Microvirga brassicacearum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N3PDC2|A0A5N3PDC2_9RHIZ Response regulator transcription factor OS=Microvirga brassicacearum OX=2580413 GN=FEZ63_10705 PE=4 SV=1
MM1 pKa = 7.84AIGSSIEE8 pKa = 4.48LIWHH12 pKa = 5.85FAGYY16 pKa = 9.98LHH18 pKa = 7.12LTQDD22 pKa = 3.93YY23 pKa = 8.39VASRR27 pKa = 11.84VRR29 pKa = 11.84LDD31 pKa = 4.5EE32 pKa = 5.28IIHH35 pKa = 6.28SNDD38 pKa = 2.65TAADD42 pKa = 3.77FRR44 pKa = 11.84VSADD48 pKa = 3.22EE49 pKa = 3.75YY50 pKa = 10.21LARR53 pKa = 11.84SEE55 pKa = 4.94APSDD59 pKa = 3.38MSNFAIQVKK68 pKa = 10.16YY69 pKa = 11.05SLGSEE74 pKa = 3.98NPDD77 pKa = 3.11RR78 pKa = 11.84GEE80 pKa = 3.96WADD83 pKa = 5.18LPDD86 pKa = 5.9DD87 pKa = 5.08DD88 pKa = 5.43SLDD91 pKa = 3.59VGAPNVDD98 pKa = 4.83LLPHH102 pKa = 6.77DD103 pKa = 4.16WSTGTSSLFAGQSYY117 pKa = 9.24VVSCTAEE124 pKa = 3.76SSGPGGSHH132 pKa = 6.37LQDD135 pKa = 3.96SQSHH139 pKa = 6.04PASTYY144 pKa = 8.42EE145 pKa = 4.31SKK147 pKa = 10.98SADD150 pKa = 3.55GGDD153 pKa = 3.44DD154 pKa = 3.8TIANLSQANIGADD167 pKa = 2.88RR168 pKa = 11.84DD169 pKa = 4.16YY170 pKa = 11.34FSDD173 pKa = 3.93GPNEE177 pKa = 4.42LGVLPHH183 pKa = 6.17VASDD187 pKa = 3.66QPAADD192 pKa = 3.76MLTLAEE198 pKa = 4.73EE199 pKa = 4.49YY200 pKa = 10.75NPTTMLPPGEE210 pKa = 4.64SVEE213 pKa = 4.4FWTSAVVDD221 pKa = 3.73RR222 pKa = 11.84HH223 pKa = 5.58EE224 pKa = 4.39SLAAGEE230 pKa = 4.73TEE232 pKa = 4.29AQTIAPGRR240 pKa = 11.84YY241 pKa = 8.78VNGKK245 pKa = 9.07LVALEE250 pKa = 4.36PSVEE254 pKa = 4.15TAQPANHH261 pKa = 6.97TLPEE265 pKa = 4.25PPVRR269 pKa = 11.84EE270 pKa = 4.2VGSTDD275 pKa = 3.25PAQIAEE281 pKa = 4.27TGGNKK286 pKa = 9.38LVNAATIADD295 pKa = 4.3LNEE298 pKa = 4.28APSTLIVGGDD308 pKa = 3.69YY309 pKa = 11.47YY310 pKa = 10.25EE311 pKa = 4.6TNAILQTNILQNQDD325 pKa = 3.38VVFHH329 pKa = 7.37DD330 pKa = 4.21GDD332 pKa = 4.33APPMLDD338 pKa = 3.27STGSTLDD345 pKa = 3.23NVANFVVEE353 pKa = 4.35EE354 pKa = 4.03LVKK357 pKa = 10.76QQGHH361 pKa = 5.84KK362 pKa = 10.59AGPLGNLTVNIDD374 pKa = 3.77YY375 pKa = 11.28VDD377 pKa = 3.62GHH379 pKa = 7.49VMDD382 pKa = 5.14VKK384 pKa = 11.17ALAQRR389 pKa = 11.84NYY391 pKa = 10.54FEE393 pKa = 6.16DD394 pKa = 4.51GDD396 pKa = 4.15VTVQTRR402 pKa = 11.84YY403 pKa = 10.55DD404 pKa = 3.85SYY406 pKa = 11.93SEE408 pKa = 3.74IHH410 pKa = 6.8SGDD413 pKa = 3.19NTQANVARR421 pKa = 11.84FADD424 pKa = 3.26WGKK427 pKa = 10.55DD428 pKa = 3.26YY429 pKa = 11.51DD430 pKa = 4.39VIIVMGDD437 pKa = 3.2YY438 pKa = 10.83HH439 pKa = 6.84SANLILQTNVVLDD452 pKa = 4.08DD453 pKa = 4.58DD454 pKa = 5.26YY455 pKa = 11.83IGISCGSGGSTPSIFSGQNALEE477 pKa = 4.21NEE479 pKa = 4.07ATIAKK484 pKa = 10.02YY485 pKa = 10.38GATTFGGINRR495 pKa = 11.84KK496 pKa = 9.24LDD498 pKa = 3.7SLIDD502 pKa = 4.08DD503 pKa = 4.74LNDD506 pKa = 4.48RR507 pKa = 11.84DD508 pKa = 4.94DD509 pKa = 4.76LDD511 pKa = 4.26RR512 pKa = 11.84NAWSDD517 pKa = 3.46FHH519 pKa = 6.54GASSGSLNVLFVTGNYY535 pKa = 9.72YY536 pKa = 10.67DD537 pKa = 4.98LNIISQINVLADD549 pKa = 3.36ADD551 pKa = 4.12FAIQGGSDD559 pKa = 2.73GWQWLSTGGNSALNQASIVNAGGVYY584 pKa = 9.65DD585 pKa = 3.54QHH587 pKa = 8.46LGGDD591 pKa = 4.34FYY593 pKa = 11.45EE594 pKa = 5.52DD595 pKa = 3.87SILVQANLMSDD606 pKa = 3.33GSEE609 pKa = 4.05AVATDD614 pKa = 3.55PTALVSEE621 pKa = 4.89LVAFMDD627 pKa = 3.68HH628 pKa = 6.3TPDD631 pKa = 3.59YY632 pKa = 10.6SDD634 pKa = 3.91SDD636 pKa = 4.15SVSWTKK642 pKa = 10.39DD643 pKa = 3.11TFGHH647 pKa = 6.92GDD649 pKa = 3.36TFGHH653 pKa = 5.89VLSS656 pKa = 4.75

Molecular weight: 70.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N3PD11|A0A5N3PD11_9RHIZ 2 3 4 5-tetrahydropyridine-2 6-dicarboxylate N-succinyltransferase OS=Microvirga brassicacearum OX=2580413 GN=dapD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.21VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.11GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4754

0

4754

1497474

16

2501

315.0

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.043 ± 0.048

0.785 ± 0.011

5.645 ± 0.029

5.547 ± 0.029

3.815 ± 0.021

8.595 ± 0.038

1.995 ± 0.017

5.443 ± 0.023

3.239 ± 0.033

10.237 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.016

2.655 ± 0.024

5.215 ± 0.028

3.027 ± 0.019

7.253 ± 0.036

5.618 ± 0.022

5.405 ± 0.027

7.593 ± 0.028

1.309 ± 0.015

2.199 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski