Shewanella frigidimarina (strain NCIMB 400)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella frigidimarina

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3990 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q086I6|Q086I6_SHEFN Carbamoyl-phosphate synthase large chain OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=carB PE=3 SV=1
MM1 pKa = 7.16HH2 pKa = 7.12TTNKK6 pKa = 10.26YY7 pKa = 9.49YY8 pKa = 10.77NQTILASSILMASLISGCNNDD29 pKa = 5.97DD30 pKa = 3.95NDD32 pKa = 3.86NDD34 pKa = 3.61NAATVPVINASNPINLAIDD53 pKa = 3.43VAINQQITATFSDD66 pKa = 3.64AMNEE70 pKa = 3.87QTINDD75 pKa = 3.47ASFTLTAPSQTAVLGTVSYY94 pKa = 11.51DD95 pKa = 3.78EE96 pKa = 4.6TSHH99 pKa = 5.91TAVFAPSSDD108 pKa = 3.53FTHH111 pKa = 5.71NTLYY115 pKa = 10.28QASITTEE122 pKa = 3.9VTNTAGIALASGFSWSFTTSALPDD146 pKa = 3.59VIAPQVVSNYY156 pKa = 9.88PVDD159 pKa = 4.02LAQDD163 pKa = 3.94FALNRR168 pKa = 11.84NMTAEE173 pKa = 4.14FSEE176 pKa = 4.4ALSPNTVNGSSFTLSDD192 pKa = 3.26GTNLVTGVVSVQGTVATFNPDD213 pKa = 3.28DD214 pKa = 3.93NLAANTLYY222 pKa = 10.31TATLTTAISDD232 pKa = 3.9LAIPANLLAASFSWSFTTSDD252 pKa = 3.11VAAIGPEE259 pKa = 4.04PVKK262 pKa = 10.81LRR264 pKa = 11.84TSGDD268 pKa = 3.36FAILTKK274 pKa = 10.49TGITNIPASAITGNIGSSPITAAAMDD300 pKa = 3.77NVFCNEE306 pKa = 3.53ISGTIYY312 pKa = 10.68GADD315 pKa = 3.23AAYY318 pKa = 8.74TGSGAITCFAGAAEE332 pKa = 4.81DD333 pKa = 3.72NTLVANAVLDD343 pKa = 3.43MGTAYY348 pKa = 10.99NDD350 pKa = 3.47AAGRR354 pKa = 11.84TLPDD358 pKa = 3.42YY359 pKa = 10.72TEE361 pKa = 5.19LGAGDD366 pKa = 3.67ISGMTLEE373 pKa = 4.76PGLYY377 pKa = 8.71KK378 pKa = 10.31WSSGVLISTDD388 pKa = 3.17VTLSGSANDD397 pKa = 3.16VWIFQIAGDD406 pKa = 4.29LTQAAASRR414 pKa = 11.84INLQSGAQAKK424 pKa = 10.31NIFWQVGGSAGVALDD439 pKa = 3.74TTAHH443 pKa = 6.06FEE445 pKa = 4.24GVILAEE451 pKa = 4.45KK452 pKa = 9.92GISINTDD459 pKa = 2.42ATVNGRR465 pKa = 11.84LMSQTAVTLDD475 pKa = 3.79HH476 pKa = 7.12NIVTQPTEE484 pKa = 3.67QFF486 pKa = 3.29

Molecular weight: 50.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q07VT7|Q07VT7_SHEFN ATP synthase I chain OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_4052 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.01VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3990

0

3990

1345936

36

5787

337.3

37.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.94 ± 0.045

1.034 ± 0.015

5.692 ± 0.042

5.459 ± 0.031

4.07 ± 0.027

6.635 ± 0.038

2.297 ± 0.021

6.67 ± 0.035

5.075 ± 0.036

10.484 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.023

4.485 ± 0.029

3.856 ± 0.023

4.989 ± 0.045

4.216 ± 0.036

6.739 ± 0.032

5.576 ± 0.036

6.895 ± 0.034

1.18 ± 0.016

3.031 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski