cyanobacterium endosymbiont of Rhopalodia gibberula

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; unclassified Cyanobacteria

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5SX87|A0A2Z5SX87_9CYAN Uncharacterized protein OS=cyanobacterium endosymbiont of Rhopalodia gibberula OX=1763363 GN=RGRSB_0656 PE=4 SV=1
MM1 pKa = 7.67DD2 pKa = 5.76LEE4 pKa = 4.55YY5 pKa = 10.37PDD7 pKa = 4.73DD8 pKa = 4.33LRR10 pKa = 11.84YY11 pKa = 9.97LDD13 pKa = 3.31SHH15 pKa = 6.4EE16 pKa = 4.27YY17 pKa = 9.89IRR19 pKa = 11.84LDD21 pKa = 4.11GEE23 pKa = 3.87IATIGISAYY32 pKa = 10.32AIEE35 pKa = 4.17QLGDD39 pKa = 3.4IVFLEE44 pKa = 4.42LPEE47 pKa = 4.64VGDD50 pKa = 4.11SIEE53 pKa = 3.97VGEE56 pKa = 4.25VFGAIEE62 pKa = 4.2SVKK65 pKa = 10.41AAEE68 pKa = 4.33DD69 pKa = 4.19LYY71 pKa = 10.31PPVSGTVVDD80 pKa = 4.78RR81 pKa = 11.84NEE83 pKa = 4.09QMVEE87 pKa = 4.09TPEE90 pKa = 4.29QIADD94 pKa = 3.74DD95 pKa = 4.61PYY97 pKa = 11.52GDD99 pKa = 3.45GWLLKK104 pKa = 10.72VRR106 pKa = 11.84VEE108 pKa = 4.24NLDD111 pKa = 3.6DD112 pKa = 4.86HH113 pKa = 7.4LDD115 pKa = 3.73NTLSASEE122 pKa = 4.13YY123 pKa = 7.81QAQVEE128 pKa = 4.41GEE130 pKa = 4.17EE131 pKa = 4.17

Molecular weight: 14.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5SZW9|A0A2Z5SZW9_9CYAN Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD OS=cyanobacterium endosymbiont of Rhopalodia gibberula OX=1763363 GN=gatD PE=3 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 7.61QKK14 pKa = 9.91RR15 pKa = 11.84KK16 pKa = 9.02SGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TQNGRR29 pKa = 11.84NVIQARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.03KK38 pKa = 9.85GRR40 pKa = 11.84YY41 pKa = 8.24RR42 pKa = 11.84LSVV45 pKa = 3.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1671

0

1671

545903

29

1981

326.7

36.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.725 ± 0.052

1.088 ± 0.021

4.684 ± 0.039

6.264 ± 0.062

3.972 ± 0.044

6.768 ± 0.055

1.924 ± 0.031

7.716 ± 0.054

5.588 ± 0.056

11.218 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.916 ± 0.024

4.381 ± 0.047

4.536 ± 0.043

4.948 ± 0.051

4.992 ± 0.049

6.431 ± 0.047

5.727 ± 0.037

6.6 ± 0.049

1.378 ± 0.027

3.147 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski