Pebjah virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Simarterivirinae; Iotaarterivirus; Pedartevirus; Deltaarterivirus pejah

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UIN6|A0A0G2UIN6_9NIDO Non-structural protein 3 OS=Pebjah virus OX=1658615 GN=ORF1a PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 4.98HH3 pKa = 6.33QVPLCLSVCLILCNCFHH20 pKa = 7.14LLWASDD26 pKa = 3.39NSSNSSSPSVCFRR39 pKa = 11.84FPRR42 pKa = 11.84LKK44 pKa = 10.5SVNFSIILRR53 pKa = 11.84AHH55 pKa = 5.56VCNLNDD61 pKa = 4.13SGILYY66 pKa = 10.53YY67 pKa = 9.3EE68 pKa = 4.38TQTHH72 pKa = 6.19SVGQSNSEE80 pKa = 4.01GGAKK84 pKa = 10.21CADD87 pKa = 3.67FSSDD91 pKa = 2.96GRR93 pKa = 11.84RR94 pKa = 11.84EE95 pKa = 3.92AARR98 pKa = 11.84HH99 pKa = 4.87GFGNVWVVPRR109 pKa = 11.84TVNTTFNFSDD119 pKa = 3.6QVGHH123 pKa = 6.41QGHH126 pKa = 5.15THH128 pKa = 5.94IAALLVYY135 pKa = 10.04LLSYY139 pKa = 9.13YY140 pKa = 10.03PRR142 pKa = 11.84VFNFTGNVTRR152 pKa = 11.84GYY154 pKa = 11.14YY155 pKa = 10.15LVEE158 pKa = 5.37DD159 pKa = 3.85DD160 pKa = 5.23TYY162 pKa = 11.79TSICVNGTVTPQEE175 pKa = 4.09RR176 pKa = 11.84MGLLALGVEE185 pKa = 4.98QEE187 pKa = 4.72IEE189 pKa = 3.73WATYY193 pKa = 9.67FPEE196 pKa = 4.28LLRR199 pKa = 11.84PFIFSVLLLAAAEE212 pKa = 4.13LL213 pKa = 4.03

Molecular weight: 23.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UPU7|A0A0G2UPU7_9NIDO E protein OS=Pebjah virus OX=1658615 GN=ORF2a PE=4 SV=1
MM1 pKa = 6.87VAKK4 pKa = 10.15ICSDD8 pKa = 3.37PGYY11 pKa = 6.67TTLAFTCAPVIIACLRR27 pKa = 11.84LFRR30 pKa = 11.84PALRR34 pKa = 11.84GFICAICIAILAYY47 pKa = 10.22AATAFSTHH55 pKa = 5.35SFATIVTLGFSLLYY69 pKa = 10.24LAYY72 pKa = 9.7KK73 pKa = 9.85LLQWAIIRR81 pKa = 11.84CRR83 pKa = 11.84MCRR86 pKa = 11.84LGRR89 pKa = 11.84GYY91 pKa = 8.39ITAPSSMIEE100 pKa = 4.18SSAGHH105 pKa = 6.11HH106 pKa = 6.88AIAASSSAVVKK117 pKa = 10.3RR118 pKa = 11.84RR119 pKa = 11.84PGSTTANGYY128 pKa = 8.8LVPDD132 pKa = 3.98VKK134 pKa = 10.74RR135 pKa = 11.84IILQGRR141 pKa = 11.84VAARR145 pKa = 11.84KK146 pKa = 9.61GLVNLRR152 pKa = 11.84RR153 pKa = 11.84YY154 pKa = 8.83GWQTKK159 pKa = 7.77TKK161 pKa = 10.33

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

8564

52

3552

570.9

62.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.921 ± 0.291

3.445 ± 0.118

4.39 ± 0.453

3.62 ± 0.282

4.005 ± 0.472

6.702 ± 0.455

2.604 ± 0.33

4.752 ± 0.289

3.596 ± 0.323

10.859 ± 0.445

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.448 ± 0.303

3.024 ± 0.306

6.656 ± 0.63

3.363 ± 0.174

5.418 ± 0.259

8.209 ± 0.518

5.92 ± 0.368

7.917 ± 0.38

1.378 ± 0.202

3.772 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski