Halorubrum hochstenium ATCC 700873

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; Halorubrum hochstenium

Average proteome isoelectric point is 4.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2931 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0FG02|M0FG02_9EURY Nucleotide pyrophosphohydrolase OS=Halorubrum hochstenium ATCC 700873 OX=1227481 GN=C467_04515 PE=4 SV=1
MM1 pKa = 7.14VGAASAAPGNGGGPDD16 pKa = 3.05RR17 pKa = 11.84RR18 pKa = 11.84EE19 pKa = 4.07YY20 pKa = 10.56IVGVSATEE28 pKa = 4.44DD29 pKa = 3.5DD30 pKa = 3.71PKK32 pKa = 11.41GYY34 pKa = 10.5VKK36 pKa = 10.33QHH38 pKa = 5.71LKK40 pKa = 10.91GNEE43 pKa = 3.87EE44 pKa = 4.07VLSSNEE50 pKa = 4.05TLNTTVVGLPEE61 pKa = 4.14NAAEE65 pKa = 4.15PAHH68 pKa = 6.07EE69 pKa = 4.36NFRR72 pKa = 11.84EE73 pKa = 3.99RR74 pKa = 11.84QKK76 pKa = 10.97RR77 pKa = 11.84ADD79 pKa = 3.14AVKK82 pKa = 10.01YY83 pKa = 9.71VEE85 pKa = 4.18EE86 pKa = 4.19NVRR89 pKa = 11.84LTAQLAPNDD98 pKa = 4.09PQYY101 pKa = 11.64GDD103 pKa = 3.55QYY105 pKa = 11.89ADD107 pKa = 3.44QQVNAPDD114 pKa = 3.12AWEE117 pKa = 4.14EE118 pKa = 4.27TFGDD122 pKa = 3.83ANVTIGVVDD131 pKa = 3.41QGVKK135 pKa = 10.12YY136 pKa = 10.07DD137 pKa = 4.85HH138 pKa = 7.72PDD140 pKa = 3.1LDD142 pKa = 4.37GNMDD146 pKa = 4.26DD147 pKa = 5.15AVSNYY152 pKa = 9.7GRR154 pKa = 11.84DD155 pKa = 3.65FVDD158 pKa = 5.52DD159 pKa = 5.52DD160 pKa = 4.44GDD162 pKa = 4.18PYY164 pKa = 11.21PDD166 pKa = 4.0ALSDD170 pKa = 3.91EE171 pKa = 4.57YY172 pKa = 11.17HH173 pKa = 5.76GTHH176 pKa = 6.07VSGIAAAEE184 pKa = 4.02TDD186 pKa = 3.29NGEE189 pKa = 4.15GVTGIGNSSVLSGRR203 pKa = 11.84ALSEE207 pKa = 4.08RR208 pKa = 11.84GSGSTSDD215 pKa = 3.38IADD218 pKa = 3.64AVTWAADD225 pKa = 3.29QGADD229 pKa = 4.73VINLSLGGGGYY240 pKa = 8.89TDD242 pKa = 3.24TMKK245 pKa = 10.92NAVSYY250 pKa = 8.98ATDD253 pKa = 3.07KK254 pKa = 11.2GALVVAAAGNDD265 pKa = 3.14GRR267 pKa = 11.84NSVSYY272 pKa = 8.93PAAYY276 pKa = 9.87SEE278 pKa = 4.46CLGVSALDD286 pKa = 3.78PDD288 pKa = 3.99EE289 pKa = 4.65TLASYY294 pKa = 11.3SNYY297 pKa = 10.18GNEE300 pKa = 4.48IEE302 pKa = 4.78LAAPGTNVLSTWTDD316 pKa = 2.96DD317 pKa = 3.83GYY319 pKa = 11.68EE320 pKa = 4.4KK321 pKa = 10.5ISGTSMATPVVAGVAGLTLAQYY343 pKa = 11.29DD344 pKa = 3.92LTNAEE349 pKa = 4.63LRR351 pKa = 11.84DD352 pKa = 3.96HH353 pKa = 6.93LKK355 pKa = 9.49ATAVDD360 pKa = 3.68VGLSTEE366 pKa = 4.15KK367 pKa = 10.47QGSGRR372 pKa = 11.84VDD374 pKa = 2.95AGVAVTTDD382 pKa = 3.7PEE384 pKa = 4.47SGGGGGGGGGGSGGSTSTSVTGSLSDD410 pKa = 4.19YY411 pKa = 11.38SDD413 pKa = 4.05GDD415 pKa = 3.74CLRR418 pKa = 11.84YY419 pKa = 9.97AFEE422 pKa = 4.75YY423 pKa = 10.67GDD425 pKa = 3.73PSKK428 pKa = 11.35VVLDD432 pKa = 4.14LDD434 pKa = 4.42GPSDD438 pKa = 4.53ADD440 pKa = 3.18FDD442 pKa = 5.2LYY444 pKa = 11.65ANTGVDD450 pKa = 3.74ACPTTSDD457 pKa = 3.03YY458 pKa = 11.16DD459 pKa = 3.82YY460 pKa = 11.27RR461 pKa = 11.84SWTTDD466 pKa = 2.92SQEE469 pKa = 4.32TISIDD474 pKa = 3.72GPDD477 pKa = 3.52VSTDD481 pKa = 2.73LHH483 pKa = 7.7ALVDD487 pKa = 4.21SYY489 pKa = 11.64SGSGEE494 pKa = 3.89YY495 pKa = 10.15TLTITEE501 pKa = 4.32YY502 pKa = 10.63EE503 pKa = 4.08

Molecular weight: 52.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M0F001|M0F001_9EURY Glutamate--cysteine ligase OS=Halorubrum hochstenium ATCC 700873 OX=1227481 GN=gshA PE=3 SV=1
MM1 pKa = 7.49GMKK4 pKa = 10.47NNIGAADD11 pKa = 3.69RR12 pKa = 11.84RR13 pKa = 11.84IRR15 pKa = 11.84IGLGLGSAAVGLATLGGLLGLGTTVGAVLTLLGLVLVGTALVRR58 pKa = 11.84VCLLYY63 pKa = 10.81RR64 pKa = 11.84LLGVDD69 pKa = 3.62TSGSS73 pKa = 3.38

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2931

0

2931

858121

31

1911

292.8

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.486 ± 0.077

0.635 ± 0.012

9.197 ± 0.062

8.491 ± 0.063

3.206 ± 0.031

9.281 ± 0.049

1.775 ± 0.023

3.457 ± 0.035

1.558 ± 0.025

8.531 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.565 ± 0.019

2.031 ± 0.023

4.867 ± 0.033

1.859 ± 0.024

6.988 ± 0.051

5.221 ± 0.037

5.938 ± 0.036

9.389 ± 0.045

1.046 ± 0.016

2.477 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski