Malpais Spring vesiculovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Vesiculovirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4VS08|M4VS08_9RHAB Matrix OS=Malpais Spring vesiculovirus OX=1972570 PE=3 SV=1
MM1 pKa = 7.27SKK3 pKa = 9.78IVEE6 pKa = 4.16LLKK9 pKa = 10.79NYY11 pKa = 9.17PNIMNTMEE19 pKa = 5.64DD20 pKa = 3.45IEE22 pKa = 4.42SLEE25 pKa = 4.46GEE27 pKa = 4.75VNPKK31 pKa = 9.67AASVVPGSSKK41 pKa = 10.89EE42 pKa = 4.1EE43 pKa = 3.85PTPSYY48 pKa = 10.71FLADD52 pKa = 3.63MLPEE56 pKa = 4.33EE57 pKa = 4.81EE58 pKa = 5.43GEE60 pKa = 4.16DD61 pKa = 3.4PKK63 pKa = 11.53GGVEE67 pKa = 3.84AGEE70 pKa = 4.6EE71 pKa = 4.14EE72 pKa = 5.07GSTAGDD78 pKa = 3.49YY79 pKa = 11.18DD80 pKa = 4.21DD81 pKa = 5.4YY82 pKa = 11.87AVQFEE87 pKa = 4.16DD88 pKa = 4.33RR89 pKa = 11.84EE90 pKa = 4.56WVAGTEE96 pKa = 3.99ISHH99 pKa = 7.45DD100 pKa = 3.77GTKK103 pKa = 10.08HH104 pKa = 4.27YY105 pKa = 11.14VITNPVKK112 pKa = 10.74SNRR115 pKa = 11.84EE116 pKa = 3.88LTKK119 pKa = 10.07KK120 pKa = 6.42WTAGIVGLLKK130 pKa = 10.49HH131 pKa = 6.84IEE133 pKa = 3.95EE134 pKa = 4.64GAGIKK139 pKa = 9.85INCEE143 pKa = 3.7EE144 pKa = 4.09VEE146 pKa = 4.16QGIKK150 pKa = 10.29CQMVTSCPPHH160 pKa = 6.93DD161 pKa = 5.22ANDD164 pKa = 3.93TSSSSGDD171 pKa = 3.61SEE173 pKa = 4.86TCSSPTSDD181 pKa = 3.27KK182 pKa = 11.21SKK184 pKa = 11.06NSTTISSVPDD194 pKa = 3.62FDD196 pKa = 4.36HH197 pKa = 7.32PSIIDD202 pKa = 4.4LIDD205 pKa = 4.32RR206 pKa = 11.84DD207 pKa = 3.71ITLPSLDD214 pKa = 3.68DD215 pKa = 3.81SKK217 pKa = 11.43PGFVLQLPRR226 pKa = 11.84LFGSRR231 pKa = 11.84EE232 pKa = 3.67AAINCCKK239 pKa = 10.36EE240 pKa = 4.01GEE242 pKa = 4.27VSIKK246 pKa = 10.22SAVVAGLRR254 pKa = 11.84RR255 pKa = 11.84KK256 pKa = 10.16GIYY259 pKa = 9.8NKK261 pKa = 9.68IRR263 pKa = 11.84IKK265 pKa = 10.76FDD267 pKa = 3.26LDD269 pKa = 3.65KK270 pKa = 11.25IQFF273 pKa = 3.63

Molecular weight: 29.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4W6E4|M4W6E4_9RHAB Nucleocapsid protein OS=Malpais Spring vesiculovirus OX=1972570 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.25SIKK5 pKa = 9.98KK6 pKa = 10.04VLGKK10 pKa = 10.42KK11 pKa = 9.48KK12 pKa = 10.57EE13 pKa = 4.02KK14 pKa = 10.77GEE16 pKa = 4.23KK17 pKa = 9.68KK18 pKa = 9.9SKK20 pKa = 10.65KK21 pKa = 9.65YY22 pKa = 10.39DD23 pKa = 3.44LPPNYY28 pKa = 9.74NDD30 pKa = 5.62LIGPSAPSAPMFGLDD45 pKa = 3.16PSDD48 pKa = 3.59YY49 pKa = 10.9FEE51 pKa = 5.24QINSNDD57 pKa = 3.43SVVIKK62 pKa = 10.45LKK64 pKa = 10.54YY65 pKa = 8.32SCEE68 pKa = 4.05VQVRR72 pKa = 11.84AIRR75 pKa = 11.84PFSGVLEE82 pKa = 4.37AADD85 pKa = 5.2AIARR89 pKa = 11.84WEE91 pKa = 3.61MDD93 pKa = 2.96YY94 pKa = 11.35RR95 pKa = 11.84GFLGKK100 pKa = 10.36KK101 pKa = 8.36PFYY104 pKa = 9.88RR105 pKa = 11.84LLMGIAIKK113 pKa = 10.35KK114 pKa = 9.52LRR116 pKa = 11.84AAPSSLTEE124 pKa = 4.09GNRR127 pKa = 11.84PEE129 pKa = 4.15YY130 pKa = 9.98NCLFEE135 pKa = 3.96GHH137 pKa = 6.0GAIRR141 pKa = 11.84HH142 pKa = 5.61NLGQLPPMSYY152 pKa = 10.52VSEE155 pKa = 4.29TFTRR159 pKa = 11.84DD160 pKa = 2.58WQTDD164 pKa = 3.38KK165 pKa = 11.63NKK167 pKa = 10.64GSVHH171 pKa = 4.71VKK173 pKa = 9.73FWLGMLDD180 pKa = 3.92TNDD183 pKa = 3.91EE184 pKa = 4.18MPEE187 pKa = 3.91ILSSKK192 pKa = 9.89SFQSEE197 pKa = 4.49SEE199 pKa = 4.16LKK201 pKa = 10.63QIMEE205 pKa = 4.04MMGIRR210 pKa = 11.84VKK212 pKa = 10.61KK213 pKa = 10.77SKK215 pKa = 10.3DD216 pKa = 3.54NKK218 pKa = 9.51WEE220 pKa = 3.98ILSTCC225 pKa = 4.03

Molecular weight: 25.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3539

225

2095

707.8

80.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.662 ± 0.77

1.978 ± 0.304

6.019 ± 0.563

6.019 ± 0.668

3.956 ± 0.333

6.047 ± 0.486

2.515 ± 0.195

7.573 ± 0.331

7.262 ± 0.493

9.268 ± 0.992

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.203

4.719 ± 0.615

5.199 ± 0.398

2.797 ± 0.14

4.832 ± 0.637

8.392 ± 0.475

5.736 ± 0.313

5.397 ± 0.419

1.893 ± 0.194

3.278 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski