Sorghum chlorotic spot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Furovirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9JGJ4|Q9JGJ4_9VIRU Capsid protein OS=Sorghum chlorotic spot virus OX=107804 GN=CP PE=4 SV=1
WW1 pKa = 7.68GGVLSASRR9 pKa = 11.84EE10 pKa = 4.39SVVEE14 pKa = 3.77NAVLRR19 pKa = 11.84RR20 pKa = 11.84LTAKK24 pKa = 10.61DD25 pKa = 3.52LGLLPRR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.71TNGTVLQDD41 pKa = 3.89GPDD44 pKa = 3.07ILYY47 pKa = 10.82EE48 pKa = 4.04EE49 pKa = 5.52LEE51 pKa = 4.46DD52 pKa = 5.67ALLASNTSVYY62 pKa = 10.31NPHH65 pKa = 6.78AAIEE69 pKa = 4.31EE70 pKa = 4.39YY71 pKa = 10.57EE72 pKa = 4.05IDD74 pKa = 3.73TGDD77 pKa = 3.87GEE79 pKa = 4.41QLRR82 pKa = 11.84HH83 pKa = 5.35EE84 pKa = 4.6VSHH87 pKa = 6.61TEE89 pKa = 3.86DD90 pKa = 2.8SDD92 pKa = 3.76YY93 pKa = 11.18RR94 pKa = 11.84ILYY97 pKa = 9.91LFLILCAILMTLAMMLSRR115 pKa = 11.84AFVNFLYY122 pKa = 10.43NRR124 pKa = 11.84RR125 pKa = 11.84DD126 pKa = 3.05HH127 pKa = 6.9RR128 pKa = 11.84RR129 pKa = 11.84FGGPCEE135 pKa = 3.82RR136 pKa = 11.84CARR139 pKa = 11.84RR140 pKa = 11.84LWRR143 pKa = 11.84FRR145 pKa = 11.84LWLVGLAMRR154 pKa = 11.84RR155 pKa = 11.84YY156 pKa = 10.59GDD158 pKa = 3.45LQVTRR163 pKa = 11.84KK164 pKa = 9.02RR165 pKa = 11.84HH166 pKa = 4.18TRR168 pKa = 11.84SAMRR172 pKa = 11.84QYY174 pKa = 11.12LNSYY178 pKa = 9.58EE179 pKa = 4.28DD180 pKa = 4.33QTDD183 pKa = 2.98LWQSYY188 pKa = 10.17EE189 pKa = 3.93GVTPEE194 pKa = 4.02EE195 pKa = 4.04ALIVADD201 pKa = 3.13QHH203 pKa = 6.71LFVDD207 pKa = 5.18AVDD210 pKa = 3.85QLASGGGDD218 pKa = 3.48LQCVRR223 pKa = 11.84TAQTISEE230 pKa = 4.43RR231 pKa = 11.84YY232 pKa = 8.3GVKK235 pKa = 10.49NPALQDD241 pKa = 3.54KK242 pKa = 10.32LDD244 pKa = 3.68EE245 pKa = 4.33FARR248 pKa = 11.84RR249 pKa = 11.84LAIQVRR255 pKa = 11.84FEE257 pKa = 4.53DD258 pKa = 3.73TDD260 pKa = 3.56ATPGFLDD267 pKa = 3.96LRR269 pKa = 11.84NASMQEE275 pKa = 3.89VLDD278 pKa = 4.64CDD280 pKa = 5.15LSEE283 pKa = 4.12QTINPQYY290 pKa = 11.04NQICALQHH298 pKa = 4.7MFAMVGKK305 pKa = 8.92PAFTDD310 pKa = 3.87FVIAYY315 pKa = 8.45KK316 pKa = 9.35GTYY319 pKa = 7.8YY320 pKa = 10.31PCVIEE325 pKa = 4.26AQRR328 pKa = 11.84KK329 pKa = 6.59HH330 pKa = 8.2AEE332 pKa = 4.08MLGTDD337 pKa = 4.82PDD339 pKa = 4.6DD340 pKa = 3.16KK341 pKa = 10.62WGPLEE346 pKa = 3.9WMYY349 pKa = 10.95CVIMVGSLASFGFATVKK366 pKa = 10.9GIVKK370 pKa = 10.03LSQMVKK376 pKa = 8.56NWLRR380 pKa = 11.84LRR382 pKa = 11.84ALRR385 pKa = 11.84ALPSVTHH392 pKa = 6.5GGDD395 pKa = 3.36GGDD398 pKa = 3.26GGLPFFEE405 pKa = 5.04MEE407 pKa = 4.73DD408 pKa = 3.31FTARR412 pKa = 11.84LRR414 pKa = 11.84VLNEE418 pKa = 3.58FVANMGEE425 pKa = 4.28QVVDD429 pKa = 3.46TSVEE433 pKa = 3.91DD434 pKa = 3.56LRR436 pKa = 11.84RR437 pKa = 11.84DD438 pKa = 3.52LVSVLTAHH446 pKa = 6.8SGSEE450 pKa = 4.03PPAVDD455 pKa = 3.15RR456 pKa = 11.84PYY458 pKa = 11.33LEE460 pKa = 4.21GVGIGRR466 pKa = 11.84EE467 pKa = 4.09VDD469 pKa = 3.5EE470 pKa = 5.57LPASTEE476 pKa = 3.78IEE478 pKa = 4.33GEE480 pKa = 4.05QGEE483 pKa = 4.82SLFDD487 pKa = 3.93PGLRR491 pKa = 11.84IKK493 pKa = 10.5RR494 pKa = 11.84WEE496 pKa = 4.05TEE498 pKa = 3.55AAVPKK503 pKa = 10.59GVGGFSSFIRR513 pKa = 11.84TSGSVSGRR521 pKa = 11.84ARR523 pKa = 11.84MSAQNSVSRR532 pKa = 11.84VKK534 pKa = 10.68LRR536 pKa = 11.84NASMMKK542 pKa = 10.05SKK544 pKa = 11.02RR545 pKa = 11.84LMAAA549 pKa = 3.44

Molecular weight: 61.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9JGJ3|Q9JGJ3_9VIRU 62-kDa readthrough protein (Fragment) OS=Sorghum chlorotic spot virus OX=107804 GN=RT PE=4 SV=1
MM1 pKa = 6.92TVSTIHH7 pKa = 5.49SCEE10 pKa = 3.67RR11 pKa = 11.84CLEE14 pKa = 4.04GRR16 pKa = 11.84TSLRR20 pKa = 11.84CEE22 pKa = 3.49NKK24 pKa = 9.57YY25 pKa = 10.58RR26 pKa = 11.84LSVYY30 pKa = 8.61QSRR33 pKa = 11.84QVEE36 pKa = 4.23KK37 pKa = 10.47SAYY40 pKa = 8.57ACKK43 pKa = 10.01ISQFGVPCGMPAQFEE58 pKa = 4.62LDD60 pKa = 3.6GEE62 pKa = 4.55TLKK65 pKa = 10.94VVCDD69 pKa = 4.97GYY71 pKa = 11.48CGLKK75 pKa = 9.3HH76 pKa = 6.77KK77 pKa = 11.04NMAEE81 pKa = 3.99SGSWRR86 pKa = 11.84GTLLVILQKK95 pKa = 10.55EE96 pKa = 4.27LEE98 pKa = 4.09ALQLKK103 pKa = 9.76EE104 pKa = 4.2EE105 pKa = 4.05QLKK108 pKa = 8.82TRR110 pKa = 11.84IAEE113 pKa = 4.0VTQQHH118 pKa = 6.71DD119 pKa = 3.73LVMAEE124 pKa = 4.07TAAVLRR130 pKa = 11.84PDD132 pKa = 4.28SPPKK136 pKa = 11.11AMVTTNSRR144 pKa = 11.84VKK146 pKa = 9.96YY147 pKa = 9.86VRR149 pKa = 11.84RR150 pKa = 11.84KK151 pKa = 8.03PAPRR155 pKa = 11.84MM156 pKa = 3.56

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

2991

156

1277

498.5

56.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.954 ± 0.806

1.906 ± 0.314

6.653 ± 0.361

7.322 ± 0.213

4.313 ± 0.547

5.583 ± 0.563

1.705 ± 0.249

4.447 ± 0.5

6.687 ± 0.922

8.76 ± 0.6

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.976 ± 0.404

4.346 ± 0.402

3.243 ± 0.298

3.644 ± 0.32

6.553 ± 0.504

6.653 ± 0.324

5.517 ± 0.483

8.124 ± 0.569

1.17 ± 0.12

3.444 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski