Korarchaeum cryptofilum (strain OPF8)

Taxonomy: cellular organisms; Archaea; TACK group; Candidatus Korarchaeota; Candidatus Korarchaeum; Candidatus Korarchaeum cryptofilum

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1L530|B1L530_KORCO Lysine biosynthesis enzyme LysX OS=Korarchaeum cryptofilum (strain OPF8) OX=374847 GN=Kcr_0812 PE=3 SV=1
MM1 pKa = 7.04FASLGANMEE10 pKa = 4.3ARR12 pKa = 11.84CPICGSTLEE21 pKa = 4.57VPDD24 pKa = 4.29DD25 pKa = 5.01AIPGEE30 pKa = 4.87LIDD33 pKa = 5.22CNSCGALLEE42 pKa = 4.26VFNDD46 pKa = 3.18NGAISLRR53 pKa = 11.84EE54 pKa = 3.88AGGVLEE60 pKa = 5.05DD61 pKa = 3.44WGEE64 pKa = 3.73

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B1L6Q8|GGGPS_KORCO Geranylgeranylglyceryl phosphate synthase OS=Korarchaeum cryptofilum (strain OPF8) OX=374847 GN=Kcr_1391 PE=3 SV=2
MM1 pKa = 8.29LEE3 pKa = 3.9IFRR6 pKa = 11.84TGQLEE11 pKa = 3.98SSYY14 pKa = 11.61ARR16 pKa = 11.84LNPLVKK22 pKa = 10.49FILFITFIIIPLISTSFLLQLFSLVAQIPLILMSRR57 pKa = 11.84SGRR60 pKa = 11.84RR61 pKa = 11.84VVRR64 pKa = 11.84SLKK67 pKa = 10.65ASTFFILILIVLNYY81 pKa = 8.34ITTNSIVFSLSMVIRR96 pKa = 11.84LLTMIIASAIFMNGSNPSEE115 pKa = 4.2IGDD118 pKa = 3.79VLSKK122 pKa = 10.91LRR124 pKa = 11.84VPTSITFSFIVALRR138 pKa = 11.84FIPVLADD145 pKa = 3.24DD146 pKa = 4.97FMNIIASQASRR157 pKa = 11.84GHH159 pKa = 4.62EE160 pKa = 3.93VEE162 pKa = 3.49RR163 pKa = 11.84SGFIRR168 pKa = 11.84RR169 pKa = 11.84AKK171 pKa = 10.5SLLPLLIPLIVIAIRR186 pKa = 11.84RR187 pKa = 11.84AQQLAEE193 pKa = 4.12ALEE196 pKa = 4.41SRR198 pKa = 11.84CFGSGKK204 pKa = 7.44RR205 pKa = 11.84TSYY208 pKa = 8.77ITYY211 pKa = 10.42EE212 pKa = 3.68MGLIDD217 pKa = 6.11FLALVYY223 pKa = 9.35ATAIMITGSLLATLPPEE240 pKa = 4.17FLLLPFRR247 pKa = 4.93

Molecular weight: 27.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1602

0

1602

475001

47

2546

296.5

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.974 ± 0.066

0.845 ± 0.025

4.932 ± 0.037

8.157 ± 0.078

3.84 ± 0.037

7.787 ± 0.049

1.368 ± 0.021

8.199 ± 0.048

5.564 ± 0.05

10.877 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.026

2.822 ± 0.037

4.488 ± 0.039

1.532 ± 0.024

6.994 ± 0.064

7.328 ± 0.069

3.742 ± 0.045

7.294 ± 0.043

1.136 ± 0.021

3.465 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski