Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4LF17|V4LF17_EUTSA Uncharacterized protein OS=Eutrema salsugineum OX=72664 GN=EUTSA_v10012567mg PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 5.91ADD4 pKa = 4.71EE5 pKa = 5.0ISIPSLDD12 pKa = 4.53DD13 pKa = 3.62NSDD16 pKa = 3.32INDD19 pKa = 3.41VMIVAEE25 pKa = 4.49EE26 pKa = 4.49SPDD29 pKa = 3.44SSKK32 pKa = 10.72KK33 pKa = 9.67RR34 pKa = 11.84KK35 pKa = 9.63LGAEE39 pKa = 4.07EE40 pKa = 5.07SDD42 pKa = 3.93LLPLPKK48 pKa = 10.1HH49 pKa = 5.8FCLEE53 pKa = 4.13QAAHH57 pKa = 7.29PDD59 pKa = 3.68SSCPSSDD66 pKa = 3.41IEE68 pKa = 4.76SAEE71 pKa = 3.98CSYY74 pKa = 11.89AMEE77 pKa = 4.15YY78 pKa = 9.66TKK80 pKa = 11.1NSDD83 pKa = 3.64EE84 pKa = 4.43TSSSASGTVASSYY97 pKa = 9.31MSKK100 pKa = 10.62DD101 pKa = 2.99SSYY104 pKa = 10.22STGSSSSGSGHH115 pKa = 5.91ATSSIDD121 pKa = 3.26QCCYY125 pKa = 10.13KK126 pKa = 10.78DD127 pKa = 3.28KK128 pKa = 9.27TQEE131 pKa = 4.08YY132 pKa = 10.03VEE134 pKa = 5.27DD135 pKa = 4.29LSDD138 pKa = 3.99MDD140 pKa = 4.27FLCPEE145 pKa = 4.55LAVEE149 pKa = 5.0DD150 pKa = 4.15LQEE153 pKa = 4.47LLHH156 pKa = 6.46SQDD159 pKa = 3.88SNLKK163 pKa = 9.96GSYY166 pKa = 8.91TLSSAKK172 pKa = 9.67WSVSNQDD179 pKa = 3.33SEE181 pKa = 4.49EE182 pKa = 4.12ATTKK186 pKa = 9.31PTIDD190 pKa = 3.51QEE192 pKa = 4.23FEE194 pKa = 4.55EE195 pKa = 4.76YY196 pKa = 10.61FSTLMMM202 pKa = 5.45

Molecular weight: 22.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4MG88|V4MG88_EUTSA Protein SCAR OS=Eutrema salsugineum OX=72664 GN=EUTSA_v10024197mg PE=3 SV=1
MM1 pKa = 7.55ASSKK5 pKa = 10.53VFLVASLLVALMFSSMIASSFAKK28 pKa = 9.09KK29 pKa = 8.69TIKK32 pKa = 10.23PKK34 pKa = 10.44FFRR37 pKa = 11.84HH38 pKa = 4.73HH39 pKa = 6.5HH40 pKa = 5.78FPRR43 pKa = 11.84PGFPQFPRR51 pKa = 11.84PGFPTNPMPFPQFPKK66 pKa = 10.09PGFPQFPGQGLPNNPMPFPQFPRR89 pKa = 11.84PGFPSNPTPGFPQFPGQGFPKK110 pKa = 10.24LPSPLPQIPVSPSFPPATPGSPSGNVLPLPSITAPPTLPTTPISSPP156 pKa = 3.27

Molecular weight: 16.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26426

1923

28349

11549942

13

5391

407.4

45.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.482 ± 0.014

1.847 ± 0.008

5.386 ± 0.009

6.749 ± 0.017

4.282 ± 0.009

6.401 ± 0.016

2.276 ± 0.006

5.242 ± 0.011

6.336 ± 0.013

9.542 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.005

4.338 ± 0.009

4.834 ± 0.013

3.496 ± 0.011

5.514 ± 0.01

9.126 ± 0.016

5.024 ± 0.01

6.619 ± 0.011

1.26 ± 0.005

2.807 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski