Methylacidimicrobium cyclopophantes

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobia incertae sedis; Methylacidimicrobium

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E6MGJ9|A0A5E6MGJ9_9BACT Glycolate oxidase iron-sulfur subunit OS=Methylacidimicrobium cyclopophantes OX=1041766 GN=glcF PE=4 SV=1
MM1 pKa = 7.82ADD3 pKa = 3.29LTNRR7 pKa = 11.84YY8 pKa = 8.09PDD10 pKa = 3.53NVAGKK15 pKa = 10.13YY16 pKa = 9.6YY17 pKa = 10.81VDD19 pKa = 4.24SQCIDD24 pKa = 3.56CDD26 pKa = 3.82LCRR29 pKa = 11.84EE30 pKa = 4.16TAPSNFKK37 pKa = 10.94RR38 pKa = 11.84NDD40 pKa = 3.85DD41 pKa = 4.0GGHH44 pKa = 5.6SYY46 pKa = 10.95VYY48 pKa = 9.84KK49 pKa = 10.56QPEE52 pKa = 4.14TPEE55 pKa = 4.34EE56 pKa = 4.02EE57 pKa = 4.43EE58 pKa = 3.84QCRR61 pKa = 11.84EE62 pKa = 4.08AKK64 pKa = 10.05EE65 pKa = 4.2GCPVEE70 pKa = 5.57AIGDD74 pKa = 3.94DD75 pKa = 4.12GEE77 pKa = 4.24LAEE80 pKa = 5.52AATQTTEE87 pKa = 3.65ARR89 pKa = 11.84EE90 pKa = 4.2GG91 pKa = 3.44

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E6MJ48|A0A5E6MJ48_9BACT Uncharacterized protein OS=Methylacidimicrobium cyclopophantes OX=1041766 GN=MAMC_00939 PE=4 SV=1
MM1 pKa = 7.35PVARR5 pKa = 11.84EE6 pKa = 4.1VSGSPPPLSGTNPSSVLTRR25 pKa = 11.84SEE27 pKa = 3.67QRR29 pKa = 11.84RR30 pKa = 11.84IAAICRR36 pKa = 11.84RR37 pKa = 11.84LAEE40 pKa = 4.97AYY42 pKa = 9.55PEE44 pKa = 4.1TRR46 pKa = 11.84PALHH50 pKa = 6.07YY51 pKa = 10.35RR52 pKa = 11.84NPLEE56 pKa = 4.65LLIATILSARR66 pKa = 11.84CTDD69 pKa = 3.42RR70 pKa = 11.84QVNRR74 pKa = 11.84VTADD78 pKa = 2.75IFSRR82 pKa = 11.84FPDD85 pKa = 3.62AASYY89 pKa = 10.31AAASQEE95 pKa = 4.01EE96 pKa = 4.53MEE98 pKa = 4.49EE99 pKa = 3.88ALKK102 pKa = 10.85RR103 pKa = 11.84LGFFRR108 pKa = 11.84SKK110 pKa = 10.45ARR112 pKa = 11.84HH113 pKa = 4.9IRR115 pKa = 11.84RR116 pKa = 11.84TAAILTEE123 pKa = 4.1RR124 pKa = 11.84WGGRR128 pKa = 11.84VPKK131 pKa = 10.26TMEE134 pKa = 4.49EE135 pKa = 4.16LTEE138 pKa = 4.08LPGVGRR144 pKa = 11.84KK145 pKa = 6.7TANVVLGNGFGLPLGIVVDD164 pKa = 3.57THH166 pKa = 5.51VARR169 pKa = 11.84VSYY172 pKa = 10.63RR173 pKa = 11.84LGLTDD178 pKa = 3.24QKK180 pKa = 10.85LPKK183 pKa = 9.99RR184 pKa = 11.84IEE186 pKa = 3.76IDD188 pKa = 3.04LMQRR192 pKa = 11.84IPRR195 pKa = 11.84RR196 pKa = 11.84EE197 pKa = 3.64WIAFSNRR204 pKa = 11.84LIFHH208 pKa = 5.56GRR210 pKa = 11.84KK211 pKa = 8.64RR212 pKa = 11.84CRR214 pKa = 11.84ARR216 pKa = 11.84KK217 pKa = 8.81PDD219 pKa = 3.93CLHH222 pKa = 6.97CEE224 pKa = 4.1LLSLCPRR231 pKa = 11.84QGLPPLSSSAGG242 pKa = 3.49

Molecular weight: 27.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2067

0

2067

655861

29

2475

317.3

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.429 ± 0.058

1.105 ± 0.018

4.212 ± 0.032

7.275 ± 0.065

4.168 ± 0.041

8.481 ± 0.053

1.956 ± 0.022

4.685 ± 0.037

3.321 ± 0.039

11.729 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.714 ± 0.019

2.2 ± 0.034

5.878 ± 0.04

3.073 ± 0.03

8.63 ± 0.061

6.217 ± 0.038

4.171 ± 0.033

6.796 ± 0.042

1.669 ± 0.026

2.291 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski