Pantoea phage LIMElight

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Limelightvirus; Pantoea virus Limelight

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1Y3T0|E1Y3T0_9CAUD Putative DNA helicase OS=Pantoea phage LIMElight OX=881915 PE=4 SV=1
MM1 pKa = 7.5GVLQDD6 pKa = 4.18GVSIQYY12 pKa = 10.29ISDD15 pKa = 3.55EE16 pKa = 4.23FPIMEE21 pKa = 4.25MQADD25 pKa = 4.57GYY27 pKa = 10.47EE28 pKa = 4.04RR29 pKa = 11.84HH30 pKa = 6.07NLQTGEE36 pKa = 4.04VDD38 pKa = 3.6TVQPTSPTLHH48 pKa = 6.45

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1Y3X9|E1Y3X9_9CAUD Uncharacterized protein OS=Pantoea phage LIMElight OX=881915 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 9.12ITRR5 pKa = 11.84RR6 pKa = 11.84HH7 pKa = 5.54IEE9 pKa = 3.76TVILVKK15 pKa = 10.67VALSVTFAAVYY26 pKa = 7.91FLPAPMAGAVGVASNLIWLWKK47 pKa = 9.13VV48 pKa = 2.9

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13655

48

1368

248.3

27.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.472 ± 0.526

0.879 ± 0.149

6.349 ± 0.321

5.595 ± 0.339

3.134 ± 0.178

7.711 ± 0.269

1.926 ± 0.154

4.702 ± 0.26

4.914 ± 0.288

8.1 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.171 ± 0.197

4.321 ± 0.301

3.691 ± 0.246

5.002 ± 0.419

5.763 ± 0.332

6.43 ± 0.287

6.466 ± 0.371

6.745 ± 0.327

1.296 ± 0.141

3.332 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski