Human immunodeficiency virus type 1 group M subtype B (isolate ARV2/SF2) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:B

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P05952|VPR_HV1A2 Protein Vpr OS=Human immunodeficiency virus type 1 group M subtype B (isolate ARV2/SF2) OX=11685 GN=vpr PE=3 SV=1
MM1 pKa = 7.57QSLQILAIVSLVVVAIIAIVVWTIVLIEE29 pKa = 3.9YY30 pKa = 10.39RR31 pKa = 11.84KK32 pKa = 9.61ILRR35 pKa = 11.84QRR37 pKa = 11.84KK38 pKa = 8.35IDD40 pKa = 3.59RR41 pKa = 11.84LFDD44 pKa = 4.87RR45 pKa = 11.84IRR47 pKa = 11.84EE48 pKa = 4.05KK49 pKa = 11.33AEE51 pKa = 3.98DD52 pKa = 3.59SGNEE56 pKa = 3.86SEE58 pKa = 5.32RR59 pKa = 11.84DD60 pKa = 3.66QEE62 pKa = 4.13EE63 pKa = 4.17LSALVEE69 pKa = 4.27MGHH72 pKa = 6.91LAPWDD77 pKa = 3.7VDD79 pKa = 3.88DD80 pKa = 5.48LL81 pKa = 4.6

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P05949|VPU_HV1A2 Protein Vpu OS=Human immunodeficiency virus type 1 group M subtype B (isolate ARV2/SF2) OX=11685 GN=vpu PE=3 SV=2
MM1 pKa = 7.87AGRR4 pKa = 11.84SGDD7 pKa = 3.5SDD9 pKa = 4.04EE10 pKa = 4.35EE11 pKa = 4.03LLRR14 pKa = 11.84TVRR17 pKa = 11.84LIKK20 pKa = 10.44LLYY23 pKa = 9.8QSNPPPSPEE32 pKa = 3.55GTRR35 pKa = 11.84QARR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84WRR46 pKa = 11.84EE47 pKa = 3.07RR48 pKa = 11.84QRR50 pKa = 11.84QIRR53 pKa = 11.84SISGWILSTYY63 pKa = 9.9LGRR66 pKa = 11.84SAEE69 pKa = 4.15PVPLQLPPLEE79 pKa = 5.3RR80 pKa = 11.84LTLDD84 pKa = 3.9CSEE87 pKa = 4.81DD88 pKa = 3.79CGNSGAQGVGSPQILVEE105 pKa = 4.47SPAVLDD111 pKa = 4.04SGTKK115 pKa = 8.74EE116 pKa = 3.73

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3663

72

1437

366.3

41.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.579 ± 0.38

2.02 ± 0.375

3.877 ± 0.298

7.18 ± 0.534

2.484 ± 0.161

7.153 ± 0.257

2.266 ± 0.397

6.334 ± 0.651

6.989 ± 0.971

8.217 ± 0.561

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.229

4.696 ± 0.644

5.269 ± 0.579

5.924 ± 0.411

6.334 ± 0.844

5.242 ± 0.516

5.815 ± 0.451

6.006 ± 0.52

2.757 ± 0.276

2.675 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski