Human immunodeficiency virus type 1 group M subtype C (isolate 92BR025) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:C

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O12164|ENV_HV192 Envelope glycoprotein gp160 OS=Human immunodeficiency virus type 1 group M subtype C (isolate 92BR025) OX=388812 GN=env PE=3 SV=1
MM1 pKa = 7.71LEE3 pKa = 4.15LIGRR7 pKa = 11.84IDD9 pKa = 3.37YY10 pKa = 10.76RR11 pKa = 11.84LGVGALIVALIIVIIVWTIAYY32 pKa = 9.21IEE34 pKa = 4.03YY35 pKa = 10.25RR36 pKa = 11.84KK37 pKa = 9.81LVRR40 pKa = 11.84QRR42 pKa = 11.84RR43 pKa = 11.84IDD45 pKa = 3.5WLVKK49 pKa = 10.02RR50 pKa = 11.84IKK52 pKa = 10.32EE53 pKa = 3.94RR54 pKa = 11.84AEE56 pKa = 3.98DD57 pKa = 3.58SGNEE61 pKa = 4.09SGGDD65 pKa = 3.64TEE67 pKa = 4.85EE68 pKa = 5.3LEE70 pKa = 4.46TMVDD74 pKa = 3.32MGHH77 pKa = 7.31LRR79 pKa = 11.84LLDD82 pKa = 4.17GNDD85 pKa = 3.15LL86 pKa = 3.91

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O12160|VPR_HV192 Protein Vpr OS=Human immunodeficiency virus type 1 group M subtype C (isolate 92BR025) OX=388812 GN=vpr PE=3 SV=1
MM1 pKa = 7.31EE2 pKa = 5.36NRR4 pKa = 11.84WQVLIVWQVDD14 pKa = 3.11RR15 pKa = 11.84MKK17 pKa = 10.64IRR19 pKa = 11.84TWNSLVKK26 pKa = 9.88HH27 pKa = 5.92HH28 pKa = 6.7MYY30 pKa = 9.98VSRR33 pKa = 11.84RR34 pKa = 11.84ASGWYY39 pKa = 8.66YY40 pKa = 8.46RR41 pKa = 11.84HH42 pKa = 6.65HH43 pKa = 6.78YY44 pKa = 9.38EE45 pKa = 4.47SRR47 pKa = 11.84HH48 pKa = 5.23PKK50 pKa = 9.62ISSEE54 pKa = 3.85VHH56 pKa = 5.47IPLGEE61 pKa = 3.97ARR63 pKa = 11.84LVIITYY69 pKa = 9.24WGLQTGEE76 pKa = 4.3RR77 pKa = 11.84DD78 pKa = 2.72WHH80 pKa = 6.47LGHH83 pKa = 6.56GVSIEE88 pKa = 3.6WRR90 pKa = 11.84LRR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 7.25STRR97 pKa = 11.84VDD99 pKa = 3.37PGLADD104 pKa = 3.54QLIHH108 pKa = 5.73MHH110 pKa = 6.16YY111 pKa = 9.71FDD113 pKa = 5.44CFADD117 pKa = 3.61SAIRR121 pKa = 11.84KK122 pKa = 9.2AILGHH127 pKa = 6.06RR128 pKa = 11.84VSSRR132 pKa = 11.84CDD134 pKa = 3.2YY135 pKa = 10.84QAGHH139 pKa = 6.29NKK141 pKa = 9.61VGSLQYY147 pKa = 10.82LALTALIKK155 pKa = 9.95PKK157 pKa = 10.13KK158 pKa = 9.28IKK160 pKa = 10.3PPLPSVKK167 pKa = 10.29KK168 pKa = 10.4LVEE171 pKa = 4.23DD172 pKa = 4.38RR173 pKa = 11.84WNKK176 pKa = 7.57PQKK179 pKa = 9.24TRR181 pKa = 11.84DD182 pKa = 3.11RR183 pKa = 11.84RR184 pKa = 11.84GNHH187 pKa = 5.15TMNGHH192 pKa = 6.29

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3643

72

1431

364.3

41.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.176 ± 0.51

2.059 ± 0.48

4.337 ± 0.301

6.753 ± 0.42

2.416 ± 0.183

7.302 ± 0.128

2.361 ± 0.38

6.945 ± 0.85

7.0 ± 1.077

8.153 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.243

4.859 ± 0.475

5.408 ± 0.845

5.71 ± 0.417

6.368 ± 1.132

5.078 ± 0.571

5.764 ± 0.443

5.655 ± 0.442

2.69 ± 0.266

2.635 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski