Chimpanzee stool associated circular ssDNA virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; unclassified Porprismacovirus

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2WKF8|D2WKF8_9VIRU Putative replicase protein OS=Chimpanzee stool associated circular ssDNA virus OX=702718 GN=rep PE=4 SV=1
MM1 pKa = 7.09STLLNRR7 pKa = 11.84LYY9 pKa = 10.72VGKK12 pKa = 10.3AGSTSAIAMNSVVAQNDD29 pKa = 3.03EE30 pKa = 4.21DD31 pKa = 3.95FGYY34 pKa = 10.73DD35 pKa = 2.97ASKK38 pKa = 11.2DD39 pKa = 3.35VDD41 pKa = 3.93QFAMYY46 pKa = 10.28YY47 pKa = 10.82GLLADD52 pKa = 3.49SDD54 pKa = 4.19GWRR57 pKa = 11.84KK58 pKa = 10.0AMPQAGLQMKK68 pKa = 9.13GLKK71 pKa = 9.88PLVFSMTTNFGQPSNVGLPNGAMVYY96 pKa = 9.23TPNGAGKK103 pKa = 10.49SSVEE107 pKa = 4.0NVADD111 pKa = 3.69STLFQYY117 pKa = 9.94MRR119 pKa = 11.84GPTMAMPALDD129 pKa = 4.08TFIQCSGDD137 pKa = 3.71TVPLGEE143 pKa = 5.19DD144 pKa = 3.39SVSPTPVPEE153 pKa = 4.07VSGSSVPALSGTVNMPYY170 pKa = 9.96SWCTPNIDD178 pKa = 3.65TPDD181 pKa = 3.51AFVAAIVLPPAKK193 pKa = 10.22LNRR196 pKa = 11.84LYY198 pKa = 11.4YY199 pKa = 10.02RR200 pKa = 11.84MKK202 pKa = 9.79VTWTVEE208 pKa = 4.11FSGLRR213 pKa = 11.84PDD215 pKa = 3.7TDD217 pKa = 3.35LTNWYY222 pKa = 9.43GLALAGVQSYY232 pKa = 10.8GSDD235 pKa = 3.95YY236 pKa = 10.0ITQTATIARR245 pKa = 11.84STSTANTSGMVDD257 pKa = 3.1TGDD260 pKa = 3.35VSLTKK265 pKa = 10.75VMEE268 pKa = 4.59GANN271 pKa = 3.48

Molecular weight: 28.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2WKF7|D2WKF7_9VIRU Putative capsid protein OS=Chimpanzee stool associated circular ssDNA virus OX=702718 PE=4 SV=1
MM1 pKa = 7.57AYY3 pKa = 10.02YY4 pKa = 9.9RR5 pKa = 11.84RR6 pKa = 11.84SYY8 pKa = 9.33RR9 pKa = 11.84RR10 pKa = 11.84SYY12 pKa = 9.93NRR14 pKa = 11.84RR15 pKa = 11.84PVRR18 pKa = 11.84RR19 pKa = 11.84SYY21 pKa = 10.92GKK23 pKa = 9.99KK24 pKa = 9.94VLPQVIRR31 pKa = 11.84MFVKK35 pKa = 10.42VSEE38 pKa = 4.52TYY40 pKa = 10.72DD41 pKa = 4.37LSTQTDD47 pKa = 2.71KK48 pKa = 11.16MGFVGIHH55 pKa = 4.75TPEE58 pKa = 4.3GKK60 pKa = 9.88LVYY63 pKa = 10.76NMWSGLFKK71 pKa = 10.78NFRR74 pKa = 11.84KK75 pKa = 9.39FRR77 pKa = 11.84YY78 pKa = 8.92ASCDD82 pKa = 3.08VTMACASMLPADD94 pKa = 4.26PLQIGVEE101 pKa = 4.09AGDD104 pKa = 4.21IAPQDD109 pKa = 3.63MFNPILYY116 pKa = 9.43KK117 pKa = 10.37AVSMTLCLRR126 pKa = 11.84CSIGSTSVRR135 pKa = 11.84LVLPPQSRR143 pKa = 3.38

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

1118

104

271

186.3

20.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.619 ± 0.884

2.236 ± 1.075

4.562 ± 0.891

3.488 ± 0.931

2.147 ± 0.456

7.245 ± 0.994

2.415 ± 1.217

5.456 ± 0.981

3.757 ± 0.497

7.871 ± 1.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.383 ± 0.319

3.399 ± 0.521

6.351 ± 0.726

3.757 ± 0.574

8.676 ± 2.382

8.587 ± 1.619

6.798 ± 0.948

6.53 ± 0.814

2.057 ± 0.8

3.667 ± 0.708
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski