Cohnella sp. OV330

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella; unclassified Cohnella

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0VHS7|A0A1I0VHS7_9BACL Dihydrofolate reductase OS=Cohnella sp. OV330 OX=1855288 GN=SAMN05216312_101318 PE=4 SV=1
MM1 pKa = 7.86PYY3 pKa = 9.21STGTVTNTRR12 pKa = 11.84DD13 pKa = 3.14FGTASTNIVLNVRR26 pKa = 11.84NLDD29 pKa = 2.99IALPVSVIVKK39 pKa = 9.51IFASVDD45 pKa = 2.92SDD47 pKa = 3.68NFYY50 pKa = 9.93TAYY53 pKa = 10.4VSSFDD58 pKa = 3.99VPPNAYY64 pKa = 9.81DD65 pKa = 3.23VRR67 pKa = 11.84TFFIAGNVAYY77 pKa = 9.13EE78 pKa = 4.11VQVDD82 pKa = 4.26LFSNSTNALISVYY95 pKa = 10.78GIDD98 pKa = 3.44EE99 pKa = 4.65FGNLVTDD106 pKa = 3.52QRR108 pKa = 11.84FAQNEE113 pKa = 4.01MSFIPTLSPNPP124 pKa = 3.76

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0XQ41|A0A1I0XQ41_9BACL Multiple sugar transport system substrate-binding protein OS=Cohnella sp. OV330 OX=1855288 GN=SAMN05216312_10370 PE=4 SV=1
MM1 pKa = 7.23QLEE4 pKa = 4.4SSEE7 pKa = 4.87SEE9 pKa = 3.89QKK11 pKa = 10.73NRR13 pKa = 11.84VLTLSDD19 pKa = 3.18TKK21 pKa = 10.72RR22 pKa = 11.84EE23 pKa = 3.76QRR25 pKa = 11.84RR26 pKa = 11.84QIRR29 pKa = 11.84LRR31 pKa = 11.84LAGRR35 pKa = 11.84ALARR39 pKa = 11.84IHH41 pKa = 6.2IVRR44 pKa = 11.84IGEE47 pKa = 3.97VRR49 pKa = 11.84PALPPAPVEE58 pKa = 4.4LVDD61 pKa = 5.98LSPAGCSFRR70 pKa = 11.84TGLRR74 pKa = 11.84FPVRR78 pKa = 11.84DD79 pKa = 3.47DD80 pKa = 3.11AVYY83 pKa = 10.42RR84 pKa = 11.84MEE86 pKa = 3.97WQLEE90 pKa = 3.96GMTMKK95 pKa = 10.52IKK97 pKa = 10.65GQLVWCRR104 pKa = 11.84EE105 pKa = 3.87DD106 pKa = 3.74EE107 pKa = 4.09YY108 pKa = 11.53GFRR111 pKa = 11.84YY112 pKa = 9.25GVKK115 pKa = 8.76FASGAVEE122 pKa = 4.34TILLVRR128 pKa = 11.84LLNALILKK136 pKa = 8.98ACPHH140 pKa = 6.09QGRR143 pKa = 11.84IHH145 pKa = 7.18RR146 pKa = 11.84IYY148 pKa = 10.6RR149 pKa = 11.84SQLDD153 pKa = 3.35HH154 pKa = 7.17LLRR157 pKa = 11.84RR158 pKa = 4.19

Molecular weight: 18.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6192

0

6192

2204178

29

4665

356.0

39.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.592 ± 0.047

0.668 ± 0.011

5.545 ± 0.024

6.291 ± 0.038

3.897 ± 0.023

8.247 ± 0.029

1.86 ± 0.016

5.547 ± 0.026

4.459 ± 0.027

10.029 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.017

3.244 ± 0.026

4.414 ± 0.019

3.298 ± 0.016

5.989 ± 0.044

6.173 ± 0.023

5.338 ± 0.042

7.031 ± 0.028

1.419 ± 0.013

3.445 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski