Gracilibacillus ureilyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Gracilibacillus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3985 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9MYV4|A0A1H9MYV4_9BACI PadR family transcriptional regulator regulatory protein PadR OS=Gracilibacillus ureilyticus OX=531814 GN=SAMN04487944_102232 PE=4 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84KK3 pKa = 9.65SYY5 pKa = 11.3ALLVMLLSAMLVLSACAGGGSSSGDD30 pKa = 3.05KK31 pKa = 11.29GEE33 pKa = 4.13AAEE36 pKa = 5.49DD37 pKa = 3.82GTKK40 pKa = 10.57EE41 pKa = 3.77LTFMFRR47 pKa = 11.84GGTAEE52 pKa = 3.76EE53 pKa = 3.94EE54 pKa = 4.56AYY56 pKa = 10.55QKK58 pKa = 11.1AIDD61 pKa = 3.88QFEE64 pKa = 4.3ADD66 pKa = 3.61NEE68 pKa = 4.54GVKK71 pKa = 10.96VNMIVTAADD80 pKa = 3.39QYY82 pKa = 9.23STKK85 pKa = 10.52LKK87 pKa = 10.75AAITGNQVPDD97 pKa = 3.26VFYY100 pKa = 10.64IEE102 pKa = 4.66PGQLRR107 pKa = 11.84SYY109 pKa = 9.16VHH111 pKa = 6.79SNILLDD117 pKa = 3.43LTEE120 pKa = 4.56YY121 pKa = 10.61IEE123 pKa = 4.81NNDD126 pKa = 4.79NINLDD131 pKa = 4.24NIWQYY136 pKa = 11.69GVDD139 pKa = 3.35SYY141 pKa = 11.82RR142 pKa = 11.84FDD144 pKa = 5.54GEE146 pKa = 4.45TQGQGAIYY154 pKa = 10.49AMPKK158 pKa = 10.31DD159 pKa = 3.77VGPFSLGYY167 pKa = 9.33NKK169 pKa = 10.05TMFEE173 pKa = 4.13EE174 pKa = 5.05AGLEE178 pKa = 4.07LPDD181 pKa = 4.39EE182 pKa = 4.83DD183 pKa = 6.2VPMTWDD189 pKa = 3.32EE190 pKa = 4.64FIEE193 pKa = 4.32ANQALTKK200 pKa = 10.3DD201 pKa = 3.35TDD203 pKa = 3.71GDD205 pKa = 4.31GEE207 pKa = 4.07IDD209 pKa = 3.62QYY211 pKa = 11.44GTGFNVNWVLQSFVWSNGGSFIDD234 pKa = 3.67EE235 pKa = 4.43TQTKK239 pKa = 7.54VTVDD243 pKa = 3.26TPEE246 pKa = 3.9FAEE249 pKa = 4.16ALQWFADD256 pKa = 3.64HH257 pKa = 6.3QNVYY261 pKa = 10.19EE262 pKa = 4.08ITPSIEE268 pKa = 3.9DD269 pKa = 3.57AQTLDD274 pKa = 3.24TYY276 pKa = 9.51QRR278 pKa = 11.84WMQGQLAFFPVAPWDD293 pKa = 3.5LATFDD298 pKa = 3.97TEE300 pKa = 5.61LDD302 pKa = 3.48FEE304 pKa = 5.08YY305 pKa = 11.11DD306 pKa = 3.93VIPYY310 pKa = 8.78PAGKK314 pKa = 8.25TGEE317 pKa = 4.16SATWIGTLGIGVAQSSEE334 pKa = 4.13YY335 pKa = 10.25PEE337 pKa = 4.58LATEE341 pKa = 4.21LVYY344 pKa = 11.22YY345 pKa = 8.54LTASEE350 pKa = 4.84EE351 pKa = 4.39GQQALVDD358 pKa = 3.86ARR360 pKa = 11.84IQIPNLIDD368 pKa = 3.53MAQEE372 pKa = 3.77WAADD376 pKa = 3.65EE377 pKa = 4.72SMAPANKK384 pKa = 9.86EE385 pKa = 3.62EE386 pKa = 3.86YY387 pKa = 10.32LQIIEE392 pKa = 4.57DD393 pKa = 3.81YY394 pKa = 11.21GRR396 pKa = 11.84ALPANNTYY404 pKa = 9.78NAEE407 pKa = 3.86WYY409 pKa = 9.87DD410 pKa = 3.75YY411 pKa = 10.93FFTNVQPVLDD421 pKa = 4.12GDD423 pKa = 3.7ITAAEE428 pKa = 4.48YY429 pKa = 10.98VEE431 pKa = 4.39QVQPKK436 pKa = 6.83MQEE439 pKa = 3.95YY440 pKa = 10.31LDD442 pKa = 3.67NARR445 pKa = 11.84EE446 pKa = 4.07LEE448 pKa = 4.29EE449 pKa = 4.34QSKK452 pKa = 8.56QQ453 pKa = 3.31

Molecular weight: 50.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9V064|A0A1H9V064_9BACI tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Gracilibacillus ureilyticus OX=531814 GN=mnmG PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.75VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.2KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3985

0

3985

1176553

11

2311

295.2

33.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.62 ± 0.04

0.65 ± 0.012

5.403 ± 0.033

7.84 ± 0.052

4.595 ± 0.037

6.497 ± 0.038

2.098 ± 0.022

8.258 ± 0.044

6.632 ± 0.04

9.56 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.02

4.812 ± 0.031

3.445 ± 0.021

3.958 ± 0.029

3.808 ± 0.029

6.024 ± 0.03

5.432 ± 0.024

6.749 ± 0.033

1.119 ± 0.017

3.752 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski