Salmonella phage Vi II-E1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1GS69|B1GS69_9CAUD Hypothetical phage protein OS=Salmonella phage Vi II-E1 OX=424716 GN=VIP0013 PE=4 SV=1
MM1 pKa = 7.53NDD3 pKa = 3.45EE4 pKa = 5.13GLLEE8 pKa = 4.44CPFCGSLEE16 pKa = 4.13VYY18 pKa = 10.71LFDD21 pKa = 4.66EE22 pKa = 5.08LVLSHH27 pKa = 6.61VSCSSCDD34 pKa = 3.38ARR36 pKa = 11.84TDD38 pKa = 3.52DD39 pKa = 5.39HH40 pKa = 7.29FDD42 pKa = 2.82ASMAVKK48 pKa = 10.01SWNTRR53 pKa = 11.84NGHH56 pKa = 5.98VYY58 pKa = 9.12TADD61 pKa = 3.62DD62 pKa = 4.4FSRR65 pKa = 11.84AAEE68 pKa = 3.83EE69 pKa = 4.24RR70 pKa = 11.84EE71 pKa = 4.04YY72 pKa = 11.66GLL74 pKa = 4.69

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1GS35|B1GS35_9CAUD Putative exodeoxyribonuclease OS=Salmonella phage Vi II-E1 OX=424716 GN=VIP0043 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 5.97IKK4 pKa = 9.99PCKK7 pKa = 10.14NMEE10 pKa = 4.17KK11 pKa = 10.15SSPAGTMRR19 pKa = 11.84NDD21 pKa = 3.31GYY23 pKa = 11.01LQVRR27 pKa = 11.84FMNEE31 pKa = 2.36IHH33 pKa = 6.93LIHH36 pKa = 6.9RR37 pKa = 11.84IVWEE41 pKa = 4.1MFNGEE46 pKa = 4.84IPDD49 pKa = 3.81GMQIDD54 pKa = 5.24HH55 pKa = 7.06INHH58 pKa = 6.64DD59 pKa = 3.86RR60 pKa = 11.84GDD62 pKa = 3.54NRR64 pKa = 11.84IQNLRR69 pKa = 11.84MVTKK73 pKa = 10.53ISNGRR78 pKa = 11.84NLTKK82 pKa = 10.51KK83 pKa = 10.14INNTSGVTGVSWYY96 pKa = 10.32KK97 pKa = 10.89SRR99 pKa = 11.84GKK101 pKa = 9.5WRR103 pKa = 11.84VQIMVDD109 pKa = 3.28RR110 pKa = 11.84KK111 pKa = 10.1SIHH114 pKa = 6.21IGYY117 pKa = 10.23FSDD120 pKa = 4.04FEE122 pKa = 4.53SAVAARR128 pKa = 11.84ISANEE133 pKa = 3.61RR134 pKa = 11.84YY135 pKa = 9.8GFHH138 pKa = 7.18KK139 pKa = 10.79NHH141 pKa = 6.47GVNCC145 pKa = 4.25

Molecular weight: 16.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12414

59

980

243.4

27.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.523 ± 0.652

1.434 ± 0.223

6.082 ± 0.325

6.493 ± 0.293

3.464 ± 0.2

7.065 ± 0.258

1.966 ± 0.245

6.267 ± 0.194

6.477 ± 0.412

7.185 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.134 ± 0.203

4.898 ± 0.24

3.287 ± 0.207

4.253 ± 0.371

5.292 ± 0.259

6.742 ± 0.345

5.703 ± 0.435

6.509 ± 0.24

1.482 ± 0.136

3.746 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski