Wuhan pillworm virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN17|A0A1L3KN17_9VIRU Nucleocapsid protein OS=Wuhan pillworm virus 2 OX=1923745 PE=4 SV=1
MM1 pKa = 8.29DD2 pKa = 3.89STTVITKK9 pKa = 10.78KK10 pKa = 9.74MMKK13 pKa = 8.72TLRR16 pKa = 11.84GLDD19 pKa = 3.39KK20 pKa = 10.64RR21 pKa = 11.84LKK23 pKa = 10.89EE24 pKa = 3.95EE25 pKa = 3.93DD26 pKa = 3.09QKK28 pKa = 11.9YY29 pKa = 10.07SDD31 pKa = 3.8NVEE34 pKa = 4.33SQGSGPSFLTEE45 pKa = 3.57KK46 pKa = 9.07TQEE49 pKa = 4.12FFSGPVTSVAEE60 pKa = 4.06QPNEE64 pKa = 4.01VVVEE68 pKa = 4.2DD69 pKa = 4.69DD70 pKa = 3.58NEE72 pKa = 4.36SRR74 pKa = 11.84SSEE77 pKa = 4.01DD78 pKa = 3.09QEE80 pKa = 4.14EE81 pKa = 4.16FSIIPRR87 pKa = 11.84RR88 pKa = 11.84IIIDD92 pKa = 3.07VSRR95 pKa = 11.84VEE97 pKa = 3.79RR98 pKa = 11.84RR99 pKa = 11.84LVNWLLSTLDD109 pKa = 3.77FFHH112 pKa = 6.98IPHH115 pKa = 6.65RR116 pKa = 11.84EE117 pKa = 3.59EE118 pKa = 4.44GEE120 pKa = 3.84RR121 pKa = 11.84DD122 pKa = 3.64NPGQSYY128 pKa = 11.23DD129 pKa = 4.35DD130 pKa = 4.48EE131 pKa = 4.84IKK133 pKa = 10.64SFRR136 pKa = 11.84RR137 pKa = 11.84NEE139 pKa = 3.91ATSTEE144 pKa = 4.12IKK146 pKa = 10.28DD147 pKa = 4.49DD148 pKa = 3.34SWGDD152 pKa = 3.41YY153 pKa = 10.03QEE155 pKa = 5.11NIEE158 pKa = 4.18EE159 pKa = 4.36KK160 pKa = 10.35EE161 pKa = 4.32SKK163 pKa = 10.65KK164 pKa = 10.92LINNKK169 pKa = 9.54YY170 pKa = 10.59DD171 pKa = 4.15PDD173 pKa = 3.64LMRR176 pKa = 11.84FTMKK180 pKa = 10.14GRR182 pKa = 11.84KK183 pKa = 7.1GQKK186 pKa = 9.57ARR188 pKa = 11.84IIFMSDD194 pKa = 2.12IFDD197 pKa = 4.1NDD199 pKa = 4.47FSPLWNCKK207 pKa = 7.83TLTDD211 pKa = 3.74VKK213 pKa = 11.17NKK215 pKa = 9.9IKK217 pKa = 9.81EE218 pKa = 4.1TGFYY222 pKa = 9.99ARR224 pKa = 11.84YY225 pKa = 8.54YY226 pKa = 10.9RR227 pKa = 11.84MYY229 pKa = 10.71NLEE232 pKa = 4.31DD233 pKa = 3.74YY234 pKa = 9.97EE235 pKa = 5.18IKK237 pKa = 10.57FDD239 pKa = 3.83TQQ241 pKa = 2.92

Molecular weight: 28.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNC8|A0A1L3KNC8_9VIRU Putative matrix protein OS=Wuhan pillworm virus 2 OX=1923745 PE=4 SV=1
MM1 pKa = 7.33SLSKK5 pKa = 10.32FRR7 pKa = 11.84AILSRR12 pKa = 11.84STEE15 pKa = 4.18DD16 pKa = 4.27APPPYY21 pKa = 10.42NPGWSLEE28 pKa = 4.12KK29 pKa = 10.1VVEE32 pKa = 4.13RR33 pKa = 11.84ARR35 pKa = 11.84IDD37 pKa = 3.11TSYY40 pKa = 11.18QITSSLRR47 pKa = 11.84IVSNRR52 pKa = 11.84EE53 pKa = 3.5MTEE56 pKa = 3.42HH57 pKa = 6.61HH58 pKa = 6.71GEE60 pKa = 3.94MMLEE64 pKa = 3.93KK65 pKa = 10.49LKK67 pKa = 10.47EE68 pKa = 4.11YY69 pKa = 10.77YY70 pKa = 10.15SGSVRR75 pKa = 11.84MRR77 pKa = 11.84DD78 pKa = 3.55FYY80 pKa = 11.39MLIFLAAFNSVGQGKK95 pKa = 8.42VQGFNTCWEE104 pKa = 4.29VNFDD108 pKa = 3.54GVVRR112 pKa = 11.84LGTDD116 pKa = 3.49KK117 pKa = 10.85DD118 pKa = 3.68PPMIPIQPGTISGEE132 pKa = 4.06VCANGIKK139 pKa = 10.46ARR141 pKa = 11.84FNFVSTIHH149 pKa = 6.83RR150 pKa = 11.84SNAVGLPLSVFIAGRR165 pKa = 11.84NKK167 pKa = 9.66VALGTSLYY175 pKa = 10.13RR176 pKa = 11.84ASLLDD181 pKa = 3.48INISVIDD188 pKa = 3.94DD189 pKa = 4.32EE190 pKa = 4.84IVVKK194 pKa = 10.81II195 pKa = 4.18

Molecular weight: 21.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3917

195

2144

652.8

74.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.161 ± 0.677

1.659 ± 0.455

5.897 ± 0.727

6.28 ± 0.489

4.57 ± 0.167

6.255 ± 0.232

2.247 ± 0.209

7.25 ± 0.362

6.229 ± 0.564

9.701 ± 0.724

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.205

3.881 ± 0.275

4.851 ± 0.673

3.064 ± 0.11

5.744 ± 0.325

7.863 ± 0.526

6.587 ± 0.451

5.744 ± 0.339

2.221 ± 0.338

3.625 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski