Lactococcus phage WRP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Audreyjarvisvirus; Lactococcus virus WRP3

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3MSR0|A0A0D3MSR0_9CAUD Uncharacterized protein OS=Lactococcus phage WRP3 OX=1560313 GN=WRP3_039 PE=4 SV=1
MM1 pKa = 7.76EE2 pKa = 4.34KK3 pKa = 9.75TKK5 pKa = 10.3EE6 pKa = 3.92YY7 pKa = 10.74NQITIDD13 pKa = 3.68VTEE16 pKa = 4.99LGLFSGLYY24 pKa = 9.4EE25 pKa = 4.75SIWLNSDD32 pKa = 2.84MDD34 pKa = 3.66IDD36 pKa = 4.16EE37 pKa = 4.37VMEE40 pKa = 4.34LADD43 pKa = 4.24MLGVDD48 pKa = 4.81CYY50 pKa = 11.05DD51 pKa = 3.33INVTIDD57 pKa = 3.21TQEE60 pKa = 3.78YY61 pKa = 9.86LEE63 pKa = 5.35AIGEE67 pKa = 4.42LYY69 pKa = 10.98CEE71 pKa = 4.35MFCNEE76 pKa = 4.62LDD78 pKa = 3.77SVGLFRR84 pKa = 11.84VDD86 pKa = 3.4SLYY89 pKa = 11.08SPKK92 pKa = 9.86WYY94 pKa = 10.76NFDD97 pKa = 3.42TDD99 pKa = 4.78HH100 pKa = 8.0IVITWDD106 pKa = 3.49SEE108 pKa = 4.4TLTVEE113 pKa = 4.04EE114 pKa = 4.89MEE116 pKa = 4.65SKK118 pKa = 10.64LKK120 pKa = 10.66EE121 pKa = 3.84LTADD125 pKa = 3.38NDD127 pKa = 3.78NRR129 pKa = 11.84DD130 pKa = 3.16DD131 pKa = 3.52WTIEE135 pKa = 3.82MEE137 pKa = 3.8LWDD140 pKa = 3.86YY141 pKa = 10.9RR142 pKa = 11.84GSEE145 pKa = 4.11IYY147 pKa = 11.1SNMVRR152 pKa = 11.84YY153 pKa = 7.93TYY155 pKa = 10.85NDD157 pKa = 3.22KK158 pKa = 10.71PLWFGMDD165 pKa = 3.6SNDD168 pKa = 3.04IAQVKK173 pKa = 8.98GG174 pKa = 3.38

Molecular weight: 20.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3MT50|A0A0D3MT50_9CAUD Uncharacterized protein OS=Lactococcus phage WRP3 OX=1560313 GN=WRP3_058 PE=4 SV=1
MM1 pKa = 7.08FKK3 pKa = 10.67KK4 pKa = 10.48RR5 pKa = 11.84ITRR8 pKa = 11.84LITVIVSYY16 pKa = 11.05LLLIGTYY23 pKa = 9.12WIYY26 pKa = 8.9PTIHH30 pKa = 6.25MGNNIFKK37 pKa = 10.61NITILLIGQPILFMFLIAFNTFIIAFLVFIACVIIYY73 pKa = 10.03KK74 pKa = 9.54IFKK77 pKa = 9.73WIMKK81 pKa = 9.97GNN83 pKa = 3.62

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

178

0

178

37621

44

3334

211.4

24.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.798 ± 0.325

0.649 ± 0.096

6.866 ± 0.156

8.099 ± 0.311

4.463 ± 0.215

5.691 ± 0.226

1.43 ± 0.126

7.708 ± 0.188

9.083 ± 0.202

8.198 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.647 ± 0.091

6.587 ± 0.159

2.289 ± 0.088

3.307 ± 0.269

3.541 ± 0.163

6.653 ± 0.246

6.209 ± 0.24

6.14 ± 0.152

1.074 ± 0.075

4.569 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski