Labrys okinawensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Xanthobacteraceae; Labrys

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5818 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S9Q864|A0A2S9Q864_9RHIZ Aspartate-semialdehyde dehydrogenase OS=Labrys okinawensis OX=346911 GN=asd PE=3 SV=1
MM1 pKa = 8.02PSTSDD6 pKa = 3.29EE7 pKa = 4.27VLVPSTVDD15 pKa = 2.83VHH17 pKa = 6.43SAVDD21 pKa = 3.62AQIAAQDD28 pKa = 3.76LLAALKK34 pKa = 9.26QVCADD39 pKa = 4.56HH40 pKa = 5.97YY41 pKa = 10.59QSPAVVSFPINGATVLDD58 pKa = 4.02AMAVTGNFF66 pKa = 3.22

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S9Q406|A0A2S9Q406_9RHIZ Erythritol/L-threitol dehydrogenase OS=Labrys okinawensis OX=346911 GN=C5L14_28825 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATKK25 pKa = 10.3GGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.03GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5818

0

5818

1849414

28

2683

317.9

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.914 ± 0.039

0.809 ± 0.01

5.451 ± 0.025

5.454 ± 0.032

3.776 ± 0.022

8.747 ± 0.028

2.017 ± 0.016

5.274 ± 0.024

3.292 ± 0.027

10.499 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.012

2.477 ± 0.019

5.324 ± 0.025

3.13 ± 0.019

7.088 ± 0.041

5.408 ± 0.02

5.086 ± 0.023

7.307 ± 0.026

1.361 ± 0.013

2.258 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski