Human immunodeficiency virus type 1 group M subtype F1 (isolate 93BR020) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:F

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O89290|POL_HV193 Gag-Pol polyprotein OS=Human immunodeficiency virus type 1 group M subtype F1 (isolate 93BR020) OX=388814 GN=gag-pol PE=3 SV=3
MM1 pKa = 7.57SNLLAIGIAALIVALIITIVVWTIAYY27 pKa = 9.18IEE29 pKa = 4.2YY30 pKa = 10.34KK31 pKa = 10.52KK32 pKa = 10.65LVRR35 pKa = 11.84QRR37 pKa = 11.84KK38 pKa = 8.64INRR41 pKa = 11.84LYY43 pKa = 10.89KK44 pKa = 10.25RR45 pKa = 11.84ISEE48 pKa = 4.21RR49 pKa = 11.84AEE51 pKa = 3.87DD52 pKa = 3.73SGNEE56 pKa = 4.08SEE58 pKa = 5.67GDD60 pKa = 3.77AEE62 pKa = 4.07EE63 pKa = 4.33LAALGEE69 pKa = 4.37VGPFIPGDD77 pKa = 3.58INNLL81 pKa = 3.47

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O70891|VPU_HV193 Protein Vpu OS=Human immunodeficiency virus type 1 group M subtype F1 (isolate 93BR020) OX=388814 GN=vpu PE=3 SV=1
MM1 pKa = 7.87AGRR4 pKa = 11.84SGDD7 pKa = 3.66SDD9 pKa = 4.02QEE11 pKa = 3.87LLKK14 pKa = 10.62AVRR17 pKa = 11.84YY18 pKa = 9.81IKK20 pKa = 10.12ILYY23 pKa = 8.96QSNPYY28 pKa = 9.06PKK30 pKa = 10.22PEE32 pKa = 3.93GTRR35 pKa = 11.84QARR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84WRR46 pKa = 11.84ARR48 pKa = 11.84QRR50 pKa = 11.84QIRR53 pKa = 11.84EE54 pKa = 3.4ISDD57 pKa = 4.39RR58 pKa = 11.84ILSSCLGRR66 pKa = 11.84PAEE69 pKa = 4.22PVPLQLPPLEE79 pKa = 4.39RR80 pKa = 11.84LHH82 pKa = 6.73INCSEE87 pKa = 4.26DD88 pKa = 3.3CGQGAEE94 pKa = 4.2EE95 pKa = 5.01GVGSSQISGEE105 pKa = 4.11SHH107 pKa = 5.84TVLGSGTKK115 pKa = 9.05EE116 pKa = 3.63

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3632

72

1430

363.2

41.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.36 ± 0.431

1.982 ± 0.43

4.047 ± 0.298

7.021 ± 0.471

2.506 ± 0.173

7.599 ± 0.26

2.203 ± 0.446

6.553 ± 0.618

7.241 ± 0.908

8.398 ± 0.543

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.257

4.488 ± 0.852

5.589 ± 0.791

5.92 ± 0.479

6.03 ± 0.708

5.011 ± 0.393

5.892 ± 0.392

5.644 ± 0.396

2.698 ± 0.249

2.753 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski