Wenzhou tombus-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFE3|A0A1L3KFE3_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 18 OX=1923671 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84SIGTRR7 pKa = 11.84CVPSWLTHH15 pKa = 5.31KK16 pKa = 9.21TDD18 pKa = 4.01SGSSSPFEE26 pKa = 3.93TQGRR30 pKa = 11.84HH31 pKa = 5.57LLPPLLDD38 pKa = 3.85NLAPLPALTRR48 pKa = 11.84DD49 pKa = 3.44NQLAGIEE56 pKa = 4.15KK57 pKa = 10.0RR58 pKa = 11.84VLAVVPKK65 pKa = 8.49PHH67 pKa = 7.65PDD69 pKa = 3.26LVMEE73 pKa = 4.9DD74 pKa = 3.62QPKK77 pKa = 9.7PRR79 pKa = 11.84PFHH82 pKa = 6.46HH83 pKa = 7.04CPSVPTPEE91 pKa = 4.02RR92 pKa = 11.84SLIKK96 pKa = 10.77QMGDD100 pKa = 3.11LVASTGKK107 pKa = 9.86IPPVAAQPFLGIPPIYY123 pKa = 10.05LVPLSLMPDD132 pKa = 3.15DD133 pKa = 3.79WVEE136 pKa = 3.91EE137 pKa = 4.22VVDD140 pKa = 3.31IARR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84KK146 pKa = 9.52VQDD149 pKa = 3.88PMLPWPTAPTSADD162 pKa = 3.23LHH164 pKa = 6.76DD165 pKa = 4.99SSSCLLGPVTCRR177 pKa = 11.84TDD179 pKa = 3.36RR180 pKa = 11.84LNHH183 pKa = 5.93PFFRR187 pKa = 11.84CNLTSLVHH195 pKa = 6.0EE196 pKa = 5.04FWKK199 pKa = 10.63LRR201 pKa = 11.84EE202 pKa = 4.24NQWLTALEE210 pKa = 4.12PLPFEE215 pKa = 4.18EE216 pKa = 4.24WLEE219 pKa = 3.98RR220 pKa = 11.84FPNARR225 pKa = 11.84RR226 pKa = 11.84QQLRR230 pKa = 11.84DD231 pKa = 3.49ARR233 pKa = 11.84EE234 pKa = 3.3EE235 pKa = 4.1AYY237 pKa = 10.6RR238 pKa = 11.84HH239 pKa = 5.13GLEE242 pKa = 4.19SKK244 pKa = 10.25DD245 pKa = 3.62AIMAVFIKK253 pKa = 10.06TEE255 pKa = 4.07TSTSGTDD262 pKa = 3.28PRR264 pKa = 11.84NISPRR269 pKa = 11.84QLKK272 pKa = 9.59FLSVLGPFVAAIEE285 pKa = 4.27KK286 pKa = 9.59QAKK289 pKa = 8.74CCDD292 pKa = 3.66YY293 pKa = 10.87LVKK296 pKa = 10.73GKK298 pKa = 10.08NPEE301 pKa = 3.66EE302 pKa = 3.92RR303 pKa = 11.84GQYY306 pKa = 9.1ISQYY310 pKa = 7.69WRR312 pKa = 11.84HH313 pKa = 5.58SVIEE317 pKa = 4.12TDD319 pKa = 4.58FSRR322 pKa = 11.84FDD324 pKa = 3.47MTVSADD330 pKa = 3.49FVKK333 pKa = 9.86TVEE336 pKa = 3.65RR337 pKa = 11.84SMFRR341 pKa = 11.84RR342 pKa = 11.84AFPEE346 pKa = 3.94GLYY349 pKa = 10.0PALDD353 pKa = 3.68EE354 pKa = 5.3CLDD357 pKa = 4.33LLPVMTGASSLGVLYY372 pKa = 10.89SVAGTRR378 pKa = 11.84ASGDD382 pKa = 3.08AHH384 pKa = 5.82TSIANGVLNRR394 pKa = 11.84FVIWASLRR402 pKa = 11.84NMDD405 pKa = 3.78PSTWVSFHH413 pKa = 6.84EE414 pKa = 4.71GDD416 pKa = 5.21DD417 pKa = 4.75GIIHH421 pKa = 7.24CDD423 pKa = 3.22SAVTQQICDD432 pKa = 3.78YY433 pKa = 10.4LAFAAFLGFKK443 pKa = 9.99LKK445 pKa = 10.28VVRR448 pKa = 11.84PPSHH452 pKa = 5.84EE453 pKa = 3.8HH454 pKa = 6.59ANFCGRR460 pKa = 11.84HH461 pKa = 4.15TCEE464 pKa = 3.42EE465 pKa = 4.29CHH467 pKa = 7.33RR468 pKa = 11.84EE469 pKa = 4.0FCDD472 pKa = 4.78LPRR475 pKa = 11.84ALSKK479 pKa = 9.62FHH481 pKa = 6.36ITFKK485 pKa = 10.37QGEE488 pKa = 4.3PRR490 pKa = 11.84SLLLAKK496 pKa = 10.2ALSYY500 pKa = 10.4FATDD504 pKa = 2.42AHH506 pKa = 6.03TPIIGTMCDD515 pKa = 2.84ALIRR519 pKa = 11.84HH520 pKa = 6.9LSPQVSDD527 pKa = 2.79RR528 pKa = 11.84LMRR531 pKa = 11.84RR532 pKa = 11.84RR533 pKa = 11.84LHH535 pKa = 6.67AMSRR539 pKa = 11.84YY540 pKa = 10.05DD541 pKa = 3.29RR542 pKa = 11.84DD543 pKa = 3.19RR544 pKa = 11.84TIRR547 pKa = 11.84GMHH550 pKa = 5.66NTFMSPSACCRR561 pKa = 11.84AAISNVCGWDD571 pKa = 3.37PKK573 pKa = 11.14LQVAWEE579 pKa = 4.06AQLATWRR586 pKa = 11.84YY587 pKa = 8.88GITEE591 pKa = 4.34ISPLSVEE598 pKa = 4.24DD599 pKa = 4.26HH600 pKa = 6.94LVDD603 pKa = 3.64TDD605 pKa = 4.36SVTIYY610 pKa = 10.5QGG612 pKa = 2.97

Molecular weight: 69.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFE3|A0A1L3KFE3_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 18 OX=1923671 PE=4 SV=1
MM1 pKa = 7.23TGSNRR6 pKa = 11.84RR7 pKa = 11.84ANAGRR12 pKa = 11.84KK13 pKa = 6.07TQPKK17 pKa = 8.16PQRR20 pKa = 11.84KK21 pKa = 7.74PRR23 pKa = 11.84APRR26 pKa = 11.84RR27 pKa = 11.84PKK29 pKa = 9.62VQNAPRR35 pKa = 11.84IQQGGGPVPLLEE47 pKa = 5.01SNSNMRR53 pKa = 11.84QMHH56 pKa = 6.27NGMTRR61 pKa = 11.84VVGSDD66 pKa = 3.35YY67 pKa = 11.28LGVVSVAGNPADD79 pKa = 3.55AAAKK83 pKa = 8.57VRR85 pKa = 11.84KK86 pKa = 8.96VLSVSPSSFPGTRR99 pKa = 11.84LTQMSDD105 pKa = 2.08LWEE108 pKa = 4.37RR109 pKa = 11.84YY110 pKa = 6.56VFRR113 pKa = 11.84QFRR116 pKa = 11.84VRR118 pKa = 11.84YY119 pKa = 8.61VPSVPNTLACQVMVYY134 pKa = 10.46QDD136 pKa = 4.5TDD138 pKa = 3.66PQDD141 pKa = 3.88DD142 pKa = 3.65PTAIKK147 pKa = 10.74DD148 pKa = 3.26ADD150 pKa = 3.66ALLRR154 pKa = 11.84QATAQTGSQQWNFNSAKK171 pKa = 10.1VIHH174 pKa = 6.06LAKK177 pKa = 10.57RR178 pKa = 11.84SDD180 pKa = 3.3NQLYY184 pKa = 7.83YY185 pKa = 10.29TGPVKK190 pKa = 10.5EE191 pKa = 4.26NPRR194 pKa = 11.84FNQQGVVYY202 pKa = 8.22FIQVSQALDD211 pKa = 3.49MNGKK215 pKa = 8.74PLTADD220 pKa = 3.59MEE222 pKa = 4.82CGSLYY227 pKa = 10.58VDD229 pKa = 3.55WVIDD233 pKa = 3.84FQTPQVNPSAVEE245 pKa = 3.6ARR247 pKa = 11.84LPSAGFFTRR256 pKa = 11.84QILVNDD262 pKa = 4.04KK263 pKa = 9.76LTFAAQASLTVALTALSATPKK284 pKa = 10.05VWLEE288 pKa = 3.68GPTRR292 pKa = 11.84VDD294 pKa = 3.4LATLGGPGGNLVQIEE309 pKa = 4.35LTRR312 pKa = 11.84AQPGDD317 pKa = 3.65YY318 pKa = 8.83TVKK321 pKa = 10.46FVGCDD326 pKa = 3.01SVTLVSSAPIAA337 pKa = 3.82

Molecular weight: 36.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1389

337

612

463.0

51.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.423 ± 0.495

2.376 ± 0.442

5.688 ± 0.288

3.6 ± 0.882

3.384 ± 0.633

5.904 ± 0.635

2.592 ± 0.697

2.808 ± 0.95

3.816 ± 0.127

9.071 ± 0.513

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.356

3.168 ± 0.59

7.703 ± 0.091

4.824 ± 0.965

7.703 ± 0.308

7.703 ± 0.308

7.055 ± 0.789

7.991 ± 0.973

1.872 ± 0.21

2.304 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski