Humitalea rosea

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Humitalea

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W7IT37|A0A2W7IT37_9PROT Cell shape-determining protein MreB OS=Humitalea rosea OX=990373 GN=mreB PE=3 SV=1
MM1 pKa = 7.63ARR3 pKa = 11.84FTLTEE8 pKa = 3.89NALITPQVPGFARR21 pKa = 11.84LDD23 pKa = 3.63LLATITLPNVSDD35 pKa = 3.87AYY37 pKa = 10.96VYY39 pKa = 11.35GDD41 pKa = 4.01FRR43 pKa = 11.84ASGQAAYY50 pKa = 10.22SVEE53 pKa = 3.79FLGFGLTAANGRR65 pKa = 11.84LSDD68 pKa = 3.84GTITALKK75 pKa = 8.32YY76 pKa = 8.57TLSGIQVFYY85 pKa = 11.28LDD87 pKa = 5.13LLQLPVATFNAYY99 pKa = 9.17IDD101 pKa = 3.99SRR103 pKa = 11.84DD104 pKa = 3.44EE105 pKa = 4.46AGFATYY111 pKa = 10.53LFSGNDD117 pKa = 3.33EE118 pKa = 4.6LYY120 pKa = 8.49GTSRR124 pKa = 11.84ADD126 pKa = 3.42LLTGYY131 pKa = 10.54GGDD134 pKa = 3.99DD135 pKa = 3.72VIGGKK140 pKa = 9.98DD141 pKa = 3.74GNDD144 pKa = 3.18TLAGGEE150 pKa = 4.51GNDD153 pKa = 3.78TLYY156 pKa = 11.48GEE158 pKa = 4.98ADD160 pKa = 3.3NDD162 pKa = 3.66VLYY165 pKa = 10.81GAGGADD171 pKa = 3.52EE172 pKa = 4.45VHH174 pKa = 6.66GGTGNDD180 pKa = 4.38FIYY183 pKa = 10.61LGEE186 pKa = 4.31GADD189 pKa = 3.58NGFGEE194 pKa = 5.01AGNDD198 pKa = 3.74TIFGEE203 pKa = 4.54GGNDD207 pKa = 3.32IIAGGVEE214 pKa = 3.77NDD216 pKa = 4.36YY217 pKa = 11.32IDD219 pKa = 5.16GGNGDD224 pKa = 5.35DD225 pKa = 4.31NLQGEE230 pKa = 4.86AGNDD234 pKa = 3.69TLRR237 pKa = 11.84GGAGNDD243 pKa = 3.83SISGGVEE250 pKa = 3.43EE251 pKa = 5.07DD252 pKa = 4.04LVYY255 pKa = 11.23GDD257 pKa = 5.14AGNDD261 pKa = 3.64SIYY264 pKa = 10.74LDD266 pKa = 3.91SGQDD270 pKa = 3.15TAYY273 pKa = 10.64GGTGDD278 pKa = 4.0DD279 pKa = 4.53TIFGDD284 pKa = 3.54TGDD287 pKa = 4.23DD288 pKa = 3.65VLVGEE293 pKa = 5.59DD294 pKa = 4.73GDD296 pKa = 4.14DD297 pKa = 3.59QLFGGNGNDD306 pKa = 4.16TILGGAGADD315 pKa = 3.22QLFGGEE321 pKa = 4.72NLDD324 pKa = 4.83LLYY327 pKa = 11.24GDD329 pKa = 4.73IGNDD333 pKa = 3.18TLYY336 pKa = 11.39GEE338 pKa = 5.14GSFDD342 pKa = 3.5TAGVSTLWRR351 pKa = 11.84QNTLTGTVGNFTLTGPEE368 pKa = 4.27GVDD371 pKa = 2.94TLYY374 pKa = 10.91SIEE377 pKa = 4.75SIRR380 pKa = 11.84SLDD383 pKa = 3.08GWLEE387 pKa = 3.91LDD389 pKa = 3.28GGGPVSQVARR399 pKa = 11.84LYY401 pKa = 10.92LATLGRR407 pKa = 11.84ASDD410 pKa = 3.79ATGLGYY416 pKa = 10.57YY417 pKa = 9.4VAAIEE422 pKa = 5.67AGTQTLNDD430 pKa = 3.26ISAGLVASAEE440 pKa = 3.96FGARR444 pKa = 11.84YY445 pKa = 9.47GNPGNSDD452 pKa = 3.85FVTLLYY458 pKa = 11.19ANVLNRR464 pKa = 11.84APDD467 pKa = 3.6SGGLAYY473 pKa = 10.43YY474 pKa = 9.43VARR477 pKa = 11.84LDD479 pKa = 4.07AGEE482 pKa = 3.94QRR484 pKa = 11.84STIVLDD490 pKa = 4.38FSEE493 pKa = 4.71SDD495 pKa = 3.29EE496 pKa = 4.6FIYY499 pKa = 10.61RR500 pKa = 11.84AGYY503 pKa = 8.66PGEE506 pKa = 4.36GGIFAPDD513 pKa = 3.68PVAVDD518 pKa = 3.2VVRR521 pKa = 11.84YY522 pKa = 9.64YY523 pKa = 10.24EE524 pKa = 4.27TVLDD528 pKa = 4.29RR529 pKa = 11.84LPDD532 pKa = 4.01AGGAAYY538 pKa = 8.75WIQLRR543 pKa = 11.84HH544 pKa = 6.08GGLSLANMGAAFTGSAEE561 pKa = 3.92FEE563 pKa = 4.22SRR565 pKa = 11.84YY566 pKa = 10.16GALSNSGFVEE576 pKa = 3.83QLYY579 pKa = 11.23LNALDD584 pKa = 4.13RR585 pKa = 11.84AGDD588 pKa = 3.77SGGIAYY594 pKa = 7.21WTAEE598 pKa = 4.24LDD600 pKa = 3.27HH601 pKa = 6.68GALSRR606 pKa = 11.84AGVADD611 pKa = 3.69SFAFSAEE618 pKa = 3.8MTAKK622 pKa = 10.39VEE624 pKa = 3.99PLVDD628 pKa = 3.68GGITFAA634 pKa = 5.51

Molecular weight: 65.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W7I6H0|A0A2W7I6H0_9PROT Formate dehydrogenase gamma subunit OS=Humitalea rosea OX=990373 GN=C8P66_11840 PE=3 SV=1
GG1 pKa = 7.01KK2 pKa = 9.51RR3 pKa = 11.84HH4 pKa = 5.45HH5 pKa = 6.27QAVIALARR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VNVLWAVVQNRR26 pKa = 11.84TPFQTRR32 pKa = 11.84FKK34 pKa = 10.09MAAA37 pKa = 3.11

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4650

0

4650

1537676

25

4046

330.7

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.091 ± 0.064

0.853 ± 0.011

5.115 ± 0.024

5.145 ± 0.03

3.311 ± 0.02

9.335 ± 0.039

1.976 ± 0.016

4.384 ± 0.021

1.864 ± 0.023

11.071 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.017

1.991 ± 0.016

6.255 ± 0.032

2.867 ± 0.02

7.986 ± 0.033

4.502 ± 0.022

5.178 ± 0.034

7.417 ± 0.03

1.453 ± 0.015

1.73 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski