Gordonia terrae phage McKinley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EL95|A0A4Y6EL95_9CAUD Uncharacterized protein OS=Gordonia terrae phage McKinley OX=2588507 GN=71 PE=4 SV=1
MM1 pKa = 7.69PSDD4 pKa = 5.56DD5 pKa = 4.07IRR7 pKa = 11.84NYY9 pKa = 11.28DD10 pKa = 3.81PDD12 pKa = 3.28TGMLYY17 pKa = 9.88RR18 pKa = 11.84WEE20 pKa = 4.57MPDD23 pKa = 3.11SPRR26 pKa = 11.84GFVIEE31 pKa = 5.19IEE33 pKa = 4.23NDD35 pKa = 3.19DD36 pKa = 5.32DD37 pKa = 5.78GPILCGCVTGPGFTKK52 pKa = 10.43PAGFLYY58 pKa = 9.89PAEE61 pKa = 4.29ARR63 pKa = 11.84WLAEE67 pKa = 3.93NMPSMLASIEE77 pKa = 4.22AYY79 pKa = 9.66EE80 pKa = 4.16LQQGATSS87 pKa = 3.39

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EPJ3|A0A4Y6EPJ3_9CAUD Head-to-tail adaptor OS=Gordonia terrae phage McKinley OX=2588507 GN=29 PE=4 SV=1
MM1 pKa = 7.26GWLLFGAVFIPVRR14 pKa = 11.84IAKK17 pKa = 9.9IIEE20 pKa = 4.48DD21 pKa = 3.61ALSPRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.9LIQQQTRR37 pKa = 11.84DD38 pKa = 3.68EE39 pKa = 4.24VAAAAEE45 pKa = 3.96RR46 pKa = 11.84SEE48 pKa = 4.23EE49 pKa = 3.96YY50 pKa = 10.34RR51 pKa = 11.84RR52 pKa = 11.84TRR54 pKa = 11.84YY55 pKa = 10.12GLL57 pKa = 3.49

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

18753

36

1607

213.1

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.211 ± 0.395

0.789 ± 0.119

7.476 ± 0.482

5.546 ± 0.36

2.517 ± 0.159

8.596 ± 0.521

2.133 ± 0.182

4.501 ± 0.262

2.581 ± 0.208

7.609 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.113

2.688 ± 0.168

6.378 ± 0.233

3.717 ± 0.183

7.343 ± 0.413

4.922 ± 0.215

6.644 ± 0.25

7.796 ± 0.25

2.181 ± 0.137

2.208 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski