Pelagibacter phage HTVC200P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Votkovvirus; unclassified Votkovvirus

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NTV0|A0A4Y1NTV0_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC200P OX=2283023 GN=P200_gp041 PE=4 SV=1
MM1 pKa = 7.57ANSFLEE7 pKa = 4.17YY8 pKa = 9.72TGNGSTTAFSITFDD22 pKa = 3.78YY23 pKa = 11.26LDD25 pKa = 4.44ASHH28 pKa = 7.37IACTVDD34 pKa = 3.62GVSTSFTLSNGGTTATLAAAPANGAAIRR62 pKa = 11.84FTRR65 pKa = 11.84TTSQATRR72 pKa = 11.84LTDD75 pKa = 3.51YY76 pKa = 10.99VAGSVLTEE84 pKa = 3.99TDD86 pKa = 3.77LDD88 pKa = 4.05TDD90 pKa = 3.98SKK92 pKa = 11.27QAFFMGQEE100 pKa = 4.19GLDD103 pKa = 3.76TIGTKK108 pKa = 9.4MGQSISTFQFDD119 pKa = 4.16ALNKK123 pKa = 10.07RR124 pKa = 11.84ITNVADD130 pKa = 3.26PTSAQDD136 pKa = 3.52AATKK140 pKa = 10.59NYY142 pKa = 10.63LEE144 pKa = 4.22NTWLSSTDD152 pKa = 3.32KK153 pKa = 10.82ATLNSVNANIANINAVNSNSSNINSAVSNATNINTVATNIGSVNTVAADD202 pKa = 3.23ITKK205 pKa = 9.97VVAVANDD212 pKa = 3.39LAEE215 pKa = 4.27AVSEE219 pKa = 4.51VEE221 pKa = 4.71TVADD225 pKa = 4.55DD226 pKa = 4.36LNEE229 pKa = 4.13ATSEE233 pKa = 3.84IDD235 pKa = 3.32TVANAITNVDD245 pKa = 3.25NVGNNIANVNSVASNATNINAVAGNATNINAVNSNSANINTVAGQNANITTLAGINANITTVAGIAANVTSVAGNEE321 pKa = 4.16TNINAVNSNSANINTVAGANSNITSVAGSIANVNTVATNLASVNSFANTYY371 pKa = 9.44RR372 pKa = 11.84ISSSAPTTSLDD383 pKa = 3.77VGDD386 pKa = 5.38LYY388 pKa = 11.5FDD390 pKa = 3.53TTANEE395 pKa = 3.92LKK397 pKa = 10.5VYY399 pKa = 10.44KK400 pKa = 10.46SSGWAAAGSTVNGTAQRR417 pKa = 11.84YY418 pKa = 5.38TYY420 pKa = 10.27NITGTPTTVSGSDD433 pKa = 3.32ANGNTLAYY441 pKa = 9.86DD442 pKa = 3.68AGYY445 pKa = 11.18ADD447 pKa = 4.47VYY449 pKa = 11.31LNGIRR454 pKa = 11.84MSSADD459 pKa = 3.2ITITSGTSVVFASALANGDD478 pKa = 3.63VVDD481 pKa = 4.41VVAYY485 pKa = 7.85GTFNVASINAANIDD499 pKa = 3.72AGTLNNARR507 pKa = 11.84LPSTISDD514 pKa = 3.45KK515 pKa = 10.93TIEE518 pKa = 4.22ATALTAKK525 pKa = 10.53GDD527 pKa = 3.63GSSADD532 pKa = 3.46GKK534 pKa = 9.41ITLNCSQNSHH544 pKa = 5.34GVAIQSPAHH553 pKa = 6.14SAGQSYY559 pKa = 7.71TLILPTSVGSNGQVLATAGSNTNQLTWVDD588 pKa = 3.61AQEE591 pKa = 4.39TKK593 pKa = 9.39PTVANVSQTIAPATATTITITGTNFVSIPHH623 pKa = 5.59VEE625 pKa = 4.27FVKK628 pKa = 10.31TDD630 pKa = 3.49GSVTIANTVSFTSSTSLSVNVTLASGNYY658 pKa = 7.4YY659 pKa = 10.35VRR661 pKa = 11.84IEE663 pKa = 4.31NPDD666 pKa = 3.26GNAGRR671 pKa = 11.84STQNIITASTAPSFTTSAGSLGTIAGNFSGTVATIAGTSDD711 pKa = 3.16SAITFSEE718 pKa = 4.55TTSVLTTANVTLNTSTGALTTTDD741 pKa = 4.03FGGSSTTPTTYY752 pKa = 11.06NFTIRR757 pKa = 11.84ITDD760 pKa = 3.64AEE762 pKa = 4.46GQTADD767 pKa = 4.08RR768 pKa = 11.84NFSLTSSFGATGGGQFNN785 pKa = 4.04

Molecular weight: 79.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NTT2|A0A4Y1NTT2_9CAUD Tail tubular protein B OS=Pelagibacter phage HTVC200P OX=2283023 GN=P200_gp029 PE=4 SV=1
MM1 pKa = 6.7VAKK4 pKa = 10.21RR5 pKa = 11.84YY6 pKa = 8.29QSPSGGLNARR16 pKa = 11.84GRR18 pKa = 11.84KK19 pKa = 9.07KK20 pKa = 10.61FGVKK24 pKa = 10.34APVKK28 pKa = 10.24KK29 pKa = 9.29GTNSRR34 pKa = 11.84RR35 pKa = 11.84VSFAARR41 pKa = 11.84FSGMKK46 pKa = 10.51GPMKK50 pKa = 10.65KK51 pKa = 9.93NGKK54 pKa = 5.21PTRR57 pKa = 11.84KK58 pKa = 9.84ALALKK63 pKa = 9.71KK64 pKa = 9.66WGFGSVAAARR74 pKa = 11.84KK75 pKa = 8.33FAAKK79 pKa = 9.83NKK81 pKa = 10.03KK82 pKa = 8.96SS83 pKa = 3.32

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13289

40

1201

250.7

27.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.773 ± 0.467

0.903 ± 0.142

5.711 ± 0.234

5.584 ± 0.424

3.981 ± 0.185

7.668 ± 0.798

1.46 ± 0.199

6.569 ± 0.189

8.15 ± 0.729

7.668 ± 0.43

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.165

6.532 ± 0.361

3.07 ± 0.232

3.544 ± 0.206

3.913 ± 0.297

7.442 ± 0.481

7.457 ± 0.674

5.599 ± 0.248

1.204 ± 0.148

3.785 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski