Lactococcus reticulitermitis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A224XFK8|A0A224XFK8_9LACT Imidazole glycerol phosphate synthase subunit HisH OS=Lactococcus reticulitermitis OX=2025039 GN=hisH PE=3 SV=1
MM1 pKa = 7.52TEE3 pKa = 3.85QTHH6 pKa = 6.07HH7 pKa = 7.09HH8 pKa = 6.54DD9 pKa = 3.85HH10 pKa = 5.8NHH12 pKa = 4.05EE13 pKa = 4.34HH14 pKa = 5.34EE15 pKa = 4.32QEE17 pKa = 4.52FITLIDD23 pKa = 4.21DD24 pKa = 4.22NGNEE28 pKa = 4.19TLFEE32 pKa = 4.24ILLTIDD38 pKa = 3.35GQEE41 pKa = 4.05EE42 pKa = 4.07FGKK45 pKa = 10.51NYY47 pKa = 10.4VLVVPAGAEE56 pKa = 3.67EE57 pKa = 4.49DD58 pKa = 3.77EE59 pKa = 4.49NGEE62 pKa = 4.38VEE64 pKa = 4.23IQAYY68 pKa = 10.07AYY70 pKa = 7.05TQSEE74 pKa = 4.74DD75 pKa = 3.42GTEE78 pKa = 4.34GDD80 pKa = 4.79LLPIPEE86 pKa = 4.91DD87 pKa = 4.08ANEE90 pKa = 3.96EE91 pKa = 3.54WDD93 pKa = 3.71MIEE96 pKa = 4.22EE97 pKa = 4.21VFNTFVEE104 pKa = 4.49EE105 pKa = 3.83QDD107 pKa = 3.27

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A224X2G4|A0A224X2G4_9LACT Uncharacterized protein OS=Lactococcus reticulitermitis OX=2025039 GN=RsY01_617 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.3RR10 pKa = 11.84KK11 pKa = 8.81RR12 pKa = 11.84QKK14 pKa = 8.05THH16 pKa = 5.1GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 9.01NGRR28 pKa = 11.84RR29 pKa = 11.84VVASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.24GRR39 pKa = 11.84AVLTAA44 pKa = 4.26

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2097

0

2097

642200

29

3291

306.2

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.313 ± 0.063

0.477 ± 0.014

5.987 ± 0.041

6.235 ± 0.065

4.569 ± 0.047

6.725 ± 0.059

1.835 ± 0.022

7.493 ± 0.052

6.758 ± 0.056

9.605 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.027

4.679 ± 0.042

3.362 ± 0.026

3.97 ± 0.039

3.669 ± 0.041

5.963 ± 0.045

6.444 ± 0.067

7.028 ± 0.044

0.867 ± 0.022

3.511 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski