Sphingomonas gilva

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396RSC3|A0A396RSC3_9SPHN Arylsulfatase OS=Sphingomonas gilva OX=2305907 GN=D1610_04180 PE=3 SV=1
MM1 pKa = 7.61AATPALAQDD10 pKa = 3.81TQDD13 pKa = 3.44PSVAPEE19 pKa = 3.89TQTAEE24 pKa = 4.41DD25 pKa = 4.59ADD27 pKa = 3.88TGEE30 pKa = 4.76NIVVTGSILRR40 pKa = 11.84RR41 pKa = 11.84STADD45 pKa = 2.98TVSPITVVTADD56 pKa = 4.14DD57 pKa = 3.88LDD59 pKa = 3.96ARR61 pKa = 11.84GINTTQEE68 pKa = 4.19AIQRR72 pKa = 11.84LSSNNGPALTNSFSANGAFAAGASAVSLRR101 pKa = 11.84GLSTSSTLVLFDD113 pKa = 3.59GMRR116 pKa = 11.84ASYY119 pKa = 10.86YY120 pKa = 10.12PLADD124 pKa = 3.79DD125 pKa = 3.57ATRR128 pKa = 11.84NFVDD132 pKa = 5.16LNTIPDD138 pKa = 4.74DD139 pKa = 3.19IVEE142 pKa = 4.21RR143 pKa = 11.84VEE145 pKa = 3.88VLRR148 pKa = 11.84DD149 pKa = 3.72GASSTYY155 pKa = 10.5GADD158 pKa = 3.69AIAGVVNVITKK169 pKa = 10.36RR170 pKa = 11.84EE171 pKa = 3.87ITGISARR178 pKa = 11.84AEE180 pKa = 3.78AGIDD184 pKa = 3.45EE185 pKa = 5.23RR186 pKa = 11.84GNSPNYY192 pKa = 9.84RR193 pKa = 11.84LSLTGGFGDD202 pKa = 4.93VEE204 pKa = 4.25GDD206 pKa = 3.64GYY208 pKa = 11.62NVYY211 pKa = 11.02ASGFYY216 pKa = 8.61YY217 pKa = 9.99TQDD220 pKa = 3.05ALFNGDD226 pKa = 4.51LPYY229 pKa = 10.48PYY231 pKa = 10.4NSQNQSGICLDD242 pKa = 4.44GVCGPQVGPNSGSINPSSQGFEE264 pKa = 4.23GFSLTSDD271 pKa = 2.82RR272 pKa = 11.84RR273 pKa = 11.84LFATTFFVAPYY284 pKa = 9.29DD285 pKa = 3.86ASNTTSQGRR294 pKa = 11.84YY295 pKa = 7.24QFLNPAAGCITGEE308 pKa = 4.12TPYY311 pKa = 10.04TLSAAEE317 pKa = 4.89FADD320 pKa = 4.07PDD322 pKa = 3.74NALSPRR328 pKa = 11.84TVCQGDD334 pKa = 4.2LVTQYY339 pKa = 11.44GVVSPEE345 pKa = 3.77LEE347 pKa = 4.07RR348 pKa = 11.84FGGAIKK354 pKa = 9.39GTIALGGGIEE364 pKa = 4.05ASLVGNYY371 pKa = 8.68LQSKK375 pKa = 9.09VSYY378 pKa = 8.8TGEE381 pKa = 3.98PATIRR386 pKa = 11.84ANANTGIMFPRR397 pKa = 11.84FSTFTGGPPNAPGSFALALPVYY419 pKa = 9.76VCPLVNGMPQATCDD433 pKa = 3.61ATNGTLNPNNPFAAQGQVARR453 pKa = 11.84IIGRR457 pKa = 11.84IPNLIEE463 pKa = 3.92YY464 pKa = 10.45NEE466 pKa = 4.36TLSRR470 pKa = 11.84TYY472 pKa = 10.53RR473 pKa = 11.84GALTISGPISDD484 pKa = 3.96NWNFAVDD491 pKa = 3.9AVGMRR496 pKa = 11.84TDD498 pKa = 3.89LRR500 pKa = 11.84RR501 pKa = 11.84TQEE504 pKa = 4.07GYY506 pKa = 11.26VYY508 pKa = 10.29IQRR511 pKa = 11.84LLNVVADD518 pKa = 4.26GSYY521 pKa = 11.19NFVDD525 pKa = 4.43PFANSQATLDD535 pKa = 3.91YY536 pKa = 10.83LSPDD540 pKa = 3.3NVTDD544 pKa = 3.58SHH546 pKa = 8.15SDD548 pKa = 2.89IYY550 pKa = 11.1AASANISGSLFEE562 pKa = 5.33LPGGPLQLGVGAAIRR577 pKa = 11.84NEE579 pKa = 4.44SIDD582 pKa = 3.8APSANDD588 pKa = 3.84DD589 pKa = 3.65FAGPTEE595 pKa = 4.42RR596 pKa = 11.84YY597 pKa = 8.86FVLNAFGTSGEE608 pKa = 4.17RR609 pKa = 11.84TVYY612 pKa = 10.63SAFAEE617 pKa = 3.91LDD619 pKa = 3.54APILEE624 pKa = 4.39WVDD627 pKa = 3.44VNLSGRR633 pKa = 11.84YY634 pKa = 9.37DD635 pKa = 3.64NYY637 pKa = 11.33SSGQDD642 pKa = 2.98AFSPKK647 pKa = 9.44VGVRR651 pKa = 11.84FRR653 pKa = 11.84PFDD656 pKa = 3.6GLTVRR661 pKa = 11.84GTWSRR666 pKa = 11.84GFRR669 pKa = 11.84IPSFAEE675 pKa = 3.8ANALPTTGFVTNTAALFNDD694 pKa = 4.64TYY696 pKa = 10.83LAQYY700 pKa = 9.2GCTVATFNACPTYY713 pKa = 10.46IRR715 pKa = 11.84AGAYY719 pKa = 8.88GQTTLASPDD728 pKa = 3.86LDD730 pKa = 3.95PEE732 pKa = 4.69RR733 pKa = 11.84STSWTAGIVFEE744 pKa = 4.36PLRR747 pKa = 11.84NVTLAVDD754 pKa = 3.71YY755 pKa = 11.44YY756 pKa = 10.99NIKK759 pKa = 8.7KK760 pKa = 8.47TGAITTPSNSPALIAYY776 pKa = 8.89YY777 pKa = 10.06SGQAIPEE784 pKa = 4.62GYY786 pKa = 10.27NVIADD791 pKa = 3.85APDD794 pKa = 3.39AQFPNATPRR803 pKa = 11.84VAFVQSSLINANTIRR818 pKa = 11.84SEE820 pKa = 4.13GLDD823 pKa = 3.5FAARR827 pKa = 11.84ASFDD831 pKa = 3.8LGPDD835 pKa = 2.89IRR837 pKa = 11.84FTSSAEE843 pKa = 3.31ASYY846 pKa = 10.45IINLSTEE853 pKa = 4.1FPDD856 pKa = 4.77GSVEE860 pKa = 4.32SYY862 pKa = 10.88EE863 pKa = 4.13GTLGNFNLTAGSGTPEE879 pKa = 3.73WHH881 pKa = 7.07GSWQNTLEE889 pKa = 4.02MGAWTLTATAEE900 pKa = 4.17YY901 pKa = 10.25FDD903 pKa = 4.97GYY905 pKa = 11.0NLSAEE910 pKa = 4.36DD911 pKa = 3.48QNGAGTSGDD920 pKa = 3.73CGLLSEE926 pKa = 5.46AFVEE930 pKa = 4.5CDD932 pKa = 3.1VPSYY936 pKa = 10.2VTVDD940 pKa = 3.69LSTSFDD946 pKa = 3.62VNDD949 pKa = 3.38NFTFYY954 pKa = 11.72VNVLNVFDD962 pKa = 5.03NLPPIDD968 pKa = 5.35PITYY972 pKa = 9.46GANNYY977 pKa = 10.07NPVQGGTGIFGRR989 pKa = 11.84SFRR992 pKa = 11.84AGAKK996 pKa = 10.22VNFF999 pKa = 4.21

Molecular weight: 105.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396RRU7|A0A396RRU7_9SPHN p-hydroxycinnamoyl CoA hydratase/lyase OS=Sphingomonas gilva OX=2305907 GN=D1610_03945 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3257

0

3257

1067601

30

3520

327.8

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.045 ± 0.073

0.695 ± 0.012

6.145 ± 0.035

5.574 ± 0.042

3.498 ± 0.029

9.225 ± 0.052

1.896 ± 0.025

4.943 ± 0.029

2.666 ± 0.038

9.595 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.025

2.422 ± 0.032

5.463 ± 0.034

2.879 ± 0.024

7.755 ± 0.052

4.85 ± 0.038

5.218 ± 0.047

7.12 ± 0.036

1.469 ± 0.019

2.211 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski