Murine coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7B990|I7B990_9BETC Nucleoprotein OS=Murine coronavirus OX=694005 GN=N PE=3 SV=1
MM1 pKa = 7.47LFVFILLLPSCLGYY15 pKa = 10.36IGDD18 pKa = 4.3FRR20 pKa = 11.84CIQTVNYY27 pKa = 9.86NGNNASAPSISTEE40 pKa = 3.73AVDD43 pKa = 3.61VSKK46 pKa = 11.15GRR48 pKa = 11.84GTYY51 pKa = 9.22YY52 pKa = 11.01VLDD55 pKa = 3.67RR56 pKa = 11.84VYY58 pKa = 11.47LNATLLLTGYY68 pKa = 10.58YY69 pKa = 9.74PVDD72 pKa = 3.26GSNYY76 pKa = 9.77RR77 pKa = 11.84NLALTGTNTLSLTWFKK93 pKa = 11.13PPFLSEE99 pKa = 4.04FNDD102 pKa = 5.06GIFAKK107 pKa = 10.4VQNLKK112 pKa = 9.78TNTPTGATSYY122 pKa = 10.66FPTIVIGSLFGNTSYY137 pKa = 10.68TVVLEE142 pKa = 4.17PYY144 pKa = 10.14NNIIMASVCTYY155 pKa = 7.55TICQLPYY162 pKa = 10.03TPCKK166 pKa = 10.23PNTNGNRR173 pKa = 11.84VIGFWHH179 pKa = 7.05TDD181 pKa = 3.24VKK183 pKa = 11.12PPICLLKK190 pKa = 10.88RR191 pKa = 11.84NFTFNVNAPWLYY203 pKa = 10.39FHH205 pKa = 7.79FYY207 pKa = 8.91QQGGTFYY214 pKa = 10.77AYY216 pKa = 10.83YY217 pKa = 10.42ADD219 pKa = 4.72KK220 pKa = 10.83PSATTFLFSVYY231 pKa = 10.07IGDD234 pKa = 3.89ILTQYY239 pKa = 10.48FVLPFICTPTAGSTLAPLYY258 pKa = 9.98WVTPLLKK265 pKa = 10.15RR266 pKa = 11.84QYY268 pKa = 10.93LFNFNEE274 pKa = 4.21KK275 pKa = 10.49GVITSAVDD283 pKa = 3.33CASSYY288 pKa = 10.66ISEE291 pKa = 4.54IKK293 pKa = 10.69CKK295 pKa = 8.53TQSLLPSTGVYY306 pKa = 10.24DD307 pKa = 3.93LSGYY311 pKa = 6.78TVQPVGVVYY320 pKa = 10.46RR321 pKa = 11.84RR322 pKa = 11.84VPNLPDD328 pKa = 3.5CKK330 pKa = 9.93IEE332 pKa = 3.81EE333 pKa = 4.32WLTAKK338 pKa = 10.27SVPSPLNWEE347 pKa = 3.58RR348 pKa = 11.84RR349 pKa = 11.84TFQNCNFNLSSLLRR363 pKa = 11.84YY364 pKa = 8.69VQAEE368 pKa = 4.13SLSCNNIDD376 pKa = 3.31ASKK379 pKa = 10.66VYY381 pKa = 9.95GMCFGSVSVDD391 pKa = 2.67KK392 pKa = 10.62FAIPRR397 pKa = 11.84SRR399 pKa = 11.84QIDD402 pKa = 3.88LQIGNSGFLQTANYY416 pKa = 9.74KK417 pKa = 9.91IDD419 pKa = 3.64TAATSCQLYY428 pKa = 10.65YY429 pKa = 10.87SLPKK433 pKa = 10.92NNVTINNYY441 pKa = 9.54NPSSWNRR448 pKa = 11.84RR449 pKa = 11.84YY450 pKa = 10.28GFKK453 pKa = 11.13VNDD456 pKa = 3.59RR457 pKa = 11.84CQIFANILLNGINSGTTCSTDD478 pKa = 3.14LQLPNTEE485 pKa = 4.21VATGVCVRR493 pKa = 11.84YY494 pKa = 9.74DD495 pKa = 3.44LYY497 pKa = 11.29GITGQGVFKK506 pKa = 10.25EE507 pKa = 4.64VKK509 pKa = 9.62ADD511 pKa = 4.25YY512 pKa = 10.05YY513 pKa = 11.52NSWQALLYY521 pKa = 10.55DD522 pKa = 4.03VNGNLNGFRR531 pKa = 11.84DD532 pKa = 3.65LTTNKK537 pKa = 9.29TYY539 pKa = 10.09TIRR542 pKa = 11.84SCYY545 pKa = 9.26SGRR548 pKa = 11.84VSAAYY553 pKa = 9.67HH554 pKa = 6.17KK555 pKa = 8.08EE556 pKa = 3.96APEE559 pKa = 3.78PALLYY564 pKa = 10.9RR565 pKa = 11.84NINCSYY571 pKa = 10.95VFTNNISRR579 pKa = 11.84EE580 pKa = 4.24EE581 pKa = 3.99NPLNYY586 pKa = 9.38FDD588 pKa = 5.71SYY590 pKa = 10.97LGCVVNADD598 pKa = 3.38NRR600 pKa = 11.84TDD602 pKa = 3.5EE603 pKa = 5.07ALPNCDD609 pKa = 3.43LRR611 pKa = 11.84MGAGLCVDD619 pKa = 3.67YY620 pKa = 11.11SKK622 pKa = 11.14SRR624 pKa = 11.84RR625 pKa = 11.84ARR627 pKa = 11.84RR628 pKa = 11.84SVSTGYY634 pKa = 11.01RR635 pKa = 11.84LTTFEE640 pKa = 5.37PYY642 pKa = 9.99MPMLVNDD649 pKa = 4.28SVQSVGGLYY658 pKa = 10.45EE659 pKa = 3.97MQIPTNFTIGHH670 pKa = 6.13HH671 pKa = 5.77EE672 pKa = 4.09EE673 pKa = 4.92FIQIRR678 pKa = 11.84APKK681 pKa = 8.75VTIDD685 pKa = 3.67CAAFVCGDD693 pKa = 3.4NAACRR698 pKa = 11.84QQLVEE703 pKa = 4.55YY704 pKa = 10.87GSFCDD709 pKa = 4.0NVNAILNEE717 pKa = 3.97VNNLLDD723 pKa = 4.08NMQLQVASALMQGVTISSRR742 pKa = 11.84LPDD745 pKa = 5.17GISGPIDD752 pKa = 4.86DD753 pKa = 5.34INFSPLLGCIGSTCAEE769 pKa = 4.69DD770 pKa = 4.47GNGPSAIRR778 pKa = 11.84GRR780 pKa = 11.84SAIEE784 pKa = 4.11DD785 pKa = 3.76LLFDD789 pKa = 4.06KK790 pKa = 11.25VKK792 pKa = 10.82LSDD795 pKa = 3.34VGFVEE800 pKa = 6.19AYY802 pKa = 9.87NNCTGGQEE810 pKa = 4.32VRR812 pKa = 11.84DD813 pKa = 3.97LLCVQSFNGIKK824 pKa = 10.05VLPPVLSEE832 pKa = 4.18SQISGYY838 pKa = 7.58TAGATAAAMFPPWTAAAGVPFSLNVQYY865 pKa = 10.59RR866 pKa = 11.84INGLGVTMNVLSEE879 pKa = 4.11NQKK882 pKa = 10.06MIASAFNNALGAIQEE897 pKa = 4.62GFDD900 pKa = 3.73ATNSALGKK908 pKa = 9.37IQSVVNANAEE918 pKa = 4.13ALNNLLNQLSNRR930 pKa = 11.84FGAISASLQEE940 pKa = 4.0ILTRR944 pKa = 11.84LDD946 pKa = 3.15AVEE949 pKa = 5.13AKK951 pKa = 10.57AQIDD955 pKa = 3.56RR956 pKa = 11.84LINGRR961 pKa = 11.84LTALNAYY968 pKa = 9.21ISKK971 pKa = 9.99QLSDD975 pKa = 3.23STLIKK980 pKa = 10.46FSAAQAIEE988 pKa = 4.11KK989 pKa = 10.61VNEE992 pKa = 4.28CVKK995 pKa = 10.93SQTTRR1000 pKa = 11.84INFCGNGNHH1009 pKa = 6.86ILSLVQNAPYY1019 pKa = 9.9GLCFIHH1025 pKa = 6.84FSYY1028 pKa = 11.19VPTSFKK1034 pKa = 9.67TANVSPGLCISGDD1047 pKa = 3.15RR1048 pKa = 11.84GLAPKK1053 pKa = 10.3AGYY1056 pKa = 8.93FVQDD1060 pKa = 3.0NGEE1063 pKa = 4.07WKK1065 pKa = 8.91FTGSNYY1071 pKa = 10.17YY1072 pKa = 9.98YY1073 pKa = 9.83PEE1075 pKa = 5.68PITDD1079 pKa = 3.59KK1080 pKa = 10.85NSVVMISCAVNYY1092 pKa = 7.8TKK1094 pKa = 10.77APEE1097 pKa = 4.02VFLNNSIPNLPDD1109 pKa = 3.66FKK1111 pKa = 11.37EE1112 pKa = 4.4EE1113 pKa = 3.64LDD1115 pKa = 3.46KK1116 pKa = 11.03WFKK1119 pKa = 10.55NQTSIAPDD1127 pKa = 3.69LSLDD1131 pKa = 3.81FEE1133 pKa = 4.58KK1134 pKa = 11.4LNVTFLDD1141 pKa = 3.44LTYY1144 pKa = 11.03EE1145 pKa = 4.04MNRR1148 pKa = 11.84IQDD1151 pKa = 4.14AIKK1154 pKa = 10.34KK1155 pKa = 10.05LNEE1158 pKa = 3.82SYY1160 pKa = 11.14INLKK1164 pKa = 10.23EE1165 pKa = 3.97VGTYY1169 pKa = 9.32EE1170 pKa = 5.21MYY1172 pKa = 11.04VKK1174 pKa = 9.96WPWYY1178 pKa = 8.24VWLLIGLAGVAVCVLLFFICCCTGCGSCCFRR1209 pKa = 11.84KK1210 pKa = 10.28CGSCCDD1216 pKa = 3.87EE1217 pKa = 4.38YY1218 pKa = 11.48GGHH1221 pKa = 6.11QDD1223 pKa = 4.52SIVIHH1228 pKa = 6.54NISAHH1233 pKa = 5.82EE1234 pKa = 4.06DD1235 pKa = 3.09

Molecular weight: 136.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7AB47|I7AB47_9BETC Hemagglutinin-esterase OS=Murine coronavirus OX=694005 GN=HE PE=3 SV=1
MM1 pKa = 7.46ALIGPKK7 pKa = 7.57TTIAAVFIGPFLVACMLGIGLVYY30 pKa = 10.81LLQLQVQIFHH40 pKa = 6.45VKK42 pKa = 9.07DD43 pKa = 3.89TIRR46 pKa = 11.84VTGKK50 pKa = 9.2PATVSYY56 pKa = 7.5TTSTPVTPVATTLDD70 pKa = 3.53GTTYY74 pKa = 10.5TLIRR78 pKa = 11.84PTSSYY83 pKa = 9.16TRR85 pKa = 11.84VYY87 pKa = 10.18LGSSRR92 pKa = 11.84GFDD95 pKa = 2.9TSTFGPKK102 pKa = 9.08TLDD105 pKa = 3.78YY106 pKa = 9.39ITSSKK111 pKa = 10.15PHH113 pKa = 6.34LNSGRR118 pKa = 11.84PYY120 pKa = 10.28TLRR123 pKa = 11.84HH124 pKa = 5.44LPKK127 pKa = 10.79YY128 pKa = 6.04MTPPATWRR136 pKa = 11.84FGLL139 pKa = 4.06

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

14344

88

7180

1593.8

177.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.965 ± 0.17

3.758 ± 0.249

5.556 ± 0.333

4.037 ± 0.23

5.243 ± 0.109

6.177 ± 0.274

1.645 ± 0.158

4.19 ± 0.415

5.779 ± 0.38

9.126 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.192

4.971 ± 0.596

3.841 ± 0.293

3.437 ± 0.297

3.681 ± 0.251

7.139 ± 0.15

5.752 ± 0.345

10.213 ± 0.815

1.339 ± 0.108

4.769 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski