Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1XJA3|B1XJA3_SYNP2 Uncharacterized protein OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=SYNPCC7002_A2290 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 10.46QSATRR7 pKa = 11.84LRR9 pKa = 11.84TLSLGLAGLTLTAALAACNTTQTPTEE35 pKa = 4.28GTGTTDD41 pKa = 3.61APADD45 pKa = 3.86SASGLSGDD53 pKa = 3.94VLIDD57 pKa = 3.61GSSTVFPITEE67 pKa = 3.69AMAEE71 pKa = 4.17EE72 pKa = 4.18FQIANPDD79 pKa = 3.13TRR81 pKa = 11.84VTVGVSGTGGGFSKK95 pKa = 10.61FCAGEE100 pKa = 3.98TDD102 pKa = 2.87ISNASRR108 pKa = 11.84PISTEE113 pKa = 3.64EE114 pKa = 4.33IEE116 pKa = 4.46ACEE119 pKa = 3.85QAGIEE124 pKa = 4.25YY125 pKa = 10.2IEE127 pKa = 4.71IPVAYY132 pKa = 9.65DD133 pKa = 4.2AISVVINPEE142 pKa = 3.97NDD144 pKa = 2.69WATCLTTEE152 pKa = 4.43EE153 pKa = 4.85LASIWSPDD161 pKa = 3.24SQINNWSQVRR171 pKa = 11.84EE172 pKa = 4.28GFPDD176 pKa = 3.89APLTLYY182 pKa = 11.07GPGTDD187 pKa = 4.59SGTFDD192 pKa = 4.07YY193 pKa = 8.83FTDD196 pKa = 5.39AINGEE201 pKa = 4.39EE202 pKa = 4.35GASRR206 pKa = 11.84GDD208 pKa = 3.59YY209 pKa = 8.26TASEE213 pKa = 4.57DD214 pKa = 4.55DD215 pKa = 3.69NVVVQGVVNDD225 pKa = 4.46PNAMGYY231 pKa = 10.26FGLAYY236 pKa = 10.57YY237 pKa = 10.05EE238 pKa = 4.39EE239 pKa = 4.61NADD242 pKa = 4.03NLNAAAIDD250 pKa = 4.91DD251 pKa = 4.55GDD253 pKa = 3.97PSNGDD258 pKa = 3.23GCVAPSTATVDD269 pKa = 3.44DD270 pKa = 4.01STYY273 pKa = 10.86QPLARR278 pKa = 11.84PIFIYY283 pKa = 10.81VSAASLDD290 pKa = 3.85EE291 pKa = 4.4KK292 pKa = 10.74PQVQAFMDD300 pKa = 4.99FYY302 pKa = 10.41TAEE305 pKa = 4.33EE306 pKa = 4.05NSSLVSEE313 pKa = 4.54VGYY316 pKa = 10.58VALNSEE322 pKa = 5.09IYY324 pKa = 10.78AKK326 pKa = 10.58AQEE329 pKa = 4.06RR330 pKa = 11.84LAARR334 pKa = 11.84TTGSIFNGGSTVGVRR349 pKa = 11.84LGEE352 pKa = 4.19VLL354 pKa = 4.05

Molecular weight: 37.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B1XJH8|RF1_SYNP2 Peptide chain release factor 1 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=prfA PE=3 SV=1
MM1 pKa = 7.55TKK3 pKa = 8.94RR4 pKa = 11.84TLGGTVRR11 pKa = 11.84KK12 pKa = 9.36QKK14 pKa = 9.22RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 7.11TGQNVIRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.08KK38 pKa = 9.6GRR40 pKa = 11.84HH41 pKa = 5.24RR42 pKa = 11.84LTVV45 pKa = 3.07

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3179

1

3180

988541

30

2720

310.9

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.982 ± 0.047

1.019 ± 0.014

5.179 ± 0.033

6.14 ± 0.043

4.064 ± 0.034

7.022 ± 0.043

1.969 ± 0.025

6.283 ± 0.037

4.17 ± 0.038

11.276 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.021

3.739 ± 0.036

5.124 ± 0.034

5.753 ± 0.046

5.256 ± 0.031

5.474 ± 0.031

5.804 ± 0.035

6.382 ± 0.037

1.489 ± 0.022

2.936 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski