Citrus clementina (Clementine) (Citrus deliciosa x Citrus sinensis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4T4Y3|V4T4Y3_CITCL Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10001904mg PE=4 SV=1
MM1 pKa = 7.78AIIRR5 pKa = 11.84DD6 pKa = 3.63RR7 pKa = 11.84TEE9 pKa = 3.65DD10 pKa = 3.34FKK12 pKa = 11.79DD13 pKa = 3.93VVRR16 pKa = 11.84HH17 pKa = 4.98AAVYY21 pKa = 10.75DD22 pKa = 3.85EE23 pKa = 5.93FIGFCFWDD31 pKa = 5.4GYY33 pKa = 9.69DD34 pKa = 4.72QEE36 pKa = 5.25NAPDD40 pKa = 3.54WASNHH45 pKa = 5.55AQLNIGISDD54 pKa = 4.54LNNSTNSEE62 pKa = 3.63DD63 pKa = 3.07WFAGFEE69 pKa = 4.63LEE71 pKa = 4.36CCWVYY76 pKa = 10.91GWNN79 pKa = 3.6

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4S392|V4S392_CITCL Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10006168mg PE=3 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84PNNKK6 pKa = 9.59LIKK9 pKa = 9.81FLAVFLIVIVLMSPIAAAQRR29 pKa = 11.84GGGGGGGGGRR39 pKa = 11.84GGGGSGGGGGSRR51 pKa = 11.84GGGGGGSRR59 pKa = 11.84PIGGGTPTRR68 pKa = 11.84GGGRR72 pKa = 11.84PVNGGTPAGGGRR84 pKa = 11.84IPIGGGTVTRR94 pKa = 11.84GGGNGNAGGSSGVGRR109 pKa = 11.84GNMAKK114 pKa = 10.46LSLTTISLLSYY125 pKa = 10.01VAFILLHH132 pKa = 5.9GALL135 pKa = 4.31

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24934

6339

31273

12651335

10

5178

404.5

45.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.743 ± 0.013

1.916 ± 0.007

5.252 ± 0.01

6.382 ± 0.016

4.342 ± 0.009

6.434 ± 0.013

2.359 ± 0.006

5.467 ± 0.008

6.129 ± 0.014

9.958 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.005

4.63 ± 0.011

4.763 ± 0.013

3.695 ± 0.009

5.218 ± 0.011

8.949 ± 0.017

4.773 ± 0.008

6.505 ± 0.01

1.278 ± 0.005

2.825 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski