Beihai sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KED6|A0A1L3KED6_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 1 OX=1922680 PE=4 SV=1
MM1 pKa = 7.68PSKK4 pKa = 9.8KK5 pKa = 8.65TRR7 pKa = 11.84EE8 pKa = 3.65IHH10 pKa = 5.57KK11 pKa = 9.9GEE13 pKa = 3.53RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84ARR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 9.42RR24 pKa = 11.84NKK26 pKa = 9.63QLAHH30 pKa = 5.59GQKK33 pKa = 10.41GPQRR37 pKa = 11.84AQEE40 pKa = 4.16STPLRR45 pKa = 11.84DD46 pKa = 2.98VGSALGGIAGSLLGAPKK63 pKa = 10.05IGSLLGGALGSGVGKK78 pKa = 10.56LFGSGEE84 pKa = 3.92YY85 pKa = 10.36HH86 pKa = 6.71EE87 pKa = 5.5ALEE90 pKa = 4.59SEE92 pKa = 4.52LGAPVAEE99 pKa = 4.47PADD102 pKa = 3.75MPEE105 pKa = 4.32TNSLVEE111 pKa = 4.23PLSSNDD117 pKa = 3.91LVPLMHH123 pKa = 6.81QDD125 pKa = 3.11QEE127 pKa = 4.63GAVTITRR134 pKa = 11.84RR135 pKa = 11.84EE136 pKa = 4.16YY137 pKa = 10.54IRR139 pKa = 11.84DD140 pKa = 3.29ITILNSGSNFNFYY153 pKa = 10.14IGPGQQQFVWLSGIAKK169 pKa = 9.49SWQKK173 pKa = 10.42FAFTGLAMEE182 pKa = 4.31YY183 pKa = 10.57VPLSGTAVSSTSAALGQVAMCFSYY207 pKa = 11.15NVVEE211 pKa = 5.33STAAWPRR218 pKa = 11.84GVNAAILNMNGAVGCSPAAAATCYY242 pKa = 9.77MEE244 pKa = 5.19CDD246 pKa = 3.27PRR248 pKa = 11.84QAVNPFCFINNEE260 pKa = 3.95QPAVANWSEE269 pKa = 4.0SDD271 pKa = 3.68YY272 pKa = 11.24FPAEE276 pKa = 3.69FLLRR280 pKa = 11.84TSGAQEE286 pKa = 3.72LTTPFVCGALWCTYY300 pKa = 9.57EE301 pKa = 4.33VVLLEE306 pKa = 4.62PRR308 pKa = 11.84PVDD311 pKa = 3.57PSPALSFMNMPAFKK325 pKa = 10.84DD326 pKa = 4.0FVSVLNEE333 pKa = 3.47KK334 pKa = 10.31RR335 pKa = 11.84VLSNHH340 pKa = 6.43CGPYY344 pKa = 9.02TDD346 pKa = 3.57KK347 pKa = 11.23QVMLRR352 pKa = 11.84AAEE355 pKa = 4.06MRR357 pKa = 11.84RR358 pKa = 11.84LAAILRR364 pKa = 11.84SLPFADD370 pKa = 3.83ALEE373 pKa = 4.34KK374 pKa = 10.86ARR376 pKa = 11.84LQSWSIRR383 pKa = 11.84EE384 pKa = 4.09EE385 pKa = 4.35DD386 pKa = 4.14DD387 pKa = 3.78TATDD391 pKa = 3.31QQDD394 pKa = 3.54QVDD397 pKa = 5.4LIVSQLEE404 pKa = 4.09AKK406 pKa = 10.25YY407 pKa = 10.15KK408 pKa = 10.55ALSTEE413 pKa = 3.66MDD415 pKa = 3.2TWVQPVLTSTGSKK428 pKa = 8.98RR429 pKa = 11.84PKK431 pKa = 9.61YY432 pKa = 10.41

Molecular weight: 47.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KED8|A0A1L3KED8_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 1 OX=1922680 PE=4 SV=1
MM1 pKa = 7.59GPMEE5 pKa = 4.2MAVPRR10 pKa = 11.84LEE12 pKa = 4.67GDD14 pKa = 3.7TILIDD19 pKa = 3.79AGGEE23 pKa = 3.76IYY25 pKa = 10.29EE26 pKa = 4.44FSMTVTEE33 pKa = 5.67FSDD36 pKa = 4.45LVLKK40 pKa = 10.63QRR42 pKa = 11.84GRR44 pKa = 11.84MLFCEE49 pKa = 4.68RR50 pKa = 11.84AQEE53 pKa = 3.89LAKK56 pKa = 10.34PVIFDD61 pKa = 4.08PSPQYY66 pKa = 10.73RR67 pKa = 11.84RR68 pKa = 11.84VDD70 pKa = 3.7VKK72 pKa = 11.02EE73 pKa = 3.95STLPGSKK80 pKa = 9.92HH81 pKa = 6.63RR82 pKa = 11.84ILHH85 pKa = 5.72SLPNSCVQIHH95 pKa = 6.76AEE97 pKa = 4.08PGAHH101 pKa = 7.06RR102 pKa = 11.84LTGVHH107 pKa = 6.15GGGVRR112 pKa = 11.84VVYY115 pKa = 10.09GKK117 pKa = 8.44KK118 pKa = 8.39TYY120 pKa = 10.51LYY122 pKa = 8.35TAYY125 pKa = 10.31HH126 pKa = 5.98VIEE129 pKa = 5.0HH130 pKa = 7.0LLSQDD135 pKa = 3.51GVDD138 pKa = 4.5TICLRR143 pKa = 11.84ANGNDD148 pKa = 3.1VQMEE152 pKa = 3.93KK153 pKa = 10.9DD154 pKa = 3.35LRR156 pKa = 11.84FVAFSRR162 pKa = 11.84ALDD165 pKa = 3.64FVVIDD170 pKa = 3.64VPEE173 pKa = 4.24RR174 pKa = 11.84AWSKK178 pKa = 10.14LQVKK182 pKa = 9.13AAKK185 pKa = 9.46LAKK188 pKa = 9.42NWPSGAAVRR197 pKa = 11.84AIHH200 pKa = 7.1CDD202 pKa = 3.47GAGKK206 pKa = 10.61ASMAHH211 pKa = 7.06GYY213 pKa = 8.29LQSMEE218 pKa = 4.61KK219 pKa = 10.82GLLTHH224 pKa = 6.78TASTRR229 pKa = 11.84KK230 pKa = 9.78GSSGFPLMNLGGNLVYY246 pKa = 10.31GVHH249 pKa = 5.95VSGASTLNKK258 pKa = 9.71CSPAFFPFGGRR269 pKa = 11.84RR270 pKa = 11.84GASKK274 pKa = 10.66KK275 pKa = 10.54SADD278 pKa = 3.42GVLEE282 pKa = 3.99SSRR285 pKa = 11.84DD286 pKa = 3.75DD287 pKa = 3.9YY288 pKa = 11.77PDD290 pKa = 4.83DD291 pKa = 3.53EE292 pKa = 5.6LIRR295 pKa = 11.84EE296 pKa = 4.34FTYY299 pKa = 10.76EE300 pKa = 3.46EE301 pKa = 3.88WKK303 pKa = 10.8DD304 pKa = 3.05IEE306 pKa = 4.48RR307 pKa = 11.84YY308 pKa = 9.71HH309 pKa = 7.19IARR312 pKa = 11.84EE313 pKa = 4.02HH314 pKa = 6.45LGEE317 pKa = 5.16DD318 pKa = 3.8DD319 pKa = 3.72WDD321 pKa = 3.94DD322 pKa = 5.03FYY324 pKa = 11.83DD325 pKa = 3.9EE326 pKa = 4.64QLGGDD331 pKa = 4.08DD332 pKa = 4.69PYY334 pKa = 12.06ANDD337 pKa = 3.17FDD339 pKa = 4.28NRR341 pKa = 11.84EE342 pKa = 3.97EE343 pKa = 4.03VADD346 pKa = 4.18YY347 pKa = 11.54YY348 pKa = 11.56NALDD352 pKa = 3.77DD353 pKa = 4.94RR354 pKa = 11.84YY355 pKa = 11.05NEE357 pKa = 4.45AILRR361 pKa = 11.84FFGDD365 pKa = 3.05RR366 pKa = 11.84FVADD370 pKa = 4.39RR371 pKa = 11.84SGEE374 pKa = 4.01MTGRR378 pKa = 11.84EE379 pKa = 4.34SLNPAQYY386 pKa = 10.56AASVLANRR394 pKa = 11.84ARR396 pKa = 11.84HH397 pKa = 5.46VYY399 pKa = 10.5GFDD402 pKa = 3.25EE403 pKa = 6.64DD404 pKa = 4.37EE405 pKa = 4.61FEE407 pKa = 5.04SNGRR411 pKa = 11.84TIIVHH416 pKa = 6.41DD417 pKa = 4.38SKK419 pKa = 11.24ISGDD423 pKa = 3.26RR424 pKa = 11.84ALRR427 pKa = 11.84RR428 pKa = 11.84QEE430 pKa = 3.98NEE432 pKa = 3.38RR433 pKa = 11.84MADD436 pKa = 2.81IAAQRR441 pKa = 11.84KK442 pKa = 8.6ALLRR446 pKa = 11.84TRR448 pKa = 11.84DD449 pKa = 3.42QIASEE454 pKa = 3.92RR455 pKa = 11.84RR456 pKa = 11.84EE457 pKa = 4.15LEE459 pKa = 3.81RR460 pKa = 11.84QTRR463 pKa = 11.84SYY465 pKa = 11.26RR466 pKa = 11.84EE467 pKa = 3.35NLQRR471 pKa = 11.84KK472 pKa = 8.94RR473 pKa = 11.84EE474 pKa = 4.1ALKK477 pKa = 10.07EE478 pKa = 4.01AQIEE482 pKa = 4.06AQRR485 pKa = 11.84RR486 pKa = 11.84LKK488 pKa = 9.98EE489 pKa = 3.95LKK491 pKa = 9.17EE492 pKa = 4.05AKK494 pKa = 9.13TAFLKK499 pKa = 10.29EE500 pKa = 4.19HH501 pKa = 5.73EE502 pKa = 4.57AKK504 pKa = 10.59AAEE507 pKa = 4.42LDD509 pKa = 3.64RR510 pKa = 11.84AINAMSSSLPSEE522 pKa = 4.78SKK524 pKa = 10.99DD525 pKa = 3.15HH526 pKa = 4.82THH528 pKa = 6.09EE529 pKa = 4.17DD530 pKa = 3.3FRR532 pKa = 11.84RR533 pKa = 11.84RR534 pKa = 11.84LSPGQPEE541 pKa = 4.18PEE543 pKa = 4.18SPVKK547 pKa = 10.33GKK549 pKa = 10.52KK550 pKa = 7.43VTFQSQGQHH559 pKa = 4.99DD560 pKa = 4.51TKK562 pKa = 10.82NSKK565 pKa = 10.25PIEE568 pKa = 4.23TTQEE572 pKa = 4.07STGSKK577 pKa = 10.17APPSSSEE584 pKa = 3.95PKK586 pKa = 10.08RR587 pKa = 11.84PPLKK591 pKa = 9.81HH592 pKa = 6.46HH593 pKa = 6.69SPGSEE598 pKa = 3.68TGKK601 pKa = 10.15LPRR604 pKa = 11.84RR605 pKa = 11.84RR606 pKa = 11.84KK607 pKa = 9.46RR608 pKa = 11.84RR609 pKa = 11.84RR610 pKa = 11.84AASNRR615 pKa = 11.84KK616 pKa = 8.45KK617 pKa = 10.4QQ618 pKa = 3.25

Molecular weight: 69.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1496

432

618

498.7

55.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.024 ± 0.699

1.537 ± 0.386

5.882 ± 0.641

7.286 ± 0.567

3.275 ± 0.307

6.885 ± 0.314

2.607 ± 0.493

3.743 ± 0.281

5.214 ± 0.626

8.556 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.201

3.275 ± 0.216

5.414 ± 0.367

4.011 ± 0.277

8.021 ± 0.747

7.888 ± 0.148

4.479 ± 0.234

6.417 ± 0.842

1.404 ± 0.418

2.741 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski