Bradyrhizobium sp. STM 3843

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0TFY1|H0TFY1_9BRAD Uncharacterized protein OS=Bradyrhizobium sp. STM 3843 OX=551947 GN=BRAS3843_1340043 PE=4 SV=1
MM1 pKa = 7.35SLSGALSSAISALNAQSSSLAMISDD26 pKa = 4.88NISNSDD32 pKa = 3.14TTGYY36 pKa = 8.23KK37 pKa = 7.21TTSAMFEE44 pKa = 4.13QLVTASSSSQSYY56 pKa = 9.97SSGGVSVSGRR66 pKa = 11.84SNITQQGLLASTTNATDD83 pKa = 3.24VAIQGSGFFVTANSTTAGGDD103 pKa = 3.15TFYY106 pKa = 10.89TRR108 pKa = 11.84NGAFTTDD115 pKa = 2.95NAGYY119 pKa = 10.62LEE121 pKa = 4.79DD122 pKa = 3.39NGYY125 pKa = 9.87YY126 pKa = 10.4LEE128 pKa = 4.18GWRR131 pKa = 11.84TDD133 pKa = 2.93ASGSITGTSLEE144 pKa = 4.54PINTKK149 pKa = 10.15VALTNGGATTKK160 pKa = 10.48TSISANLPADD170 pKa = 3.81ATSSSTFSSSTTVYY184 pKa = 11.02DD185 pKa = 3.87SLGDD189 pKa = 3.63AHH191 pKa = 6.54TLDD194 pKa = 3.94IKK196 pKa = 8.41WTKK199 pKa = 8.54TAANTWTAQMSSPDD213 pKa = 3.54GTISTTAPASDD224 pKa = 3.85TYY226 pKa = 11.76DD227 pKa = 3.11VTFNNDD233 pKa = 2.41GSLGTVTTAGQSTANPLSITWNNGAATSSITMDD266 pKa = 3.92FGTTGGGTNGLTQLSSGSTTPDD288 pKa = 2.56VSNFNASSDD297 pKa = 3.94GISFGTLSSISVGKK311 pKa = 10.4DD312 pKa = 2.94GIVDD316 pKa = 3.71ATYY319 pKa = 11.42SNGQTIPIYY328 pKa = 10.17KK329 pKa = 9.64IAVATFADD337 pKa = 4.14PNGLVAHH344 pKa = 6.62SGGMYY349 pKa = 9.95TSTATSGDD357 pKa = 3.24ATLQTSGEE365 pKa = 4.15NGAGTIYY372 pKa = 10.61GSEE375 pKa = 4.49LEE377 pKa = 4.51SSTTDD382 pKa = 3.1TTGQFSNMISAQQAYY397 pKa = 9.42SAASQVVTTVNKK409 pKa = 9.84MFDD412 pKa = 3.55TLISSMRR419 pKa = 3.49

Molecular weight: 42.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0THB4|H0THB4_9BRAD Putative alkanesulfonate ABC transporter permease protein OS=Bradyrhizobium sp. STM 3843 OX=551947 GN=BRAS3843_1490020 PE=3 SV=1
MM1 pKa = 6.94FRR3 pKa = 11.84VVAHH7 pKa = 6.83PLPLLAKK14 pKa = 10.26LRR16 pKa = 11.84RR17 pKa = 11.84LRR19 pKa = 11.84KK20 pKa = 9.76RR21 pKa = 11.84IAFAHH26 pKa = 6.13SATNGARR33 pKa = 11.84QMVRR37 pKa = 2.96

Molecular weight: 4.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8201

0

8201

2401352

12

4046

292.8

31.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.676 ± 0.04

0.922 ± 0.011

5.515 ± 0.023

5.174 ± 0.027

3.724 ± 0.019

8.275 ± 0.029

2.095 ± 0.016

5.287 ± 0.02

3.343 ± 0.023

10.085 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.388 ± 0.012

2.678 ± 0.017

5.345 ± 0.021

3.312 ± 0.014

7.101 ± 0.031

5.692 ± 0.023

5.353 ± 0.022

7.423 ± 0.022

1.357 ± 0.011

2.255 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski