Hubei macula-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK41|A0A1L3KK41_9VIRU Tymo_coat domain-containing protein OS=Hubei macula-like virus 1 OX=1922922 PE=4 SV=1
MM1 pKa = 8.01DD2 pKa = 3.43AHH4 pKa = 6.01EE5 pKa = 4.38TLFVSSKK12 pKa = 9.22TKK14 pKa = 10.07SSSRR18 pKa = 11.84GLIAHH23 pKa = 6.86NFQSINHH30 pKa = 5.78NPRR33 pKa = 11.84LIMDD37 pKa = 3.72QLILLLKK44 pKa = 10.19PLLDD48 pKa = 3.8KK49 pKa = 11.2LLLPSEE55 pKa = 4.45EE56 pKa = 4.08AATPEE61 pKa = 4.25VSPPPPSIAPPKK73 pKa = 10.69APALSNNPVLPALPVPRR90 pKa = 11.84FSGHH94 pKa = 7.12LPQTAGNQGVLIPFQVSVFHH114 pKa = 6.98INAASDD120 pKa = 3.57SASSYY125 pKa = 9.5TIRR128 pKa = 11.84EE129 pKa = 3.98LSAVQTLLPYY139 pKa = 10.5FRR141 pKa = 11.84DD142 pKa = 3.82VVVKK146 pKa = 10.35QADD149 pKa = 3.74VVVFPTVASKK159 pKa = 8.7TVPASIDD166 pKa = 3.76LCWSPSYY173 pKa = 10.47KK174 pKa = 10.05ILGSEE179 pKa = 4.33VLSTPSSTRR188 pKa = 11.84FNIGLDD194 pKa = 3.38PALISSSLPCDD205 pKa = 3.95FGHH208 pKa = 7.01INPIIKK214 pKa = 10.41SPIPYY219 pKa = 9.38DD220 pKa = 3.22DD221 pKa = 4.37HH222 pKa = 6.79PRR224 pKa = 11.84LNIKK228 pKa = 9.87IYY230 pKa = 9.7QSSGASSSVPLGEE243 pKa = 4.45LIIRR247 pKa = 11.84GILQCSNPLPNN258 pKa = 4.16

Molecular weight: 27.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK41|A0A1L3KK41_9VIRU Tymo_coat domain-containing protein OS=Hubei macula-like virus 1 OX=1922922 PE=4 SV=1
MM1 pKa = 7.57APSTMQRR8 pKa = 11.84YY9 pKa = 8.38LQILATDD16 pKa = 3.77AMAFSSQRR24 pKa = 11.84KK25 pKa = 6.52VVVYY29 pKa = 8.4VWVYY33 pKa = 8.16PSQRR37 pKa = 11.84KK38 pKa = 7.98FFPSRR43 pKa = 11.84HH44 pKa = 5.92PIIDD48 pKa = 3.59RR49 pKa = 11.84GRR51 pKa = 11.84VIYY54 pKa = 10.64SDD56 pKa = 3.53AVRR59 pKa = 11.84ALLLDD64 pKa = 3.69NVFQTLLSPQSTTSDD79 pKa = 2.81IATLATSLGASLDD92 pKa = 3.61DD93 pKa = 3.6VGIFFSFSCSFSPVPRR109 pKa = 11.84KK110 pKa = 9.82SS111 pKa = 2.95

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1947

111

1578

649.0

73.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.653 ± 0.194

1.387 ± 0.147

5.085 ± 0.238

3.955 ± 1.031

5.085 ± 0.452

3.595 ± 0.135

3.903 ± 0.853

5.65 ± 0.821

4.109 ± 0.312

11.402 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.489 ± 0.217

4.366 ± 0.59

7.756 ± 1.312

4.109 ± 0.21

5.29 ± 0.614

9.656 ± 1.967

5.085 ± 0.337

5.65 ± 0.953

1.079 ± 0.255

3.698 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski