Amino acid dipepetide frequency for Aspergillus mulundensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.213AlaAla: 9.213 ± 0.051	1.113AlaCys: 1.113 ± 0.016	4.496AlaAsp: 4.496 ± 0.031	5.385AlaGlu: 5.385 ± 0.038	3.188AlaPhe: 3.188 ± 0.024	6.09AlaGly: 6.09 ± 0.044	1.885AlaHis: 1.885 ± 0.021	4.335AlaIle: 4.335 ± 0.032	3.746AlaLys: 3.746 ± 0.033	8.283AlaLeu: 8.283 ± 0.042	1.979AlaMet: 1.979 ± 0.019	2.922AlaAsn: 2.922 ± 0.025	4.831AlaPro: 4.831 ± 0.041	3.492AlaGln: 3.492 ± 0.036	5.214AlaArg: 5.214 ± 0.036	7.297AlaSer: 7.297 ± 0.045	5.247AlaThr: 5.247 ± 0.035	5.725AlaVal: 5.725 ± 0.032	1.225AlaTrp: 1.225 ± 0.016	2.24AlaTyr: 2.24 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	1.022CysAla: 1.022 ± 0.016	0.242CysCys: 0.242 ± 0.008	0.692CysAsp: 0.692 ± 0.013	0.626CysGlu: 0.626 ± 0.011	0.518CysPhe: 0.518 ± 0.009	0.928CysGly: 0.928 ± 0.014	0.323CysHis: 0.323 ± 0.008	0.698CysIle: 0.698 ± 0.011	0.457CysLys: 0.457 ± 0.01	1.312CysLeu: 1.312 ± 0.017	0.276CysMet: 0.276 ± 0.006	0.414CysAsn: 0.414 ± 0.008	0.685CysPro: 0.685 ± 0.012	0.434CysGln: 0.434 ± 0.009	0.803CysArg: 0.803 ± 0.013	0.934CysSer: 0.934 ± 0.015	0.719CysThr: 0.719 ± 0.012	0.844CysVal: 0.844 ± 0.013	0.208CysTrp: 0.208 ± 0.007	0.352CysTyr: 0.352 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.949AspAla: 4.949 ± 0.029	0.661AspCys: 0.661 ± 0.013	4.095AspAsp: 4.095 ± 0.04	4.529AspGlu: 4.529 ± 0.041	2.192AspPhe: 2.192 ± 0.02	4.134AspGly: 4.134 ± 0.031	1.26AspHis: 1.26 ± 0.014	3.145AspIle: 3.145 ± 0.025	2.251AspLys: 2.251 ± 0.019	5.269AspLeu: 5.269 ± 0.033	1.219AspMet: 1.219 ± 0.015	1.859AspAsn: 1.859 ± 0.017	3.414AspPro: 3.414 ± 0.027	1.849AspGln: 1.849 ± 0.018	3.123AspArg: 3.123 ± 0.029	4.176AspSer: 4.176 ± 0.036	3.037AspThr: 3.037 ± 0.027	3.707AspVal: 3.707 ± 0.027	0.915AspTrp: 0.915 ± 0.015	1.642AspTyr: 1.642 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.393GluAla: 5.393 ± 0.036	0.671GluCys: 0.671 ± 0.012	4.144GluAsp: 4.144 ± 0.034	5.289GluGlu: 5.289 ± 0.048	1.989GluPhe: 1.989 ± 0.018	3.894GluGly: 3.894 ± 0.033	1.464GluHis: 1.464 ± 0.018	3.108GluIle: 3.108 ± 0.024	3.412GluLys: 3.412 ± 0.032	5.276GluLeu: 5.276 ± 0.035	1.433GluMet: 1.433 ± 0.015	2.305GluAsn: 2.305 ± 0.021	2.947GluPro: 2.947 ± 0.032	2.484GluGln: 2.484 ± 0.024	4.055GluArg: 4.055 ± 0.033	4.386GluSer: 4.386 ± 0.031	3.691GluThr: 3.691 ± 0.029	3.568GluVal: 3.568 ± 0.027	0.895GluTrp: 0.895 ± 0.013	1.795GluTyr: 1.795 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.096PheAla: 3.096 ± 0.027	0.542PheCys: 0.542 ± 0.009	2.304PheAsp: 2.304 ± 0.022	2.101PheGlu: 2.101 ± 0.02	1.585PhePhe: 1.585 ± 0.021	2.854PheGly: 2.854 ± 0.025	0.934PheHis: 0.934 ± 0.013	1.757PheIle: 1.757 ± 0.021	1.374PheLys: 1.374 ± 0.018	3.523PheLeu: 3.523 ± 0.032	0.759PheMet: 0.759 ± 0.011	1.386PheAsn: 1.386 ± 0.017	1.996PhePro: 1.996 ± 0.019	1.334PheGln: 1.334 ± 0.015	2.006PheArg: 2.006 ± 0.021	2.94PheSer: 2.94 ± 0.026	2.156PheThr: 2.156 ± 0.021	2.366PheVal: 2.366 ± 0.023	0.638PheTrp: 0.638 ± 0.011	1.12PheTyr: 1.12 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	5.534GlyAla: 5.534 ± 0.037	0.93GlyCys: 0.93 ± 0.015	3.733GlyAsp: 3.733 ± 0.03	3.783GlyGlu: 3.783 ± 0.03	2.81GlyPhe: 2.81 ± 0.026	5.592GlyGly: 5.592 ± 0.07	1.755GlyHis: 1.755 ± 0.019	3.52GlyIle: 3.52 ± 0.027	3.223GlyLys: 3.223 ± 0.027	6.373GlyLeu: 6.373 ± 0.035	1.528GlyMet: 1.528 ± 0.017	2.519GlyAsn: 2.519 ± 0.024	3.455GlyPro: 3.455 ± 0.032	2.529GlyGln: 2.529 ± 0.025	4.173GlyArg: 4.173 ± 0.03	5.7GlySer: 5.7 ± 0.057	4.013GlyThr: 4.013 ± 0.03	4.625GlyVal: 4.625 ± 0.031	1.199GlyTrp: 1.199 ± 0.018	2.182GlyTyr: 2.182 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	2.005HisAla: 2.005 ± 0.019	0.334HisCys: 0.334 ± 0.009	1.309HisAsp: 1.309 ± 0.015	1.369HisGlu: 1.369 ± 0.017	0.92HisPhe: 0.92 ± 0.012	1.839HisGly: 1.839 ± 0.019	0.82HisHis: 0.82 ± 0.015	1.244HisIle: 1.244 ± 0.014	0.846HisLys: 0.846 ± 0.013	2.329HisLeu: 2.329 ± 0.022	0.468HisMet: 0.468 ± 0.009	0.835HisAsn: 0.835 ± 0.011	1.728HisPro: 1.728 ± 0.018	0.928HisGln: 0.928 ± 0.012	1.62HisArg: 1.62 ± 0.021	1.842HisSer: 1.842 ± 0.02	1.29HisThr: 1.29 ± 0.015	1.426HisVal: 1.426 ± 0.016	0.379HisTrp: 0.379 ± 0.009	0.725HisTyr: 0.725 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.326IleAla: 4.326 ± 0.029	0.742IleCys: 0.742 ± 0.013	2.822IleAsp: 2.822 ± 0.021	2.838IleGlu: 2.838 ± 0.023	1.912IlePhe: 1.912 ± 0.021	3.144IleGly: 3.144 ± 0.027	1.216IleHis: 1.216 ± 0.016	2.416IleIle: 2.416 ± 0.027	1.977IleLys: 1.977 ± 0.023	4.622IleLeu: 4.622 ± 0.031	0.947IleMet: 0.947 ± 0.013	1.741IleAsn: 1.741 ± 0.018	3.186IlePro: 3.186 ± 0.027	1.918IleGln: 1.918 ± 0.019	2.855IleArg: 2.855 ± 0.023	3.75IleSer: 3.75 ± 0.027	2.852IleThr: 2.852 ± 0.025	3.124IleVal: 3.124 ± 0.028	0.728IleTrp: 0.728 ± 0.013	1.433IleTyr: 1.433 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.993LysAla: 3.993 ± 0.032	0.468LysCys: 0.468 ± 0.01	2.56LysAsp: 2.56 ± 0.023	3.146LysGlu: 3.146 ± 0.03	1.288LysPhe: 1.288 ± 0.016	2.789LysGly: 2.789 ± 0.024	1.053LysHis: 1.053 ± 0.014	2.051LysIle: 2.051 ± 0.018	2.788LysLys: 2.788 ± 0.036	3.83LysLeu: 3.83 ± 0.027	0.859LysMet: 0.859 ± 0.011	1.535LysAsn: 1.535 ± 0.017	2.587LysPro: 2.587 ± 0.027	1.719LysGln: 1.719 ± 0.019	3.212LysArg: 3.212 ± 0.029	3.137LysSer: 3.137 ± 0.029	2.576LysThr: 2.576 ± 0.025	2.588LysVal: 2.588 ± 0.021	0.625LysTrp: 0.625 ± 0.009	1.305LysTyr: 1.305 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	8.212LeuAla: 8.212 ± 0.046	1.235LeuCys: 1.235 ± 0.017	5.478LeuAsp: 5.478 ± 0.033	5.676LeuGlu: 5.676 ± 0.037	3.367LeuPhe: 3.367 ± 0.032	6.157LeuGly: 6.157 ± 0.039	2.384LeuHis: 2.384 ± 0.024	3.963LeuIle: 3.963 ± 0.03	3.967LeuLys: 3.967 ± 0.03	8.799LeuLeu: 8.799 ± 0.064	1.76LeuMet: 1.76 ± 0.02	3.248LeuAsn: 3.248 ± 0.022	5.617LeuPro: 5.617 ± 0.037	3.86LeuGln: 3.86 ± 0.028	6.006LeuArg: 6.006 ± 0.036	7.516LeuSer: 7.516 ± 0.044	4.985LeuThr: 4.985 ± 0.028	5.593LeuVal: 5.593 ± 0.036	1.247LeuTrp: 1.247 ± 0.017	2.512LeuTyr: 2.512 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.143MetAla: 2.143 ± 0.019	0.236MetCys: 0.236 ± 0.006	1.216MetAsp: 1.216 ± 0.016	1.231MetGlu: 1.231 ± 0.017	0.695MetPhe: 0.695 ± 0.013	1.411MetGly: 1.411 ± 0.016	0.487MetHis: 0.487 ± 0.01	0.941MetIle: 0.941 ± 0.014	0.895MetLys: 0.895 ± 0.013	1.832MetLeu: 1.832 ± 0.018	0.497MetMet: 0.497 ± 0.011	0.755MetAsn: 0.755 ± 0.012	1.224MetPro: 1.224 ± 0.012	0.811MetGln: 0.811 ± 0.013	1.238MetArg: 1.238 ± 0.014	1.745MetSer: 1.745 ± 0.018	1.191MetThr: 1.191 ± 0.015	1.301MetVal: 1.301 ± 0.016	0.248MetTrp: 0.248 ± 0.007	0.503MetTyr: 0.503 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.189AsnAla: 3.189 ± 0.026	0.452AsnCys: 0.452 ± 0.011	1.921AsnAsp: 1.921 ± 0.017	2.034AsnGlu: 2.034 ± 0.021	1.242AsnPhe: 1.242 ± 0.016	2.905AsnGly: 2.905 ± 0.026	0.841AsnHis: 0.841 ± 0.013	1.979AsnIle: 1.979 ± 0.02	1.42AsnLys: 1.42 ± 0.016	3.198AsnLeu: 3.198 ± 0.024	0.773AsnMet: 0.773 ± 0.012	1.393AsnAsn: 1.393 ± 0.017	2.52AsnPro: 2.52 ± 0.023	1.314AsnGln: 1.314 ± 0.016	1.983AsnArg: 1.983 ± 0.021	2.61AsnSer: 2.61 ± 0.024	2.191AsnThr: 2.191 ± 0.02	2.271AsnVal: 2.271 ± 0.02	0.564AsnTrp: 0.564 ± 0.008	1.038AsnTyr: 1.038 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	5.423ProAla: 5.423 ± 0.045	0.567ProCys: 0.567 ± 0.012	3.437ProAsp: 3.437 ± 0.024	3.982ProGlu: 3.982 ± 0.032	2.12ProPhe: 2.12 ± 0.022	4.139ProGly: 4.139 ± 0.038	1.346ProHis: 1.346 ± 0.017	2.545ProIle: 2.545 ± 0.023	2.437ProLys: 2.437 ± 0.025	4.905ProLeu: 4.905 ± 0.033	1.011ProMet: 1.011 ± 0.014	2.205ProAsn: 2.205 ± 0.022	4.808ProPro: 4.808 ± 0.063	2.44ProGln: 2.44 ± 0.028	3.572ProArg: 3.572 ± 0.031	6.134ProSer: 6.134 ± 0.048	3.999ProThr: 3.999 ± 0.036	3.731ProVal: 3.731 ± 0.033	0.78ProTrp: 0.78 ± 0.011	1.544ProTyr: 1.544 ± 0.015	0.0ProXaa: 0.0 ± 0.0
Gln	3.398GlnAla: 3.398 ± 0.032	0.455GlnCys: 0.455 ± 0.01	2.055GlnAsp: 2.055 ± 0.018	2.359GlnGlu: 2.359 ± 0.024	1.266GlnPhe: 1.266 ± 0.013	2.413GlnGly: 2.413 ± 0.021	1.033GlnHis: 1.033 ± 0.013	1.816GlnIle: 1.816 ± 0.018	1.795GlnLys: 1.795 ± 0.018	3.513GlnLeu: 3.513 ± 0.03	0.838GlnMet: 0.838 ± 0.012	1.491GlnAsn: 1.491 ± 0.016	2.584GlnPro: 2.584 ± 0.031	2.14GlnGln: 2.14 ± 0.034	2.661GlnArg: 2.661 ± 0.028	3.106GlnSer: 3.106 ± 0.028	2.377GlnThr: 2.377 ± 0.022	2.16GlnVal: 2.16 ± 0.019	0.571GlnTrp: 0.571 ± 0.01	1.144GlnTyr: 1.144 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	4.98ArgAla: 4.98 ± 0.03	0.746ArgCys: 0.746 ± 0.012	3.483ArgAsp: 3.483 ± 0.031	3.902ArgGlu: 3.902 ± 0.033	2.242ArgPhe: 2.242 ± 0.02	3.884ArgGly: 3.884 ± 0.032	1.606ArgHis: 1.606 ± 0.018	2.942ArgIle: 2.942 ± 0.022	3.259ArgLys: 3.259 ± 0.028	5.811ArgLeu: 5.811 ± 0.034	1.302ArgMet: 1.302 ± 0.014	2.274ArgAsn: 2.274 ± 0.022	3.619ArgPro: 3.619 ± 0.03	2.535ArgGln: 2.535 ± 0.024	5.166ArgArg: 5.166 ± 0.046	4.749ArgSer: 4.749 ± 0.038	3.412ArgThr: 3.412 ± 0.025	3.612ArgVal: 3.612 ± 0.025	0.987ArgTrp: 0.987 ± 0.014	1.74ArgTyr: 1.74 ± 0.017	0.0ArgXaa: 0.0 ± 0.0
Ser	6.845SerAla: 6.845 ± 0.048	0.882SerCys: 0.882 ± 0.016	4.337SerAsp: 4.337 ± 0.031	4.276SerGlu: 4.276 ± 0.031	3.005SerPhe: 3.005 ± 0.026	5.567SerGly: 5.567 ± 0.042	1.931SerHis: 1.931 ± 0.021	3.926SerIle: 3.926 ± 0.025	3.371SerLys: 3.371 ± 0.028	7.359SerLeu: 7.359 ± 0.04	1.578SerMet: 1.578 ± 0.017	2.877SerAsn: 2.877 ± 0.022	5.557SerPro: 5.557 ± 0.045	3.232SerGln: 3.232 ± 0.029	5.008SerArg: 5.008 ± 0.04	8.51SerSer: 8.51 ± 0.072	5.698SerThr: 5.698 ± 0.05	4.569SerVal: 4.569 ± 0.032	1.144SerTrp: 1.144 ± 0.015	2.043SerTyr: 2.043 ± 0.019	0.0SerXaa: 0.0 ± 0.0
Thr	5.395ThrAla: 5.395 ± 0.031	0.753ThrCys: 0.753 ± 0.013	2.964ThrAsp: 2.964 ± 0.023	3.257ThrGlu: 3.257 ± 0.025	2.221ThrPhe: 2.221 ± 0.017	4.306ThrGly: 4.306 ± 0.032	1.322ThrHis: 1.322 ± 0.016	3.021ThrIle: 3.021 ± 0.024	2.377ThrLys: 2.377 ± 0.024	5.439ThrLeu: 5.439 ± 0.034	1.134ThrMet: 1.134 ± 0.015	2.065ThrAsn: 2.065 ± 0.02	4.453ThrPro: 4.453 ± 0.035	2.11ThrGln: 2.11 ± 0.022	3.156ThrArg: 3.156 ± 0.025	5.27ThrSer: 5.27 ± 0.048	4.189ThrThr: 4.189 ± 0.04	3.848ThrVal: 3.848 ± 0.03	0.894ThrTrp: 0.894 ± 0.014	1.619ThrTyr: 1.619 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	5.253ValAla: 5.253 ± 0.034	0.852ValCys: 0.852 ± 0.011	3.802ValAsp: 3.802 ± 0.027	3.945ValGlu: 3.945 ± 0.029	2.48ValPhe: 2.48 ± 0.025	4.074ValGly: 4.074 ± 0.028	1.446ValHis: 1.446 ± 0.018	2.942ValIle: 2.942 ± 0.026	2.688ValLys: 2.688 ± 0.023	5.827ValLeu: 5.827 ± 0.039	1.257ValMet: 1.257 ± 0.015	2.247ValAsn: 2.247 ± 0.021	3.675ValPro: 3.675 ± 0.03	2.432ValGln: 2.432 ± 0.02	3.667ValArg: 3.667 ± 0.026	4.812ValSer: 4.812 ± 0.032	3.5ValThr: 3.5 ± 0.024	4.358ValVal: 4.358 ± 0.034	0.889ValTrp: 0.889 ± 0.015	1.837ValTyr: 1.837 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.219TrpAla: 1.219 ± 0.015	0.196TrpCys: 0.196 ± 0.006	0.924TrpAsp: 0.924 ± 0.013	0.902TrpGlu: 0.902 ± 0.012	0.542TrpPhe: 0.542 ± 0.009	0.947TrpGly: 0.947 ± 0.013	0.365TrpHis: 0.365 ± 0.008	0.763TrpIle: 0.763 ± 0.013	0.773TrpLys: 0.773 ± 0.013	1.37TrpLeu: 1.37 ± 0.017	0.369TrpMet: 0.369 ± 0.008	0.647TrpAsn: 0.647 ± 0.011	0.635TrpPro: 0.635 ± 0.012	0.536TrpGln: 0.536 ± 0.01	1.006TrpArg: 1.006 ± 0.012	1.069TrpSer: 1.069 ± 0.014	0.955TrpThr: 0.955 ± 0.014	0.931TrpVal: 0.931 ± 0.013	0.281TrpTrp: 0.281 ± 0.007	0.442TrpTyr: 0.442 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.321TyrAla: 2.321 ± 0.021	0.414TyrCys: 0.414 ± 0.01	1.657TyrAsp: 1.657 ± 0.019	1.57TyrGlu: 1.57 ± 0.017	1.189TyrPhe: 1.189 ± 0.016	2.109TyrGly: 2.109 ± 0.022	0.786TyrHis: 0.786 ± 0.011	1.437TyrIle: 1.437 ± 0.017	1.05TyrLys: 1.05 ± 0.014	2.71TyrLeu: 2.71 ± 0.024	0.611TyrMet: 0.611 ± 0.01	1.119TyrAsn: 1.119 ± 0.013	1.602TyrPro: 1.602 ± 0.016	1.076TyrGln: 1.076 ± 0.015	1.726TyrArg: 1.726 ± 0.019	2.03TyrSer: 2.03 ± 0.02	1.716TyrThr: 1.716 ± 0.017	1.639TyrVal: 1.639 ± 0.016	0.454TyrTrp: 0.454 ± 0.009	0.961TyrTyr: 0.961 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11569 proteins (5851458 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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