Glis glis polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S1CJL7|A0A2S1CJL7_9POLY Large T antigen OS=Glis glis polyomavirus 1 OX=2170404 GN=650T PE=4 SV=1
MM1 pKa = 7.62LRR3 pKa = 11.84ILFQKK8 pKa = 9.91RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84GEE13 pKa = 3.86EE14 pKa = 3.5EE15 pKa = 4.2GPRR18 pKa = 11.84EE19 pKa = 4.02GGAAVAAAAAGNGGAPSDD37 pKa = 4.19TEE39 pKa = 3.93PTTPARR45 pKa = 11.84NIASIRR51 pKa = 11.84LQEE54 pKa = 4.18VAPGPEE60 pKa = 4.38DD61 pKa = 3.51KK62 pKa = 10.63PGPEE66 pKa = 4.21DD67 pKa = 3.62TSDD70 pKa = 3.6TEE72 pKa = 4.33DD73 pKa = 3.34TLGTEE78 pKa = 4.45DD79 pKa = 4.18TSDD82 pKa = 3.81TEE84 pKa = 4.41DD85 pKa = 3.59TPSPEE90 pKa = 3.45VWGEE94 pKa = 3.59YY95 pKa = 9.71LQYY98 pKa = 11.57

Molecular weight:
10.4 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S1CJL3|A0A2S1CJL3_9POLY Minor capsid protein VP2 OS=Glis glis polyomavirus 1 OX=2170404 GN=VP2 PE=3 SV=1
MM1 pKa = 7.61ALAPYY6 pKa = 10.24NPDD9 pKa = 2.62IDD11 pKa = 4.35YY12 pKa = 10.81DD13 pKa = 3.67ILFPGVSWFARR24 pKa = 11.84NIRR27 pKa = 11.84YY28 pKa = 8.54FDD30 pKa = 3.31PTFWTTRR37 pKa = 11.84LWQRR41 pKa = 11.84FVEE44 pKa = 4.53GLQRR48 pKa = 11.84EE49 pKa = 4.49AVRR52 pKa = 11.84QIAHH56 pKa = 5.66EE57 pKa = 4.22TNALGARR64 pKa = 11.84ARR66 pKa = 11.84EE67 pKa = 4.06QAALTLRR74 pKa = 11.84EE75 pKa = 3.68AVARR79 pKa = 11.84VVEE82 pKa = 4.11NTQWVFTNLGQGASNLYY99 pKa = 10.6ASVEE103 pKa = 4.06RR104 pKa = 11.84YY105 pKa = 9.14YY106 pKa = 11.56AEE108 pKa = 4.86LPGLNPAQRR117 pKa = 11.84RR118 pKa = 11.84ALMEE122 pKa = 4.15RR123 pKa = 11.84LPRR126 pKa = 11.84EE127 pKa = 4.58DD128 pKa = 3.53IVVPQQDD135 pKa = 4.29SLPQSGDD142 pKa = 3.16FVVRR146 pKa = 11.84YY147 pKa = 7.0EE148 pKa = 4.34APGGANQRR156 pKa = 11.84SCPNWLLPLILGIYY170 pKa = 7.28DD171 pKa = 3.98TTWGEE176 pKa = 4.21PVSKK180 pKa = 10.3HH181 pKa = 3.42ATKK184 pKa = 10.41RR185 pKa = 11.84KK186 pKa = 9.93SSAEE190 pKa = 3.72DD191 pKa = 3.09TGPKK195 pKa = 8.9TNCKK199 pKa = 9.65RR200 pKa = 11.84KK201 pKa = 9.8RR202 pKa = 11.84GSPGSKK208 pKa = 9.83NRR210 pKa = 11.84AA211 pKa = 3.29

Molecular weight:
23.88 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1801

98

650

300.2

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.385 ± 1.295

2.221 ± 0.554

5.33 ± 0.542

6.885 ± 0.584

3.776 ± 0.527

6.774 ± 0.908

1.943 ± 0.593

4.553 ± 0.369

4.942 ± 0.774

9.606 ± 0.644

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.53

4.997 ± 0.343

5.941 ± 0.794

4.442 ± 0.608

6.163 ± 0.54

5.497 ± 0.176

6.496 ± 0.355

5.386 ± 0.654

2.054 ± 0.257

3.054 ± 0.222

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski