Ketogulonicigenium vulgare (strain WSH-001)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ketogulonicigenium; Ketogulonicigenium vulgare

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9Y6Q7|F9Y6Q7_KETVW Isochorismatase hydrolase OS=Ketogulonicigenium vulgare (strain WSH-001) OX=759362 GN=pncA PE=4 SV=1
MM1 pKa = 7.48LGKK4 pKa = 10.19LAFSGMQHH12 pKa = 6.41EE13 pKa = 4.73GHH15 pKa = 5.65FMKK18 pKa = 10.89RR19 pKa = 11.84ILLASAALTAFAGAAAAEE37 pKa = 4.21ISFNGDD43 pKa = 2.29ARR45 pKa = 11.84LGYY48 pKa = 10.58NDD50 pKa = 3.84TAIEE54 pKa = 3.83NDD56 pKa = 3.08GFYY59 pKa = 10.34WRR61 pKa = 11.84LRR63 pKa = 11.84MNIAASQEE71 pKa = 4.0LDD73 pKa = 3.06NGLTFGGSVRR83 pKa = 11.84FDD85 pKa = 4.97LIDD88 pKa = 3.44NGYY91 pKa = 10.09ISDD94 pKa = 4.48PALDD98 pKa = 3.89TNNLKK103 pKa = 10.77LFLEE107 pKa = 4.5AGEE110 pKa = 4.42LGGLYY115 pKa = 10.5FGDD118 pKa = 4.17YY119 pKa = 10.32EE120 pKa = 4.69FSAVDD125 pKa = 3.08AWKK128 pKa = 10.22AAGPMFADD136 pKa = 5.09GFSEE140 pKa = 4.19QDD142 pKa = 3.09GEE144 pKa = 4.24KK145 pKa = 9.81TLRR148 pKa = 11.84GDD150 pKa = 3.16INYY153 pKa = 10.01GNVAVSVSTPISSTTGNDD171 pKa = 3.21NASEE175 pKa = 4.21SQLGQWNFAASTTFGAFDD193 pKa = 3.8VVFAYY198 pKa = 10.23QGEE201 pKa = 4.23GDD203 pKa = 3.88DD204 pKa = 6.02DD205 pKa = 4.58YY206 pKa = 11.55FAEE209 pKa = 4.76AALGDD214 pKa = 4.74DD215 pKa = 4.06INPNEE220 pKa = 4.49IIGLSVGTTFAGASVRR236 pKa = 11.84LAYY239 pKa = 8.67ATTDD243 pKa = 3.39YY244 pKa = 10.93KK245 pKa = 11.38VAGTTQSSLGLTATYY260 pKa = 10.34PLGPVALTGWVIFEE274 pKa = 5.4DD275 pKa = 4.64GDD277 pKa = 3.49VSDD280 pKa = 6.17AMDD283 pKa = 3.97EE284 pKa = 4.09DD285 pKa = 4.47LFYY288 pKa = 11.01GARR291 pKa = 11.84VAYY294 pKa = 9.31AANGFSISADD304 pKa = 3.4YY305 pKa = 11.29GEE307 pKa = 4.93RR308 pKa = 11.84LANNLSIGGRR318 pKa = 11.84DD319 pKa = 3.13ARR321 pKa = 11.84LAVEE325 pKa = 4.69GGYY328 pKa = 10.83DD329 pKa = 3.62LGNGINLKK337 pKa = 10.4AGYY340 pKa = 9.73LSRR343 pKa = 11.84DD344 pKa = 2.94SWSIGSGEE352 pKa = 4.06AYY354 pKa = 10.44YY355 pKa = 11.12VGGTYY360 pKa = 10.78DD361 pKa = 3.98LGAGATLLVSYY372 pKa = 10.99ADD374 pKa = 4.59ADD376 pKa = 4.01DD377 pKa = 4.0TSAGYY382 pKa = 10.78LSDD385 pKa = 5.18DD386 pKa = 3.8EE387 pKa = 4.93VGAPAYY393 pKa = 10.45QNGATIEE400 pKa = 4.12VSFKK404 pKa = 10.8FF405 pKa = 3.84

Molecular weight: 42.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9Y5F4|F9Y5F4_KETVW Periplasmic protein thiol:disulfide oxidoreductase DsbE subfamily protein OS=Ketogulonicigenium vulgare (strain WSH-001) OX=759362 GN=cycY PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3049

0

3049

950172

23

2789

311.6

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.121 ± 0.074

0.71 ± 0.014

5.962 ± 0.036

4.955 ± 0.038

3.682 ± 0.027

8.591 ± 0.04

2.065 ± 0.022

5.554 ± 0.029

2.514 ± 0.035

10.347 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.022

2.659 ± 0.029

5.231 ± 0.031

3.615 ± 0.022

6.798 ± 0.042

5.066 ± 0.033

5.553 ± 0.031

7.183 ± 0.034

1.354 ± 0.019

2.242 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski