Pontibacter sp. 2b14

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter; unclassified Pontibacter

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A364RED8|A0A364RED8_9BACT TPR_REGION domain-containing protein OS=Pontibacter sp. 2b14 OX=2080288 GN=DP923_09705 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.52KK3 pKa = 10.27NLFFAAAFMLFAGAAVAQGNTNTVNQSGTNHH34 pKa = 6.04NAEE37 pKa = 4.3VAQTGARR44 pKa = 11.84NYY46 pKa = 10.83SAVDD50 pKa = 3.27QTNDD54 pKa = 3.1NNNAIVDD61 pKa = 3.83QLGTDD66 pKa = 3.34NKK68 pKa = 10.95SEE70 pKa = 3.88IVQIQGAYY78 pKa = 9.78NNEE81 pKa = 3.77ATVDD85 pKa = 3.53QEE87 pKa = 4.37GASNIAYY94 pKa = 8.24QQQGGNATSNVADD107 pKa = 3.74VMQVGDD113 pKa = 4.5NNNALQSQVINSGNNEE129 pKa = 3.78AFIGQFGDD137 pKa = 3.32NNRR140 pKa = 11.84ASQYY144 pKa = 10.73QEE146 pKa = 3.93ASSDD150 pKa = 3.7YY151 pKa = 10.04NLAEE155 pKa = 3.97IVQDD159 pKa = 3.59GTRR162 pKa = 11.84NLANQDD168 pKa = 3.26QSDD171 pKa = 4.03VSGSNQAYY179 pKa = 9.59IDD181 pKa = 3.62QTGDD185 pKa = 3.26RR186 pKa = 11.84NDD188 pKa = 3.6AAQEE192 pKa = 3.9QTNVSLGNMATVTQEE207 pKa = 4.63GYY209 pKa = 9.33WNYY212 pKa = 9.91GFQYY216 pKa = 10.47QRR218 pKa = 11.84GISALNEE225 pKa = 3.67ATITQTGDD233 pKa = 2.77INDD236 pKa = 3.93AYY238 pKa = 10.58QYY240 pKa = 11.15QEE242 pKa = 5.11LISLANVATITQTGDD257 pKa = 2.93YY258 pKa = 10.16NYY260 pKa = 10.82ARR262 pKa = 11.84QEE264 pKa = 3.9QSSLSGLFNEE274 pKa = 4.83ATITQTGDD282 pKa = 3.0SNRR285 pKa = 11.84GFQTQRR291 pKa = 11.84NGGDD295 pKa = 3.16LTATLTQDD303 pKa = 3.21GNSNLSYY310 pKa = 9.39QTQTGTSHH318 pKa = 6.51VSTVTQTGSFNTSTVTQGNN337 pKa = 3.59

Molecular weight: 36.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A364RCD9|A0A364RCD9_9BACT FRG domain-containing protein OS=Pontibacter sp. 2b14 OX=2080288 GN=DP923_14775 PE=4 SV=1
MM1 pKa = 7.69LNRR4 pKa = 11.84KK5 pKa = 9.37RR6 pKa = 11.84PLQVTNGLEE15 pKa = 4.26FVVTAVPQQTYY26 pKa = 11.13ANAGLYY32 pKa = 10.21LRR34 pKa = 11.84IVSIGYY40 pKa = 8.47FEE42 pKa = 5.7KK43 pKa = 10.69KK44 pKa = 10.16DD45 pKa = 3.64LPLPCNSAICATILTKK61 pKa = 9.42HH62 pKa = 4.63TRR64 pKa = 11.84FRR66 pKa = 11.84SLHH69 pKa = 5.29FPFRR73 pKa = 11.84VCSSLAPRR81 pKa = 11.84LSQQ84 pKa = 3.95

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3170

0

3170

1063188

25

2614

335.4

37.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.231 ± 0.044

0.711 ± 0.012

5.059 ± 0.03

6.382 ± 0.041

4.586 ± 0.036

6.77 ± 0.043

1.906 ± 0.021

6.527 ± 0.043

6.375 ± 0.037

9.999 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.018

5.028 ± 0.039

3.974 ± 0.027

4.248 ± 0.036

4.293 ± 0.031

5.963 ± 0.035

6.033 ± 0.049

6.625 ± 0.029

1.022 ± 0.015

3.925 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski