Methylocucumis oryzae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylocucumis

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F3ILJ2|A0A0F3ILJ2_9GAMM Carboxynorspermidine/carboxyspermidine decarboxylase OS=Methylocucumis oryzae OX=1632867 GN=VZ94_05075 PE=3 SV=1
MM1 pKa = 7.3AVKK4 pKa = 10.64LGTLNDD10 pKa = 4.09DD11 pKa = 4.42DD12 pKa = 6.59LIGTAANDD20 pKa = 3.01VFLAHH25 pKa = 6.65SGKK28 pKa = 10.47DD29 pKa = 3.17ILIGNAGNDD38 pKa = 3.61LLYY41 pKa = 10.54FSSGSPTIFGGDD53 pKa = 3.29GNDD56 pKa = 3.58YY57 pKa = 10.79IVIEE61 pKa = 4.54GSTNAKK67 pKa = 9.54VDD69 pKa = 3.55AGSGNDD75 pKa = 3.04IVLASVSSSIGGVKK89 pKa = 10.24DD90 pKa = 4.13SPAHH94 pKa = 5.73TSLLDD99 pKa = 3.4TSGGDD104 pKa = 3.33GTDD107 pKa = 3.46YY108 pKa = 11.34LSLVLSGGYY117 pKa = 8.93QFSFTMGSGLNSTLYY132 pKa = 10.29TGTKK136 pKa = 9.39ISGFEE141 pKa = 3.59TGIIVGSSLGDD152 pKa = 3.29TFTATNWRR160 pKa = 11.84LDD162 pKa = 3.45NTIFTLPDD170 pKa = 3.7DD171 pKa = 4.03FCEE174 pKa = 4.39FLCANQKK181 pKa = 10.44CYY183 pKa = 11.17ADD185 pKa = 3.45

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F3IM83|A0A0F3IM83_9GAMM Uncharacterized protein OS=Methylocucumis oryzae OX=1632867 GN=VZ94_02250 PE=4 SV=1
MM1 pKa = 7.33ACIRR5 pKa = 11.84ATQEE9 pKa = 3.76QLPSRR14 pKa = 11.84DD15 pKa = 2.92NVAFTKK21 pKa = 10.49LYY23 pKa = 9.71RR24 pKa = 11.84LKK26 pKa = 10.93VCSQQMKK33 pKa = 9.57EE34 pKa = 4.11SLYY37 pKa = 10.99LPSCSFLRR45 pKa = 11.84SRR47 pKa = 11.84IAGHH51 pKa = 5.88VIIRR55 pKa = 11.84MHH57 pKa = 7.13PKK59 pKa = 8.59NTLTATRR66 pKa = 11.84ASTSNTT72 pKa = 3.24

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2947

0

2947

753327

29

2431

255.6

28.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.336 ± 0.06

1.125 ± 0.021

5.49 ± 0.045

5.467 ± 0.045

3.982 ± 0.032

6.624 ± 0.063

2.409 ± 0.03

6.334 ± 0.037

4.794 ± 0.047

11.106 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.024

4.51 ± 0.043

4.149 ± 0.032

4.798 ± 0.042

4.498 ± 0.039

6.39 ± 0.045

6.006 ± 0.062

6.442 ± 0.039

1.274 ± 0.019

3.113 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski