Tortoise microvirus 68

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FW03|A0A4V1FW03_9VIRU Internal scaffolding protein OS=Tortoise microvirus 68 OX=2583174 PE=4 SV=1
MM1 pKa = 7.31EE2 pKa = 6.24AISFEE7 pKa = 4.15TQYY10 pKa = 11.1RR11 pKa = 11.84KK12 pKa = 9.72PKK14 pKa = 10.48RR15 pKa = 11.84FFTSEE20 pKa = 3.79GSPIKK25 pKa = 9.45ITYY28 pKa = 9.58GAVFDD33 pKa = 4.1NKK35 pKa = 10.37GRR37 pKa = 11.84LQLEE41 pKa = 4.51EE42 pKa = 4.56KK43 pKa = 10.67GSEE46 pKa = 3.91NLYY49 pKa = 10.93DD50 pKa = 4.07YY51 pKa = 10.21IQSFAEE57 pKa = 4.03SVDD60 pKa = 2.76IHH62 pKa = 7.1VILKK66 pKa = 10.26KK67 pKa = 10.22FANGDD72 pKa = 3.62STVLSRR78 pKa = 11.84AQGFYY83 pKa = 11.22GDD85 pKa = 3.69VTEE88 pKa = 6.12FPTTYY93 pKa = 11.01ADD95 pKa = 3.23VLNRR99 pKa = 11.84VIEE102 pKa = 4.26GEE104 pKa = 4.11NFFNTLPVEE113 pKa = 4.31TRR115 pKa = 11.84SKK117 pKa = 10.64FNHH120 pKa = 6.44SYY122 pKa = 11.14SEE124 pKa = 4.65FIASIGSQQFFDD136 pKa = 3.97AFGMSKK142 pKa = 10.47PSDD145 pKa = 3.68QNVVDD150 pKa = 5.06PVDD153 pKa = 4.09LPDD156 pKa = 3.36VVKK159 pKa = 10.39EE160 pKa = 4.26VKK162 pKa = 10.44SDD164 pKa = 3.43VV165 pKa = 3.41

Molecular weight: 18.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W7D7|A0A4P8W7D7_9VIRU Replication initiation protein OS=Tortoise microvirus 68 OX=2583174 PE=4 SV=1
MM1 pKa = 7.29SVSNGISTYY10 pKa = 10.3TPAPANQNMIQAVNSAANNIGMMQGIAQANSAASARR46 pKa = 11.84EE47 pKa = 3.67ARR49 pKa = 11.84IQRR52 pKa = 11.84EE53 pKa = 3.7WQEE56 pKa = 4.09RR57 pKa = 11.84QNARR61 pKa = 11.84AMAFNSLEE69 pKa = 4.0ASKK72 pKa = 11.11SRR74 pKa = 11.84DD75 pKa = 3.12WQQMMSNTAHH85 pKa = 5.68QRR87 pKa = 11.84EE88 pKa = 4.5IQDD91 pKa = 3.91LMAAGLNPVLSAMGGNGAAVTSGATASGVTSAGAKK126 pKa = 10.13GDD128 pKa = 3.31VDD130 pKa = 3.97TSANAAITNLLGSLLSAEE148 pKa = 4.46TSIKK152 pKa = 10.04AANINAQTQSAVADD166 pKa = 4.24KK167 pKa = 8.93YY168 pKa = 9.39TAMSQLVAEE177 pKa = 4.94LNAAASMYY185 pKa = 10.71SADD188 pKa = 3.44SHH190 pKa = 7.29AGASRR195 pKa = 11.84YY196 pKa = 9.68AADD199 pKa = 3.86RR200 pKa = 11.84SAAASMFGSSLMSAASKK217 pKa = 10.26HH218 pKa = 6.09AADD221 pKa = 3.73QSYY224 pKa = 9.42EE225 pKa = 3.73ASRR228 pKa = 11.84YY229 pKa = 7.65RR230 pKa = 11.84ANMDD234 pKa = 2.96WSITNEE240 pKa = 4.03KK241 pKa = 9.63NIHH244 pKa = 5.69DD245 pKa = 5.54AIVKK249 pKa = 9.95DD250 pKa = 3.96LYY252 pKa = 11.6GNNQYY257 pKa = 9.95TGIFGWILSKK267 pKa = 10.58WPIFDD272 pKa = 3.9YY273 pKa = 10.74SSQRR277 pKa = 11.84RR278 pKa = 11.84MFSKK282 pKa = 10.7RR283 pKa = 3.14

Molecular weight: 30.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1446

83

568

289.2

32.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.437 ± 2.204

0.899 ± 0.559

6.432 ± 0.543

4.91 ± 1.039

4.703 ± 0.833

6.155 ± 0.176

1.936 ± 0.263

5.533 ± 0.46

4.495 ± 1.003

6.777 ± 0.656

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.702

4.979 ± 0.607

4.011 ± 0.769

4.219 ± 0.733

5.394 ± 0.542

9.959 ± 0.979

5.878 ± 0.912

6.501 ± 1.465

1.383 ± 0.399

4.979 ± 0.591
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski