Lactococcus phage CHPC1182

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ceduovirus; Lactococcus virus CHPC1182

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650ERU3|A0A650ERU3_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC1182 OX=2675242 GN=CHPC1182_000297 PE=4 SV=1
MM1 pKa = 7.66AEE3 pKa = 3.91KK4 pKa = 10.59NIYY7 pKa = 9.19FVNDD11 pKa = 3.28EE12 pKa = 4.44VEE14 pKa = 4.24LKK16 pKa = 10.4QVLEE20 pKa = 5.19FISKK24 pKa = 8.89TDD26 pKa = 3.36YY27 pKa = 11.06GVNIDD32 pKa = 4.46KK33 pKa = 10.95SQEE36 pKa = 3.58DD37 pKa = 4.77VYY39 pKa = 11.7AVVTSYY45 pKa = 11.55SLPII49 pKa = 4.27

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650F767|A0A650F767_9CAUD Endolysin OS=Lactococcus phage CHPC1182 OX=2675242 GN=CHPC1182_000289 PE=3 SV=1
MM1 pKa = 7.32LTLLLTIIFIWLAFKK16 pKa = 9.98TVEE19 pKa = 4.17NVAEE23 pKa = 3.97EE24 pKa = 4.01LGRR27 pKa = 11.84YY28 pKa = 8.37IRR30 pKa = 11.84GFLKK34 pKa = 10.2WLWKK38 pKa = 9.36MYY40 pKa = 10.08KK41 pKa = 9.59KK42 pKa = 10.12HH43 pKa = 5.87INKK46 pKa = 9.45GVSLL50 pKa = 4.02

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

6208

48

628

182.6

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.944 ± 0.464

0.773 ± 0.187

5.928 ± 0.282

7.233 ± 0.5

4.478 ± 0.254

6.959 ± 0.491

1.466 ± 0.211

6.282 ± 0.453

8.65 ± 0.471

8.409 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.231

6.765 ± 0.326

2.577 ± 0.4

4.011 ± 0.338

3.479 ± 0.274

5.718 ± 0.462

6.169 ± 0.309

6.041 ± 0.444

1.418 ± 0.23

4.832 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski