Beet western yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8JZ11|Q8JZ11_9LUTE Serine protease OS=Beet western yellows virus OX=12042 PE=4 SV=1
SS1 pKa = 7.6GDD3 pKa = 3.45RR4 pKa = 11.84QEE6 pKa = 4.12LGKK9 pKa = 10.38RR10 pKa = 11.84EE11 pKa = 4.15SGSARR16 pKa = 11.84RR17 pKa = 11.84NNRR20 pKa = 11.84NPQRR24 pKa = 11.84KK25 pKa = 8.26HH26 pKa = 4.39PQGRR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 7.95QWRR35 pKa = 11.84RR36 pKa = 11.84NPPSCCSYY44 pKa = 9.59FTEE47 pKa = 4.74GTLGPRR53 pKa = 11.84CHH55 pKa = 6.92ASHH58 pKa = 6.26VHH60 pKa = 5.52TPDD63 pKa = 5.51DD64 pKa = 4.38EE65 pKa = 4.55GQPGCWCEE73 pKa = 4.4VPNHH77 pKa = 6.67DD78 pKa = 4.18CFFRR82 pKa = 11.84RR83 pKa = 11.84FQRR86 pKa = 11.84DD87 pKa = 3.52QRR89 pKa = 11.84SHH91 pKa = 6.42PAEE94 pKa = 3.99DD95 pKa = 4.05RR96 pKa = 11.84YY97 pKa = 11.55SFDD100 pKa = 3.64RR101 pKa = 11.84EE102 pKa = 4.0AGRR105 pKa = 11.84GDD107 pKa = 3.38PCRR110 pKa = 11.84EE111 pKa = 4.18GNEE114 pKa = 3.96EE115 pKa = 4.17TPEE118 pKa = 3.84SRR120 pKa = 11.84AEE122 pKa = 4.24KK123 pKa = 9.93ICEE126 pKa = 3.73QAEE129 pKa = 4.21NFCQYY134 pKa = 10.52FSSLYY139 pKa = 9.16RR140 pKa = 11.84WEE142 pKa = 4.41SGSGKK147 pKa = 9.24EE148 pKa = 4.47APGFEE153 pKa = 4.62QIGSLPQFYY162 pKa = 10.3HH163 pKa = 6.83PKK165 pKa = 10.02QKK167 pKa = 11.06GEE169 pKa = 4.25SRR171 pKa = 11.84WGAKK175 pKa = 8.9VLQLHH180 pKa = 7.05PEE182 pKa = 4.34LEE184 pKa = 4.15AQTRR188 pKa = 11.84GFGWPQFGAQAEE200 pKa = 4.48LKK202 pKa = 10.45SLRR205 pKa = 11.84LQAARR210 pKa = 11.84WLEE213 pKa = 3.69RR214 pKa = 11.84AQQVKK219 pKa = 10.17IPSTEE224 pKa = 3.39EE225 pKa = 3.52RR226 pKa = 11.84EE227 pKa = 3.96RR228 pKa = 11.84VIRR231 pKa = 11.84KK232 pKa = 9.1CCEE235 pKa = 3.37AYY237 pKa = 10.63QNAKK241 pKa = 10.68SNGPNATRR249 pKa = 11.84GNKK252 pKa = 9.79LSWDD256 pKa = 3.5NFLEE260 pKa = 4.26DD261 pKa = 4.1FKK263 pKa = 11.41QAVFSLEE270 pKa = 3.42FDD272 pKa = 3.34AGIGVPYY279 pKa = 9.92IAYY282 pKa = 8.45GKK284 pKa = 6.46PTHH287 pKa = 6.61RR288 pKa = 11.84GWVEE292 pKa = 3.85DD293 pKa = 3.95QEE295 pKa = 4.56LLPILARR302 pKa = 11.84LTFIRR307 pKa = 11.84LQKK310 pKa = 9.52MLEE313 pKa = 3.84ARR315 pKa = 11.84FEE317 pKa = 4.18EE318 pKa = 4.71LSPEE322 pKa = 4.01QLVQEE327 pKa = 4.7GLCDD331 pKa = 4.87PIRR334 pKa = 11.84VFVKK338 pKa = 10.78GEE340 pKa = 3.59PHH342 pKa = 6.19KK343 pKa = 10.54QSKK346 pKa = 10.21LDD348 pKa = 3.38EE349 pKa = 4.27GRR351 pKa = 11.84YY352 pKa = 8.96RR353 pKa = 11.84LIMSVSLVDD362 pKa = 3.45QLVARR367 pKa = 11.84VLFQNQNKK375 pKa = 9.66RR376 pKa = 11.84EE377 pKa = 3.95IALWRR382 pKa = 11.84AVPSKK387 pKa = 10.57PGFGLSTDD395 pKa = 3.6EE396 pKa = 3.99QVVEE400 pKa = 4.75FVQMLSAQVKK410 pKa = 8.85VRR412 pKa = 11.84PPQLISDD419 pKa = 4.26WRR421 pKa = 11.84QHH423 pKa = 6.52LVATDD428 pKa = 3.61CSGFDD433 pKa = 3.32WSVSDD438 pKa = 4.58WLLEE442 pKa = 4.07DD443 pKa = 4.28DD444 pKa = 3.82MEE446 pKa = 4.4VRR448 pKa = 11.84NRR450 pKa = 11.84LTTDD454 pKa = 3.34LNEE457 pKa = 4.31TTSKK461 pKa = 10.7LRR463 pKa = 11.84AAWLKK468 pKa = 10.69CISNSVLCLSDD479 pKa = 3.31GTLLAQRR486 pKa = 11.84VPGVQKK492 pKa = 10.45SGSYY496 pKa = 8.25NTSSSNSRR504 pKa = 11.84IRR506 pKa = 11.84VMAAFHH512 pKa = 6.86CGAEE516 pKa = 3.94WAMAMGDD523 pKa = 3.77DD524 pKa = 4.0ALEE527 pKa = 4.35SVSTDD532 pKa = 3.08LGKK535 pKa = 10.78YY536 pKa = 8.84AALGFKK542 pKa = 10.44VEE544 pKa = 4.31EE545 pKa = 4.12SSKK548 pKa = 11.47LEE550 pKa = 3.98FCSHH554 pKa = 5.14IFEE557 pKa = 5.18RR558 pKa = 11.84EE559 pKa = 3.87DD560 pKa = 3.57LAVPVNKK567 pKa = 10.6AKK569 pKa = 10.09MIYY572 pKa = 10.08KK573 pKa = 9.93LIHH576 pKa = 6.3GYY578 pKa = 9.39EE579 pKa = 4.21PEE581 pKa = 4.62CGNPEE586 pKa = 4.01VLVNYY591 pKa = 7.63LTACFAILNEE601 pKa = 4.31LRR603 pKa = 11.84SDD605 pKa = 3.53PQMVQTLYY613 pKa = 10.34SWLVKK618 pKa = 9.87PVQPQKK624 pKa = 11.28NN625 pKa = 3.49

Molecular weight: 71.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8JZ09|Q8JZ09_9LUTE Readthrough protein OS=Beet western yellows virus OX=12042 PE=3 SV=1
MM1 pKa = 6.49NTVVGRR7 pKa = 11.84RR8 pKa = 11.84TINGRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84QARR21 pKa = 11.84RR22 pKa = 11.84AQRR25 pKa = 11.84SQPVVVVQTSRR36 pKa = 11.84TTQRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GNNRR51 pKa = 11.84TRR53 pKa = 11.84GAVSTRR59 pKa = 11.84GASSSEE65 pKa = 3.81TFVFSKK71 pKa = 11.13DD72 pKa = 3.3NLAGSSSGAITFGPSLSDD90 pKa = 4.1CPAFSNGILKK100 pKa = 10.26AYY102 pKa = 10.04HH103 pKa = 6.58EE104 pKa = 4.78YY105 pKa = 10.01KK106 pKa = 10.07ISMVILEE113 pKa = 4.63FVSEE117 pKa = 4.2ASSQNSGSIAYY128 pKa = 9.28EE129 pKa = 3.87LDD131 pKa = 3.1PHH133 pKa = 6.75CRR135 pKa = 11.84LDD137 pKa = 4.04ALSSTINKK145 pKa = 9.79FGITKK150 pKa = 9.13PGRR153 pKa = 11.84RR154 pKa = 11.84AFTASYY160 pKa = 10.99INGTDD165 pKa = 2.79WHH167 pKa = 7.19DD168 pKa = 3.51VAKK171 pKa = 10.53DD172 pKa = 3.32QFRR175 pKa = 11.84ILYY178 pKa = 8.84KK179 pKa = 11.0GNGSSSIAGSFRR191 pKa = 11.84ITMKK195 pKa = 10.75CQFHH199 pKa = 6.24NPKK202 pKa = 10.51

Molecular weight: 22.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2545

175

670

424.2

47.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.269 ± 0.526

1.768 ± 0.412

4.086 ± 0.788

6.09 ± 0.762

3.929 ± 0.37

6.798 ± 0.242

2.24 ± 0.329

4.126 ± 0.479

5.383 ± 0.454

7.583 ± 1.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.234

4.283 ± 0.437

5.815 ± 0.514

4.715 ± 0.575

7.623 ± 0.887

9.98 ± 0.933

6.051 ± 1.051

5.855 ± 0.334

1.768 ± 0.234

2.633 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski