.67GIVQGGRR26 pKa = 11.84VRR28 pKa = 11.84LTGTEE33 pKa = 3.55RR34 pKa = 11.84TGTVKK39 pKa = 10.29RR40 pKa = 11.84VEE42 pKa = 4.17YY43 pKa = 10.93NDD45 pKa = 3.31IRR47 pKa = 11.84VIWDD51 pKa = 3.15EE52 pKa = 4.34GSRR55 pKa = 11.84QHH57 pKa = 6.62PSISATWTGLHH68 pKa = 6.36RR69 pKa = 11.84LEE71 pKa = 4.98PVTT74 pKa = 4.71

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12914

46

807

222.7

24.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.361 ± 0.426

0.604 ± 0.091

6.458 ± 0.315

6.241 ± 0.452

3.066 ± 0.241

8.077 ± 0.352

1.858 ± 0.171

4.491 ± 0.402

4.871 ± 0.347

8.541 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.127

3.136 ± 0.16

4.816 ± 0.221

4.29 ± 0.219

5.87 ± 0.399

5.862 ± 0.22

6.714 ± 0.261

7.627 ± 0.321

2.083 ± 0.222

2.718 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski