Proteome-pI 2.0 - Proteome Isoelectric Point Database

Spirochaeta sp. LUC14_002_19_P3

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Spirochaeta; unclassified Spirochaeta

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1W9N721|A0A1W9N721_9SPIO Nucleotidyltransferase OS=Spirochaeta sp. LUC14_002_19_P3 OX=1940821 GN=B0D92_07115 PE=4 SV=1

MM1 pKa = 7.94ASDD4 pKa = 4.05QDD6 pKa = 4.17VKK8 pKa = 11.29DD9 pKa = 3.68ALYY12 pKa = 10.91DD13 pKa = 3.91VIDD16 pKa = 4.69PEE18 pKa = 4.83LNMNIVDD25 pKa = 4.37LGLVYY30 pKa = 10.31RR31 pKa = 11.84AQVNEE36 pKa = 4.08DD37 pKa = 3.66NIAEE41 pKa = 4.08VDD43 pKa = 4.09FTLTYY48 pKa = 9.52PGCPMSDD55 pKa = 3.75MIASDD60 pKa = 3.7IEE62 pKa = 4.54HH63 pKa = 5.68QVKK66 pKa = 8.08TQTDD70 pKa = 3.81VEE72 pKa = 4.34AVRR75 pKa = 11.84VNLVWYY81 pKa = 7.47PPWSQDD87 pKa = 3.06MMSEE91 pKa = 4.06NARR94 pKa = 11.84LLMGYY99 pKa = 9.24PII101 pKa = 4.64

Molecular weight: 11.48 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1W9N771|A0A1W9N771_9SPIO Methyltransferase OS=Spirochaeta sp. LUC14_002_19_P3 OX=1940821 GN=B0D92_07080 PE=3 SV=1

MM1 pKa = 7.12EE2 pKa = 4.43TRR4 pKa = 11.84NVQTLCLSILTVLALGATLHH24 pKa = 6.28FLRR27 pKa = 11.84IVFLPLFIAGLLALMLTPLVQRR49 pKa = 11.84MEE51 pKa = 4.22KK52 pKa = 10.51LRR54 pKa = 11.84IPRR57 pKa = 11.84IFGIFLVMGALLILFFVVGRR77 pKa = 11.84MFYY80 pKa = 10.58TSLQTFTEE88 pKa = 4.29EE89 pKa = 3.8FGDD92 pKa = 3.81YY93 pKa = 9.98QKK95 pKa = 11.44RR96 pKa = 11.84LVSILNGLWEE106 pKa = 4.11QFNIPRR112 pKa = 11.84EE113 pKa = 4.08FFPKK117 pKa = 9.61FTWTRR122 pKa = 11.84DD123 pKa = 3.03IINRR127 pKa = 11.84IIQVTGSFVVYY138 pKa = 9.51WRR140 pKa = 11.84TDD142 pKa = 2.81AFSRR146 pKa = 11.84TGLRR150 pKa = 11.84LRR152 pKa = 11.84RR153 pKa = 11.84QAIWNGDD160 pKa = 3.47HH161 pKa = 6.54SKK163 pKa = 10.7GWAGSILSEE172 pKa = 4.01MFLFF176 pKa = 5.11

Molecular weight: 20.53 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1687	0	1687	565149	47	3249	335.0	37.16

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.123 ± 0.067	0.966 ± 0.021	5.153 ± 0.051	6.755 ± 0.055	4.183 ± 0.038	7.667 ± 0.057	1.869 ± 0.025	6.845 ± 0.059	5.248 ± 0.057	10.321 ± 0.067

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.44 ± 0.025	4.026 ± 0.043	4.453 ± 0.05	3.094 ± 0.028	5.855 ± 0.054	6.533 ± 0.04	5.039 ± 0.05	5.979 ± 0.047	1.287 ± 0.026	3.164 ± 0.037

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski