Cronobacter phage ES2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0ZNE3|G0ZNE3_9CAUD Uncharacterized protein OS=Cronobacter phage ES2 OX=1073754 PE=4 SV=1
MM1 pKa = 7.56SEE3 pKa = 4.21LARR6 pKa = 11.84WSYY9 pKa = 9.57TGKK12 pKa = 8.69ATFWKK17 pKa = 10.31RR18 pKa = 11.84LEE20 pKa = 4.25GQNDD24 pKa = 3.49YY25 pKa = 11.6GDD27 pKa = 4.3PLGFAAPVVIDD38 pKa = 3.89CGYY41 pKa = 10.99EE42 pKa = 3.88GGLSKK47 pKa = 10.95RR48 pKa = 11.84LGDD51 pKa = 3.32IGSEE55 pKa = 3.69RR56 pKa = 11.84VIKK59 pKa = 9.37NTFWTEE65 pKa = 3.46FADD68 pKa = 4.96ADD70 pKa = 3.68MGDD73 pKa = 4.03YY74 pKa = 10.77ILIGVSTEE82 pKa = 3.82ADD84 pKa = 3.51PVQAGADD91 pKa = 3.96EE92 pKa = 4.42ILQSIRR98 pKa = 11.84DD99 pKa = 3.67EE100 pKa = 4.31DD101 pKa = 3.96TFDD104 pKa = 5.51RR105 pKa = 11.84LMDD108 pKa = 4.98DD109 pKa = 3.58YY110 pKa = 11.81AIITGGG116 pKa = 3.2

Molecular weight: 12.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0ZNC5|G0ZNC5_9CAUD Uncharacterized protein OS=Cronobacter phage ES2 OX=1073754 PE=4 SV=1
MM1 pKa = 7.52AKK3 pKa = 9.85SPRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84CKK10 pKa = 10.67NEE12 pKa = 3.3EE13 pKa = 3.82CRR15 pKa = 11.84EE16 pKa = 4.09WFHH19 pKa = 7.18PAFANQWWCGPEE31 pKa = 4.09CGAKK35 pKa = 9.64IALEE39 pKa = 3.86RR40 pKa = 11.84RR41 pKa = 11.84NRR43 pKa = 11.84EE44 pKa = 3.15RR45 pKa = 11.84DD46 pKa = 3.4KK47 pKa = 11.38ALKK50 pKa = 10.12AAEE53 pKa = 4.06KK54 pKa = 9.97KK55 pKa = 9.86RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 3.7EE60 pKa = 3.77QQQKK64 pKa = 10.79DD65 pKa = 3.2RR66 pKa = 11.84LKK68 pKa = 10.46IRR70 pKa = 11.84KK71 pKa = 8.41LALKK75 pKa = 9.56PRR77 pKa = 11.84SYY79 pKa = 9.99WIKK82 pKa = 9.98QAQQAVNAFIRR93 pKa = 11.84EE94 pKa = 3.92RR95 pKa = 11.84DD96 pKa = 3.28RR97 pKa = 11.84DD98 pKa = 4.18LPCISCGTFTSAQWDD113 pKa = 3.54AGHH116 pKa = 6.3YY117 pKa = 7.2RR118 pKa = 11.84TTAAAPQLRR127 pKa = 11.84FDD129 pKa = 4.12EE130 pKa = 4.74RR131 pKa = 11.84NIHH134 pKa = 5.37KK135 pKa = 10.44QCVVCNQHH143 pKa = 6.72KK144 pKa = 10.49SGNLVPYY151 pKa = 9.65RR152 pKa = 11.84AEE154 pKa = 4.43LIRR157 pKa = 11.84RR158 pKa = 11.84IGLSAVEE165 pKa = 4.1AVEE168 pKa = 4.41SNHH171 pKa = 5.42YY172 pKa = 8.13RR173 pKa = 11.84HH174 pKa = 6.45RR175 pKa = 11.84WTIEE179 pKa = 3.29EE180 pKa = 4.17CKK182 pKa = 10.36AIKK185 pKa = 10.41AEE187 pKa = 4.05YY188 pKa = 8.03QQKK191 pKa = 10.78LKK193 pKa = 10.79DD194 pKa = 3.65LRR196 pKa = 11.84NSRR199 pKa = 11.84EE200 pKa = 3.65EE201 pKa = 3.83AAA203 pKa = 4.68

Molecular weight: 24.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

6254

77

590

208.5

23.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.674 ± 0.479

1.279 ± 0.232

5.9 ± 0.329

6.508 ± 0.394

3.31 ± 0.224

7.147 ± 0.392

2.015 ± 0.211

5.58 ± 0.235

5.756 ± 0.33

7.931 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.216

4.717 ± 0.245

3.55 ± 0.172

4.269 ± 0.24

6.268 ± 0.404

6.828 ± 0.377

5.66 ± 0.443

6.428 ± 0.386

1.343 ± 0.147

3.134 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski